<i>Paracoccidioides</i>
            Genomes Reflect High Levels of Species Divergence and Little Interspecific Gene Flow

Mavengere, Heidi; Mattox, Kathleen; Teixeira, Marcus M.; Sepúlveda, Victoria E.; Gómez, Oscar Mauricio Hernández; Hernández, Orville; McEwen, Juan G.; Matute, Daniel R.

doi:10.1128/mbio.01999-20

Cited by 22 publications

(20 citation statements)

References 79 publications

(122 reference statements)

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“…More recently, the genome of 77 strains representing the five species of Paracoccidioides was sequenced using short-read Illumina technology and used for evolutionary analysis. The results reinforced that all Paracoccidioides species are reciprocally monophyletic, and gene flow (or hybridization) between species is infrequent [25,26]. Today, five structural draft genomes of Paracoccidioides isolates (Pb18, Pb03, Pb01, PbCnh and Pb300) have been sequenced, assembled, annotated and deposited in genomic databases.…”

Section: From the First Description Of The Disease To Functional Genomics-112 Years Of Gathered Knowledge A Brazilian Middle West Perspecmentioning

confidence: 76%

One Century of Study: What We Learned about Paracoccidioides and How This Pathogen Contributed to Advances in Antifungal Therapy

Kioshima

Mendonca

Teixeira

et al. 2021

JoF

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Paracoccidioidomycosis (PCM) is a notable fungal infection restricted to Latin America. Since the first description of the disease by Lutz up to the present day, Brazilian researchers have contributed to the understanding of the life cycle of this pathogen and provided the possibility of new targets for antifungal therapy based on the structural and functional genomics of Paracoccidioides. In this context, in silico approaches have selected molecules that act on specific targets, such as the thioredoxin system, with promising antifungal activity against Paracoccidioides. Some of these are already in advanced development stages. In addition, the application of nanostructured systems has addressed issues related to the high toxicity of conventional PCM therapy. Thus, the contribution of molecular biology and biotechnology to the advances achieved is unquestionable. However, it is still necessary to transcend the boundaries of synthetic chemistry, pharmaco-technics, and pharmacodynamics, aiming to turn promising molecules into newly available drugs for the treatment of fungal diseases.

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Section: From the First Description Of The Disease To Functional Genomics-112 Years Of Gathered Knowledge A Brazilian Middle West Perspecmentioning

confidence: 76%

One Century of Study: What We Learned about Paracoccidioides and How This Pathogen Contributed to Advances in Antifungal Therapy

Kioshima

Mendonca

Teixeira

et al. 2021

JoF

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“…The analysis of the GP43 and ARF sequences allowed to observe that the majority of the isolates in the present study presented complete identity or a few nucleotides difference from isolates of S1b type. The predominance of S1b lineage helps explain the high genetic variability found that even with a small number of isolates it was higher than that observed for the isolates from other Brazilian states and Latin American countries where the PCM is endemic [ 11 , 52 , 55 ].…”

Section: Discussionmentioning

confidence: 98%

“…In accordance with the taxonomic evolution, S1/ P . brasiliensis was proposed to be constituted by two lineages, S1a and S1b [ 11 ], which was later endorsed by other authors [ 18 , 52 , 55 ]. The S1b lineage was associated with most isolates of Paracoccidioides spp.…”

Section: Discussionmentioning

confidence: 99%

“…americana exhibited a lower frequency of introgressions and genetic exchanges when compared to other species of the P . brasiliensis complex [ 55 ]. Isolates 769 and 133 clustered polyphyletically with PS2/ P .…”

Section: Discussionmentioning

confidence: 99%

“…[50], P. lutzii [58,67] and S1/P. brasiliensis [11,55], while ED01 was characterized as P. lutzii [11,58]. Similarly, AT22 was found in EPM104 and 5979 isolates from clinical origin from Parana ´and Goia ´s, respectively.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 96%

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Molecular epidemiology of Paracoccidiodes spp. recovered from patients with paracoccidioidomycosis in a teaching hospital from Minas Gerais State of Brazil

Andrade-Silva

Paes

et al. 2021

PLoS Negl Trop Dis

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Introduction Paracoccidioidomycosis (PCM) is caused by several species of the Paracoccidioides genus which can be differentiated by interspecific genetic variations, morphology and geographic distribution. Intraspecific variability correlation with clinical and epidemiological aspects of these species still remains unclear. This study aimed to sequence the loci GP43, exon 2 and ARF of 23 clinical isolates of Paracoccidioides spp. from patients in the Southeast Region of Brazil. Methodology and main findings GenBank was used to compare the present (23) with previous described sequences (151) that included ARF and GP43. It was identified a high polymorphism rate among the 23 isolates in comparison to the other 151. Among the isolates, 22 (95,66%) were S1/P. brasiliensis and 1 (4,34%) was identified as PS2/P. americana. A total of 45 haplotypes were found as follows: 19 from S1/P. brasiliensis (13 from the present study), 15 from P. lutzii, 6 from PS2/P. americana (1 from the present study), 3 from PS3/P. restrepiensis and 2 from PS4/P. venezuelensis. Moreover, exclusive haplotypes according to clinical origin and geographical area were found. S1/P. brasiliensis (HD = 0,655 and K = 4,613) and P. lutzii (HD = 0,649 and K = 2,906) presented the highest rate of polymorphism among all species, from which 12 isolates of the present study were clustered within S1b/P. brasiliensis. The GP43 locus showed a higher variability and was found to be the main reason for the species differentiation. Conclusions The results herein decribed show a high intraspecific genetic variability among S1/P. brasiliensis isolates and confirm the predominance of this species in the Southeast region of Brazil. The finding of exclusive haplotypes according to clinical origin and geographical area would suggest correlation between the molecular profile with the clinical form and geographic origin of patients with PCM.

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Histoplasma , Blastomyces , Coccidioides , Paracoccidioides and Other Dimorphic Fungi Causing Systemic Mycoses

Thompson,

Gomez

2023

ClinMicroNow

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The dimorphic fungi causing systemic disease belong to the class Eurotiomycetes , order Onygenales . This chapter covers the dimorphic members of the families Onygenaceae and Ajellomycetaceae . Ajellomycetaceae contains the species Ajellomyces dermatitidis , A. capsulatum , A. duboisii , and A. crescens , the anamorphs of which have been placed in the genera Blastomyces and Histoplasma . DNA sequence data place Paracoccidioides in the family Ajellomycetaceae as well. However, it is important to appreciate that tissue‐form cells of Histoplasma and Blastomyces can appear similar to each other as well as to yeast cells of various Candida species, Cryptococcus neoformans , Cryptococcus gattii , and Talaromyces marneffei and to endospores of Coccidioides species.

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Paracoccidioides Genomes Reflect High Levels of Species Divergence and Little Interspecific Gene Flow

Abstract: Paracoccidioides is the causal agent of a systemic mycosis in Latin America. Most of the inference of the evolutionary history of Paracoccidioides has used only a few molecular markers.

Cited by 22 publications

References 79 publications

One Century of Study: What We Learned about Paracoccidioides and How This Pathogen Contributed to Advances in Antifungal Therapy

One Century of Study: What We Learned about Paracoccidioides and How This Pathogen Contributed to Advances in Antifungal Therapy

Molecular epidemiology of Paracoccidiodes spp. recovered from patients with paracoccidioidomycosis in a teaching hospital from Minas Gerais State of Brazil

Histoplasma , Blastomyces , Coccidioides , Paracoccidioides and Other Dimorphic Fungi Causing Systemic Mycoses

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