<i>Pasteurella</i>

Mutters, Reinier; Bisgaard, Magne

doi:10.1002/9781118960608.gbm01201

“…Each swab was plated on tryptic soy yeast extract agar (Difco-BBL) supplemented by 5% of defibrinated sheep blood and incubated at 37 °C for 24 h. From each plate, one to five colonies with morphology suggestive of P. multocida were selected. Colonies were identified using standard biochemical procedures, including the production of catalase, oxidase, and indol, urease activity, the production of ornithine decarboxylase, carbohydrate fermentation ( Mutters et al , 1989 ) and PCR for the detection of the species-specific gene fragment kmt ( Townsend et al , 1998 ).…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial resistance and virulence gene profiles in P. multocida strains isolated from cats

Ferreira¹,

Felizardo²,

Gobbi³

et al. 2015

Braz. J. Microbiol.

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Cats are often described as carriers of Pasteurella multocida in their oral microbiota. This agent is thought to cause pneumonia, conjunctivitis, rhinitis, gingivostomatitis, abscess and osteonecrosis in cats. Human infection with P. multocida has been described in several cases affecting cat owners or after cat bites. In Brazil, the cat population is approximately 21 million animals and is increasing, but there are no studies of the presence of P. multocida in the feline population or of human cases of infection associated with cats. In this study, one hundred and ninety-one healthy cats from owners and shelters in São Paulo State, Brazil, were evaluated for the presence of P. multocida in their oral cavities. Twenty animals were positive for P. multocida , and forty-one strains were selected and characterized by means of biochemical tests and PCR. The P. multocida strains were tested for capsular type, virulence genes and resistance profile. A total of 75.6% (31/41) of isolates belonged to capsular type A, and 24.4% (10/41) of the isolates were untypeable. None of the strains harboured toxA, tbpA or pfhA genes. The frequencies of the other genes tested were variable, and the data generated were used to build a dendrogram showing the relatedness of strains, which were clustered according to origin. The most common resistance profile observed was against sulfizoxazole and trimethoprim-sulphamethoxazole.

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“…[Pasteurella] pneumotropica biotypes Jawetz and Heyl and [Actinobacillus] muris are the most prevalent Pasteurellaceae species isolated from laboratory mouse [ 5 ]. Although described several decades ago, these species are expected to undergo taxonomic changes soon, since they have not been formally classified under genera due to insufficient knowledge regarding their taxonomy [ 6 ]. [P . ]…”

Section: Introductionmentioning

confidence: 99%

Characterization of Biofilm Formation in [Pasteurella] pneumotropica and [Actinobacillus] muris Isolates of Mouse Origin

Sager

¹

,

Benten

²

,

Engelhardt

³

et al. 2015

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[Pasteurella] pneumotropica biotypes Jawetz and Heyl and [Actinobacillus] muris are the most prevalent Pasteurellaceae species isolated from laboratory mouse. However, mechanisms contributing to their high prevalence such as the ability to form biofilms have not been studied yet. In the present investigation we analyze if these bacterial species can produce biofilms in vitro and investigate whether proteins, extracellular DNA and polysaccharides are involved in the biofilm formation and structure by inhibition and dispersal assays using proteinase K, DNase I and sodium periodate. Finally, the capacity of the biofilms to confer resistance to antibiotics is examined. We demonstrate that both [P.] pneumotropica biotypes but not [A.] muris are able to form robust biofilms in vitro, a phenotype which is widely spread among the field isolates. The biofilm inhibition and dispersal assays by proteinase and DNase lead to a strong inhibition in biofilm formation when added at the initiation of the biofilm formation and dispersed pre-formed [P.] pneumotropica biofilms, revealing thus that proteins and extracellular DNA are essential in biofilm formation and structure. Sodium periodate inhibited the bacterial growth when added at the beginning of the biofilm formation assay, making difficult the assessment of the role of β-1,6-linked polysaccharides in the biofilm formation, and had a biofilm stimulating effect when added on pre-established mature biofilms of [P.] pneumotropica biotype Heyl and a majority of [P.] pneumotropica biotype Jawetz strains, suggesting that the presence of β-1,6-linked polysaccharides on the bacterial surface might attenuate the biofilm production. Conversely, no effect or a decrease in the biofilm quantity was observed by biofilm dispersal using sodium periodate on further biotype Jawetz isolates, suggesting that polysaccharides might be incorporated in the biofilm structure. We additionally show that [P.] pneumotropica cells enclosed in biofilms were less sensitive to treatment with amoxicillin and enrofloxacin than planktonic bacteria. Taken together, these findings provide a first step in understanding of the biofilm mechanisms in [P.] pneumotropica, which might contribute to elucidation of colonization and pathogenesis mechanisms for these obligate inhabitants of the mouse mucosa.

