Perkinsus marinus in bioreactor: growth and a cost-reduced growth medium
Caitlin Murphy,
José A Fernández Robledo,
G Peter van Walsum
Abstract:Perkinsus marinus (Perkinsea) is an osmotrophic facultative intracellular marine protozoan responsible for ‘Dermo’ disease in the eastern oyster, Crassostrea virginica. In 1993, three laboratories developed the in vitro culture of P. marinus in the absence of host cells. Two of the culture media and conditions went through several optimization reiterations, with one becoming the standard for the small-scale growth of Perkinsus species. Compared to most intracellular protozoan parasites, the availability of P. … Show more
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