Pestalotiopsis revisited

Abstract

Species of Pestalotiopsis occur commonly as plant pathogens, and represent a fungal group known to produce a wide range of chemically novel, diverse metabolites. In the present study, we investigated 91 Pestalotiopsis isolates from the CBS-KNAW Fungal Biodiversity Centre (CBS) culture collection. The phylogeny of the Amphisphaeriaceae was constructed based on analysis of 28S nrRNA gene (LSU) sequence data, and taxonomic changes are proposed to reflect more natural groupings. We combined morphological and DNA data, and segregated two novel genera from Pestalotiopsis, namely Neopestalotiopsis and Pseudopestalotiopsis. The three genera are easily distinguishable on the basis of their conidiogenous cells and colour of their median conidial cells. We coupled morphological and combined sequence data of internal transcribed spacer (ITS), partial β-tubulin (TUB) and partial translation elongation factor 1-alpha (TEF) gene regions, which revealed 30 clades in Neopestalotiopsis and 43 clades in Pestalotiopsis. Based on these data, 11 new species are introduced in Neopestalotiopsis, 24 in Pestalotiopsis, and two in Pseudopestalotiopsis. Several new combinations are proposed to emend monophyly of Neopestalotiopsis, Pestalotiopsis and Pseudopestalotiopsis.

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“…In the review of the genus Pestalotiopsis, Maharachchikumbura et al (2014) made a phylogenetic reconstruction of the Amphisphaeriaceae based on analysis of LSU of the rRNA sequence data. Furthermore, two novel genera were segregated from Pestalotiopsis, namely Neopestalotiopsis and Pseudopestalotiopsis based on combined morphological and DNA data.…”

“…Maharachchikumbura et al (2011) suggested that Pestalotiopsis should be adopted instead of Pestalosphaeria because it is an older and more common name. The genus Neopestalotiopsis was introduced by Maharachchikumbura et al (2014) as sexual morph not observed. The new species presently described is referred to as Neopestalotiopsis pernambucana and it is the first species of the genus encountered with the sexual morph.…”

“…The sexual morph of Pestalotiopsis has not been reported in Brazil. The genus Neopestalotiopsis was segregated from Pestalotiopsis by Maharachchikumbura et al (2014) based on phylogenetic analysis and morphological differences, such as versicolorous median cells and conidiophores indistinct, often reduced to conidiogenous cells, and according to Index Fungorum currently has 25 recognized species (CABI, 2016).…”

Towards incorporating asexual fungi in a natural classification: checklist and notes 2012–2016

“…et al 2014Maharachchikumbura et al (2014 introduced this genus to accommodate several Pestalotiopsis species which did not cluster in Pestalotiopsis sensu stricto. This placement was confirmed by Wijayawardene et al (2016).…”

Endophytic Pestalotiopsis Species From Andaman Islands: A Potential Pancreatic Lipase Inhibitor

“…The microscopic characters were studied using a polarizing optical microscope (Olympus BX-51 P) coupled with charge-coupled device camera and measurements carried out using image J software. At least 30 observations were made per structure [7,8]. The potential solvent extract was separated on silica plates using thin-layer chromatography using solvent hexane:methanol (70:30).…”