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“…In the present paper, we compare these taxa with taxon 35, a previously unreported group of members of the family Pasteurellaceae from dogs, to improve our understanding and the diagnosis of these organisms. The insertion of the genus name in brackets signifies misclassification of the species with respect to the type species of the genus ( Haemophilus influenzae ) [7, 8]. Taxon 35 of Bisgaard has remained unpublished, however, it was described in a conference proceeding report [9].…”

Section: Full-textmentioning

confidence: 99%

Reclassification of [Haemophilus] haemoglobinophilus as Canicola haemoglobinophilus gen. nov., comb. nov. including Bisgaard taxon 35

Kuhnert

¹

,

Foster²,

Bisgaard

³

2021

International Journal of Systematic and Evolutionary Microbiology

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[ Haemophilus ] haemoglobinophilus and the unpublished Bisgaard taxon 35 are associated with respiratory and urogenital tract infections in dogs. A total of 21 strains including the type strain of [ Haemophilus ] haemoglobinophilus were included in the investigation. Strains of [ Haemophilus ] haemoglobinophilus and taxon 35 formed a monophyletic group demonstrating at least 97.8 and 96.5% similarities within the group based upon 16S rRNA and rpoB gene sequence comparisons, respectively. Glaesserella australis was the most closely related species to [ Haemophilus ] haemoglobinophilus and taxon 35 with 96.1 % 16S rRNA gene sequence similarity which is slightly higher than the 95 % separating most genera of the family Pasteurellaceae . However, the conserved protein sequence phylogeny documented a unique position of [ Haemophilus ] haemoglobinophilus with only 81 % identity to the most closely related species, genomospecies 1 of the genus Rodentibacter which is lower than the 85 % separating most genera of the family Pasteurellaceae . The conserved protein sequence identity to Haemophilus influenzae , the type species of the genus, was 77%, demonstrating that [ Haemophilus ] haemoglobinophilus is not properly classified as a member of the genus Haemophilus . On the basis of the phylogenetic comparisons, the taxa [ Haemophilus ] haemoglobinophilus and taxon 35 are proposed to be included with a novel genus Canicola with one species, Canicola haemoglobinophilus which is reclassified from [ Haemophilus ] haemoglobinophilus. Phenotypic characters obtained with isolates genetically approved to represent Canicola haemoglobinophilus were in accordance with those of the members of the family Pasteurellaceae, and the novel genus can be separated from most of the existing genera by a positive catalase reaction, lack of V-factor requirement for growth, lack of haemolysis of blood agar and negative Voges–Proskauer and urease tests. The novel genus cannot be separated by biochemical and physiological characteristics alone from the genera Aggregatibacter , Avibacterium , Frederiksenia and Spirabiliibacterium . However, MALDI-TOF mass spectroscopy and also RpoB amino acid signatures allowed a clear separation from these taxa, supporting the existence of a novel genus. The DNA G+C content is 37.0–37.8 mol% for the genus, based on the whole genomic sequences. The type strain of Canicola haemoglobinophilus is CCUG 3714T (=ATCC 19416T=NCTC 1659T) isolated in 1901 from the prepuce of a dog in Germany.

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Pasteurella

Cited by 4 publications

References 108 publications

Antimicrobial resistance and virulence gene profiles in P. multocida strains isolated from cats

Antimicrobial resistance and virulence gene profiles in P. multocida strains isolated from cats

Characterization of Biofilm Formation in [Pasteurella] pneumotropica and [Actinobacillus] muris Isolates of Mouse Origin

Reclassification of [Haemophilus] haemoglobinophilus as Canicola haemoglobinophilus gen. nov., comb. nov. including Bisgaard taxon 35

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