Pediococcus pentosaceus PP04 Ameliorates High-Fat Diet-Induced Hyperlipidemia by Regulating Lipid Metabolism in C57BL/6N Mice

Abstract

In this study, Pediococcus pentococcus PP04 isolated from the Northeast pickled cabbage had good gastrointestinal tolerance and can colonize in the intestine stably. C57BL/6N mice were fed a high-fat diet to build animal models and treated with Pediococcus pentosaceus PP04 to evaluate the antihyperlipidemia effect. After 8 weeks, the indicators of hyperlipidemia, liver injury, and inflammation were measured. The treatment of P. pentosaceus PP04 reduced the gain of total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), free fatty acids (FFAs), leptin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), lipopolysaccharides (LPS), and tumor necrosis factor-α (TNF-α) significantly. The western blotting results suggested P. pentosaceus PP04 ameliorated high-fat diet-induced hyperlipidemia by the AMPK signaling pathway, which stimulated lipolysis via upregulation of PPARα and inhibited lipogenesis by downregulation of SREBP-1c, fatty acid synthase (FAS), and stearoyl-CoA desaturase-1 (SCD1) mainly. Furthermore, P. pentosaceus PP04 improved high-fat diet-induced oxidative stress effectively by triggering the Nrf2/CYP2E1 signaling pathway that enhanced the antioxidant activity including superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px).

Transcriptomic Analysis of Pediococcus pentosaceus Reveals Carbohydrate Metabolic Dynamics Under Lactic Acid Stress

“…P. pentosaceus has long been engineered and applied as bacterial factory to produce valuable substances ( Wu et al, 2004 ; Sun et al, 2019 ). More and more recent studies have illustrated that P. pentosaceus could exert comprehensive host beneficial effects, e.g., antioxidant activities ( Zhang et al, 2020 ), hyperlipidemia relief ( Wang et al, 2020 ), and gut microbiota restoration ( Hao et al, 2021 ). Moreover, the combination between beneficial bacteria (probiotic) and fermentable non-digestible dietary carbohydrate (prebiotic) could manifest synergistic health improvement effects, thus has been attracted much attention in novel functional food manufacturing ( Swanson et al, 2020 ).…”

Pediococcus pentosaceus: Screening and Application as Probiotics in Food Processing

“…Huang et al, (2020) found that P. pentosaceus B49 alleviated oxidative stress in constipated mice by reducing serum malondialdehyde (MDA) levels. Wang et al (2020) found P. pentosaceus PP04 effectively improved oxidative stress induced by high-fat diet by activating Nrf2/CYP2E1 signaling pathway. The signal pathway can be enhanced superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) and antioxidant activity.…”

“…of the mouse fed LAB (live bacteria or cell extracts), including superoxide dismutase (SOD) activity, glutathione peroxidase (GSH-Px) activity, malondialdehyde (MDA) content, protein amino-compound content, serum endotoxin, structural changes in gut microbial community, were measured and compared with those from the murine oxidative damage model. The significant difference was taken as a reference to evaluate the ability of LAB to intervene oxidative stress in vivo ( Wang et al, 2020 ).…”

“…They found that there was a significant decrease of total cholesterol, low-density fatty acids, and alanine aminotransferase in serum, and total cholesterol in liver, along with a significant increase of free cholic acid content in feces. Wang et al (2020) fed C57BL/6 N mice with high-fat diet and treated with P. pentosaceus PP04. The results showed that the weight gain of total cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) in mice was significantly reduced, and similar phenomenon was also found ( Zhao et al, 2012 ).…”

Screening and Identification of Latilactobacillus curvatus Z12 From Rumen Fluid of an Adult Female Sika Deer as a Potential Probiotic for Feed Additives

“…The method of Wang et al ( 28 ) was used to determine autoaggregation, with some modifications. In brief, the isolated strains were cultured in MRS broth at 37°C for 18–24 h (10 9 CFU/mL).…”