<i>Pasteurella</i>and<i>Mannheimia</i>

Boyce, John D.; Lo, Reggie Y.C.; Wilkie, Ian; Adler, Ben

doi:10.1002/9780470344903.ch20

Cited by 13 publications

(21 citation statements)

References 154 publications

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“…The nasal cavity is considered the natural habitat of P. multocida in different species, including the rabbit [10]; however, neither the normal ultrastructure of this region nor the pathologic changes during natural infection with P. multocida have been described in rabbits. …”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Comparison of the Nasal Epithelia of Healthy and Naturally Affected Rabbits withPasteurella multocidaA

Esquinas

Botero

Patiño

et al. 2013

Veterinary Medicine International

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An ultrastructural comparison between the nasal cavities of healthy rabbits and those suffering from two forms of spontaneous infection with Pasteurella multocida was undertaken. Twelve commercially produced rabbits of different ages and respiratory health status were divided into four groups: healthy from 0 to 21 days (G1, n = 2); healthy from 23 to 49 days (G2, n = 2); healthy from 51 to 69 days (G3, n = 2); diseased rabbits with septicemia and the rhinitic form of P. multocida infection (G4, n = 3). The main ultrastructural changes observed were a widening of the interepithelial spaces, increased activity and number of goblet cells, the formation of two types of vacuoles in epithelial cells, the degranulation and migration of heterophils between the epithelial cells, and the association of this migration with some of the other changes. No bacteria were observed adhering to the epithelium, and very few were observed free in the mucus. Scant inter-epithelial spaces were found in healthy rabbits, but they were not as large and numerous as those found in diseased animals. We discuss the origin and meaning of these changes but, we focus on the significance of the inter-epithelial spaces and goblet cells for the defense of the upper respiratory airways against the bacterium and its lipopolysaccharide.

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Section: Introductionmentioning

confidence: 99%

Ultrastructural Comparison of the Nasal Epithelia of Healthy and Naturally Affected Rabbits withPasteurella multocidaA

Esquinas

Botero

Patiño

et al. 2013

Veterinary Medicine International

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“…At present commercial vaccines are available for a range of diseases caused by P. multocida [9]. Bacterins are widely used to vaccinate against fowl cholera, hemorrhagic septicemia, and atrophic rhinitis [10]–[12].…”

Section: Introductionmentioning

confidence: 99%

Screening of 71 P. multocida Proteins for Protective Efficacy in a Fowl Cholera Infection Model and Characterization of the Protective Antigen PlpE

et al. 2012

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Background There is a strong need for a recombinant subunit vaccine against fowl cholera. We used a reverse vaccinology approach to identify putative secreted or cell surface associated P. multocida proteins that may represent potential vaccine candidate antigens. Principal Findings A high-throughput cloning and expression protocol was used to express and purify 71 recombinant proteins for vaccine trials. Of the 71 proteins tested, only one, PlpE in denatured insoluble form, protected chickens against fowl cholera challenge. PlpE also elicited comparable levels of protection in mice. PlpE was localized by immunofluorescence to the bacterial cell surface, consistent with its ability to elicit a protective immune response. To explore the role of PlpE during infection and immunity, a plpE mutant was generated. The plpE mutant strain retained full virulence for mice. Conclusion These studies show that PlpE is a surface exposed protein and was the only protein of 71 tested that was able to elicit a protective immune response. However, PlpE is not an essential virulence factor. This is the first report of a denatured recombinant protein stimulating protection against fowl cholera.

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“…Fowl cholera is an economically important disease of poultry that occurs in most countries. The pathogenesis of infection is not well understood at the molecular level, but initial colonization is thought to occur via the host mucosa and may proceed to a rapid and severe systemic disease that is generally fatal (4). Most fowl cholera outbreaks in the poultry industry are associated with strains belonging to serogroup A or F (4).…”

mentioning

confidence: 99%

“…It is also responsible for a range of diseases in mammals, including bovine hemorrhagic septicemia and swine atrophic rhinitis (4). Serological classification of P. multocida strains is based primarily on the capsular type, which divides strains into serogroups A, B, D, E, and F (7,26), and secondly on lipopolysaccharide (LPS) antigens that allow further classification into 16 Heddleston serotypes (16).…”

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confidence: 99%

Identification of Novel Glycosyltransferases Required for Assembly of the Pasteurella multocida A:1 Lipopolysaccharide and Their Involvement in Virulence

et al. 2009

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We previously determined the structure of the Pasteurella multocida Heddleston type 1 lipopolysaccharide (LPS) molecule and characterized some of the transferases essential for LPS biosynthesis. We also showed that P. multocida strains expressing truncated LPS display reduced virulence. Here, we have identified all of the remaining glycosyltransferases required for synthesis of the oligosaccharide extension of the P. multocida Heddleston type 1 LPS, including a novel ␣-1,6 glucosyltransferase, a ␤-1,4 glucosyltransferase, a putative bifunctional galactosyltransferase, and two heptosyltransferases. In addition, we identified a novel oligosaccharide extension expressed only in a heptosyltransferase (hptE) mutant background. All of the analyzed mutants expressing LPS with a truncated main oligosaccharide extension displayed reduced virulence, but those expressing LPS with an intact heptose side chain were able to persist for long periods in muscle tissue. The hptC mutant, which expressed LPS with the shortest oligosaccharide extension and no heptose side chain, was unable to persist on the muscle or cause any disease. Furthermore, all of the mutants displayed increased sensitivity to the chicken antimicrobial peptide fowlicidin 1, with mutants expressing highly truncated LPS being the most sensitive.

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PasteurellaandMannheimia

Cited by 13 publications

References 154 publications

Ultrastructural Comparison of the Nasal Epithelia of Healthy and Naturally Affected Rabbits withPasteurella multocidaA

Ultrastructural Comparison of the Nasal Epithelia of Healthy and Naturally Affected Rabbits withPasteurella multocidaA

Screening of 71 P. multocida Proteins for Protective Efficacy in a Fowl Cholera Infection Model and Characterization of the Protective Antigen PlpE

Identification of Novel Glycosyltransferases Required for Assembly of the Pasteurella multocida A:1 Lipopolysaccharide and Their Involvement in Virulence

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