<i>Paratylenchus neoprojectus</i> n. sp. (Paratylenchinae: Nematoda) from alfalfa fields in Alberta, Canada

Wu, Liang-Yu; Hawn, E. J.

doi:10.1139/z75-217

Cited by 7 publications

(4 citation statements)

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“…The newly obtained sequences of the D2-D3 expansion segments of the 28S rRNA, the ITS of the rRNA, and partial COI mtDNA genes were aligned using ClustalX 1.83 [91] with corresponding published gene sequences [13,14,23,24,26,30,[65][66][67]69,71,80,[92][93][94][95][96][97]. Outgroup taxa for each dataset were chosen based on previously published data [30].…”

Section: Phylogenetic and Sequence Analysismentioning

confidence: 99%

Morphological and Molecular Diversity among Pin Nematodes of the Genus Paratylenchus (Nematoda: Paratylenchidae) from Florida and Other Localities and Molecular Phylogeny of the Genus

Álvarez-Ortega

Subbotin

Wang

et al. 2023

Plants

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Pin nematodes (Paratylenchus spp.) are root parasites found worldwide. They have different life cycles and feeding habits and can damage a wide range of plants. A remarkable diversity of pin nematode species was found in soil samples from Florida and other states of the USA, Canada, and Spain. Using integrative taxonomy, two new species (Paratylenchus hawaiiensis sp. n. and P. roboris sp. n.), six valid species (Paratylenchulus acti, P. aquaticus, P. goldeni, P. paralatescens, P. minutus (=P. shenzhenensis syn. n.), and P. straeleni), and two undescribed species were identified from Florida; P. goldeni, P. hamatus, P. hamicaudatus, P. holdemani, and P. pedrami were found in California, P. minutus in Hawaii, P. goldeni in Oregon and Washington, and one new species, Paratylenchus borealis sp. n., in Alaska. Outside the USA, Paratylenchus projectus was detected in samples from Canada and Spain as well as P. holdemani and Paratylenchus sp. from Spain. The pin nematode species from Belgium and Russia identified in former studies as Paratylenchus sp. F was herein described as a new species with the name of P. borealis sp. n., using a population from Alaska. Previously reported molecular type A of P. aquaticus from Hawaii was reclassified as P. hawaiiensis sp. n., using a population from Florida. Paratylenchus roboris sp. n. from Florida has obese sedentary females with a stylet 63–71 µm long. The results of the molecular analysis of P. shenzhenensis from Florida and China indicated that it was conspecific with P. minutus from Hawaii and considered here as its junior synonym. New 26 D2–D3 expansion segments of 28S rRNA, 17 ITS rRNA, and 20 COI gene sequences were obtained in this study. Phylogenetic relationships of Paratylenchus are reconstructed using the D2–D3 of 28S rRNA, ITS rRNA, and COI gene sequences. Congruence of molecular and morphological evolution and species identification problems are discussed. Obese females were found in two major clades of Paratylenchus. The problem of reference materials is discussed, and it is proposed to make more efforts to collect topotype materials of known Paratylenchus species for molecular study.

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Section: Phylogenetic and Sequence Analysismentioning

confidence: 99%

Morphological and Molecular Diversity among Pin Nematodes of the Genus Paratylenchus (Nematoda: Paratylenchidae) from Florida and Other Localities and Molecular Phylogeny of the Genus

Álvarez-Ortega

Subbotin

Wang

et al. 2023

Plants

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“…As several short stylet pin nematodes species are considered to be plant-pathogenic [22], we performed morphological/morphometrical and molecular studies on these Paratylenchus populations and identified them as P. neoprojectus Wu and Hawn [26], P. tateae Wu and Townshend [27], and a new Paratylenchus sp. that we named P. enigmaticus sp.…”

