PALD encoding a lipid droplet‐associated protein is critical for the accumulation of lipid droplets and pollen longevity in Arabidopsis

Abstract

Pollen longevity is critical for plant pollination and hybrid seed production, but few studies have focused on pollen longevity.In this study, we identified an Arabidopsis thaliana gene, Protein associated with lipid droplets (PALD), which is strongly expressed in pollen and critical for the regulation of pollen longevity.PALD was expressed specifically in mature pollen grains and the pollen tube, and its expression was upregulated under dry conditions. PALD encoded a lipid droplet (LD)-associated protein and its N terminus was critical for the LD localization of PALD. The number of LDs and diameter were reduced in pollen grains of the loss-of-function PALD mutants. The viability and germination of the mature pollen grains of the pald mutants were comparable with those of the wild-type, but after the pollen grains were stored under dry conditions, pollen germination and male transmission of the mutant were compromised compared with those of the wild-type.Our study suggests that PALD was required for the maintenance of LD quality in mature pollen grains and regulation of pollen longevity.

Plasticity of the Arabidopsis leaf lipidome and proteome in response to pathogen infection and heat stress

“…We therefore obtained an enriched LD fraction from leaves of tgd1-1 sdp1-4 mutant Arabidopsis plants and investigated the proteome using quantitative label-free proteomics. First, to evaluate the success of LD enrichment, we combined the riBAQ values of all known detected LD proteins (Gidda et al, 2016; Brocard et al, 2017; Pyc et al, 2017; Kretzschmar et al, 2018; Fernández-Santos et al, 2020; Kretzschmar et al, 2020; Doner et al, 2021; Ge et al, 2022; Li et al, 2022) revealing a strong enrichment of this combined abundance by a factor of 65 to 775 in the LD fractions of the different treatment conditions (Suppl. Figure S10).…”

“…While the number of known LD-associated plant protein families has steadily increased in recent years (Fernández-Santos et al, 2020; Kretzschmar et al, 2020; Doner et al, 2021; Ge et al, 2022; Krawczyk et al, 2022b; Li et al, 2022), the number is still only roughly two dozen (Guzha et al, 2023). These studies were often followed up by the functional characterizations of these proteins, greatly increasing our understanding of LDs (Shimada et al, 2014a; Gidda et al, 2016; Deruyffelaere et al, 2018; Kretzschmar et al, 2018; Pyc et al, 2021; Krawczyk et al, 2022b).…”

“…Protein localization was annotated based on the Plant Proteome Database (Sun et al, 2009) as of 14 th June 2022. LD localization was assigned based on previous studies (Kretzschmar et al, 2018; Fernández-Santos et al, 2020; Kretzschmar et al, 2020; Doner et al, 2021; Ge et al, 2022; Li et al, 2022). Volcano plots were calculated in Perseus.…”

Genome-edited TaTFL1-5 mutation decreases tiller and spikelet numbers in common wheat

“…The primers TaTFL1 -5s-F, TaTFL1 -5s-F0, TaTFL1 -5s-R, and TaTFL1 -5s-R0 ( Table S1 ) was used for PCR amplification and pCBC-MT1T2 was used as the template. The PCR fragment was purified and inserted into expression vector pBUE411-Cas9, as described by Li et al. (2022) .…”

“…The primers TaTFL1-5s-F, TaTFL1-5s-F0, TaTFL1-5s-R, and TaTFL1-5s-R0 (Table S1) was used for PCR amplification and pCBC-MT1T2 was used as the template. The PCR fragment was purified and inserted into expression vector pBUE411-Cas9, as described by Li et al (2022). The callus cultured from immature embryo of Fielder was used for transformation by Agrobacterium tumefaciens-mediated method (PureWheat), as described by Medveckáand Harwood (2015).…”