<i>Pappenheim</i>-Färbung: Beschreibung einer hämatologischen Standardfärbung – Geschichte, Chemie, Durchführung, Artefakte und Problemlösungen/<i>Pappenheim</i> Stain: Description of a hematological standard stain – history, chemistry, procedure, artifacts and problem solutions

Binder, Thomas R.; Diem, Heinz; Fuchs, Roland J.; Gutensohn, K.; Nebe, Thomas

doi:10.1515/labmed-2012-0027

Cited by 14 publications

(5 citation statements)

References 6 publications

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“…The peripheral blood of healthy people, whose physiological parameters correspond to the normal range, served as a control. The collected peripheral blood streams were fixed in 96% ethyl alcohol and stained with Gimza Mein-Grunwald solutions, according to Pappenheim [ 24 ], and Shiffe reactive according to Feulgen [ 16 ]. The hematological samples were examined using a trinocular microscope (B-293, OptikamB5 Digital Camera, Italy), and Optika Liteview software was used to record the images at a magnification of×400 and ×1000.…”

Section: Methodsmentioning

confidence: 99%

Morphological abnormalities of peripheral blood cells among patients with COVID-19 disease

Karapetyan,

Niazyan,

Shushanyan

et al. 2024

Heliyon

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Section: Methodsmentioning

confidence: 99%

Morphological abnormalities of peripheral blood cells among patients with COVID-19 disease

Karapetyan,

Niazyan,

Shushanyan

et al. 2024

Heliyon

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“…Settings of picture quality were controlled with the assistance of the software program ZEN 3.0 blue edition.Ink (Carl Zeiss Meditec AG) and the captured images were subsequently cropped using Microsoft ® Paint 3D program (Microsoft Corporation, CA, USA), so that the images had a uniform size of 1500 × 1500 pixels and the cells were located in the center of the image. The blood smears were stained using standard hematological staining in accordance with Pappenheim ( 22 ), consisting of May-Grünwald and Giemsa staining solutions (manufactured by Merck KGaA, Darmstadt, Germany). To encourage the students to make internal medical interrelationships and to avoid monotonous working on blood cells, the preparation of the dWBC was supplemented by further tasks to complete a clinical case with a hematological focus.…”

Section: Methodsmentioning

confidence: 99%

Teaching clinical hematology and leukocyte differentiation in veterinary medicine using virtual patients

Marahrens,

Wagener,

Schaper

et al. 2023

Front. Vet. Sci.

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Due to contact restrictions imposed because of the COVID-19 pandemic, we created a novel digital course on the Moodle learning platform for winter term in 2020. In the clinical pathology course (CPC) with hematological content, third-year students were able to work independently on 10 extra digital cases of internal medicine involving eight different animal species as a compensation for the reduction in traditional microscopy exercises. Each case presented was initiated using an anamnesis, also the participants to generate a differential blood count based on digitized leukocytes, previously been photographed using a microscope camera. The cases were successive and increased in complexity, for example through the increase in the number of different cell types to be differentiated. The participants had the opportunity to evaluate the course through a final module to rate user-friendliness and acceptance. The total results of the participants in 2021 were analyzed descriptively, focusing on success rates, time spent on the tasks, and number of attempts. A total of 237 (= 96%) of 247 students completed all cases, each assessing 1033 photographed blood cells in sum. The mean processing time was 22.48 min for a differentiation and the students spent an average of 1.48 attempts on it. A voluntary feedback form was completed by 192 (= 78%) students, with more than 95% rating the course positively in 12 evaluation questions, and 29 of 33 comments (= 87.88%) providing positive statements in a comment box. Suggestions for improvement primarily included more explanations on erythrocyte morphologies, followed by adjusting the difficulty level and improving the presentational set-up. Slight improvements in results, time spent on processing the tasks, and the number of attempts indicated an achievement of routine and confidence during the course and were associated with an increase of competency. The positive feedback showed a high acceptance of the digital format and students evaluated the course as improving the quality of teaching when combined with practical exercises.

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“…One drop of blood was pipetted onto a slide and stained with Pappenheimer panoptic staining according to an established protocol. [ 46 ] The white blood was differentiated manually in blood smear images blinded to the source, and the leukocytes were divided into individual types. For this purpose, 100 leukocytes were counted in each blood smear analysis.…”

Section: Methodsmentioning

confidence: 99%

Biodegradation and Immunological Parameters of Polyurethane‐Based Tissue Adhesive in Arterial Microvascular Anastomoses—A Long‐Term In Vivo Study

Modabber

Winnand

Goloborodko

et al. 2022

Macromolecular Bioscience

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In microsurgical anastomosis, non‐synthetic fibrin‐based adhesives have predominantly shown superior properties to synthetic cyanoacrylates, but they have hardly any clinical application. This study aims to investigate the local and systemic effects of synthetically produced biodegradable adhesive VIVO when used in microsurgical anastomosis. VIVO is used in two different anastomosis procedures in the common carotid artery in a rat model: VIVO in addition to a temporary catheter (VIVO TC) and VIVO with a custom‐shaped memory nitinol stent (VIVO SM). Conventionally sutured anastomoses serve as controls (C). Tissue response is assessed by in vivo fluorescence imaging and histological examination. The systemic effects of biodegradation are measured using hematologic parameters and serum levels of transaminase activity and lactate dehydrogenase. Finally, the degree of local adhesion of the different anastomotic procedures is evaluated. Fluorescence imaging shows reduced inflammatory blood flow in the VIVO TC group. Histological analysis of the anastomosed vessels also reveals significantly more inflammation in C than in the two adhesive groups. The severity of VIVO adhesions proves acceptable, and no histotoxic effects of VIVO are detected. The data demonstrate that the synthetic tissue adhesive VIVO is a reliable and—compared to sutures—tissue‐friendly adhesive for microsurgical anastomoses.

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Pappenheim-Färbung: Beschreibung einer hämatologischen Standardfärbung – Geschichte, Chemie, Durchführung, Artefakte und Problemlösungen/Pappenheim Stain: Description of a hematological standard stain – history, chemistry, procedure, artifacts and problem solutions

Abstract: ZusammenfassungVor genau 100 Jahren hat

Cited by 14 publications

References 6 publications

Morphological abnormalities of peripheral blood cells among patients with COVID-19 disease

Morphological abnormalities of peripheral blood cells among patients with COVID-19 disease

Teaching clinical hematology and leukocyte differentiation in veterinary medicine using virtual patients

Biodegradation and Immunological Parameters of Polyurethane‐Based Tissue Adhesive in Arterial Microvascular Anastomoses—A Long‐Term In Vivo Study

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