Section: Of 25mentioning

confidence: 99%

“…The results of this study will aid in distinguishing pathogenic forms from non-pathogenic species, and our findings will be useful in future field experiments to determine the impact of these PPN on potato production. (Figures 1 and 2; Table 1) [26]. Body slender, ventrally arcuate with a bend in the middle of the body when heat relaxed; cuticle finely annulated; lateral field equidistant with four distinct lines; lip region rounded narrow, with anterior end flattened, continuous with the rest of the body; labial framework sclerotization weak; pharyngeal region typical paratylenchoid type; stylet rigid, straight; rounded stylet knobs; dorsal pharyngeal gland opening 5.0-6.0 µm behind stylet knobs; median pharyngeal bulb large elongate, bearing distinct large valves; isthmus short slender, surrounded by nerve ring; basal bulb pyriform, pharyngeal-intestinal valve bilobed; excretory pore situated at the level or middle of pharyngeal basal bulb.…”

Section: Of 25mentioning

confidence: 99%

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Species Diversity of Pin Nematodes (Paratylenchus spp.) from Potato Growing Regions of Southern Alberta, Canada

Maria

Yevtushenko

Palomares-Rius

et al. 2021

Plants

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Pin nematodes (Paratylenchus spp.) are polyphagous parasitic species with a wide host range and geographical distribution; their diversity is unknown in the potato growing region of Alberta, Canada. The present study aims to provide morphological and molecular characterization of three pin nematode species, namely P. neoprojectus, P. tateae, and a new species, Paratylenchus enigmaticus sp. nov. All of them were recovered from the potato growing region of southern Alberta. The nematodes were isolated using the sieving and flotation-centrifugation method, and their morphology was assessed by light microscopy. Molecular characterization was performed using partial 18S, D2–D3 expansion domains of the 28S and ITS ribosomal genes. This study is the first report of molecular characterization of P. tateae and P. neoprojectus, being new records from southern Alberta, and two Spanish populations of P. tateae comprising the first report of this species in Europe. The phylogenetic analysis of the 18S, D2–D3 expansion domains of the 28S and ITS ribosomal DNA regions underscores the importance of using molecular data for accurate species identification and clarifies the status of P. nanus type B and P. sheri. Moreover, our findings will be useful to determine the impact of pin nematodes on potato production in future field research.

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Paratylenchus conicephalus sp. n. from Iran with notes on P. similis Khan, Prasad & Mathur, 1967 (Nematoda: Paratylenchinae)

Karegar

Tiedt

Berg³

et al. 2011

Nematol

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During nematode surveys in Iran two Paratylenchus populations were found. Paratylenchus conicephalus sp. n. is described and figured from the rhizosphere of Prangos ferulacea (L.) Lindl. and is characterised by having a 365-501 μm long body, stylet 29.5-35 μm long, a conical lip region with four or five indistinct annuli, large submedian lobes and four large flaps around the stoma opening, these structures appearing as a small cap-like structure when viewed laterally with the light microscope, four lines in the lateral field, and a sharply pointed tail 25.5-46 μm long. Paratylenchus similis is described and figured from the rhizosphere of Artemisia aucheri Boiss. and compared with the various descriptions of this species.

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Paratylenchus neoprojectus n. sp. (Paratylenchinae: Nematoda) from alfalfa fields in Alberta, Canada

Cited by 7 publications

References 1 publication

Morphological and Molecular Diversity among Pin Nematodes of the Genus Paratylenchus (Nematoda: Paratylenchidae) from Florida and Other Localities and Molecular Phylogeny of the Genus

Morphological and Molecular Diversity among Pin Nematodes of the Genus Paratylenchus (Nematoda: Paratylenchidae) from Florida and Other Localities and Molecular Phylogeny of the Genus

Species Diversity of Pin Nematodes (Paratylenchus spp.) from Potato Growing Regions of Southern Alberta, Canada

Paratylenchus conicephalus sp. n. from Iran with notes on P. similis Khan, Prasad & Mathur, 1967 (Nematoda: Paratylenchinae)

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