<i>Photobacterium damselae</i>subsp.<i>piscicida</i>responds to antimicrobial peptides through phage-shock-protein A (PspA)-related extracytoplasmic stress response system

Tsai, W.-C.; Kuo, Tsun-Yung; Lin, Chun-Yen; Lin, Jianrong; Chen, W.-J.

doi:10.1111/jam.12672

Cited by 7 publications

(7 citation statements)

References 31 publications

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“…Recently, we synthesized a class of cationic AMPs and studied their anti-bacterial activities, as well as their interactions with bacteria [ 25 – 29 ]. We showed that these synthetic AMPs exhibit high cytotoxicity against various cancer cells by inducing apoptosis [ 30 – 32 ].…”

Section: Introductionmentioning

confidence: 99%

A synthetic cationic antimicrobial peptide inhibits inflammatory response and the NLRP3 inflammasome by neutralizing LPS and ATP

Hsieh

et al. 2017

PLoS ONE

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Antimicrobial peptides (AMPs) are one of the most important defense mechanisms against bacterial infections in insects, plants, non-mammalian vertebrates, and mammals. In the present study, a class of synthetic AMPs was evaluated for anti-inflammatory activity. One cationic AMP, GW-A2, demonstrated the ability to inhibit the expression levels of nitric oxide (NO), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in lipopolysaccharide (LPS)-activated macrophages. GW-A2 reduced LPS-induced increases in the phosphorylation of mitogen-activated protein kinase and protein kinase C-α/δ and the activation of NF-κB. GW-A2 also inhibited NLRP3 inflammasome activation induced by LPS and ATP. Furthermore, in the mice injected with LPS, GW-A2 reduced (1) the concentration of IL-1β, IL-6 and TNF-α in the serum; (2) the concentration of TNF-α in the peritoneal lavage; (3) the expression levels of iNOS, COX-2 and NLRP3 in the liver and lung; (4) the infiltration of polymorphonuclear neutrophils in the liver and lung. The underlying mechanisms for the anti-inflammatory activity of GW-A2 were found to be partially due to LPS and ATP neutralization. These results provide insights into how GW-A2 inhibits inflammation and the NLRP3 inflammasome and provide a foundation for the design of rational therapeutics for inflammation-related diseases.

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Section: Introductionmentioning

confidence: 99%

A synthetic cationic antimicrobial peptide inhibits inflammatory response and the NLRP3 inflammasome by neutralizing LPS and ATP

Hsieh

et al. 2017

PLoS ONE

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“…Evidence has suggested that AMPs are of greatest potential to represent such a new type of antibiotics [3][4][5]. We have designed and developed a series of cationic α-helical AMPs with potent activity and selectivity against a broad spectrum of Gram-negative and Gram-positive strains [12][13][14][15][16]. In our previous study, a potent AMP GW-Q6 exhibited potential antimicrobial activity against an MDR strain of Salmonella enterica serovar Choleraesuis [17].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, we have designed and synthesized a series of cationic and amphipathic α-helical AMPs with enhanced activity and selectivity against a broad spectrum of Gram-positive and Gram-negative strains [12]. According to our previous studies, our novel AMPs exhibited potent antibacterial efficacy against several marine pathogenic bacteria [13][14][15][16] and another important bacterial pathogen in pigs, Salmonella enterica serovar Choleraesuis [17]. However, it remains unknown whether AMPs possess antibacterial activity to combat multidrug-resistant (MDR) strains.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antibiofilm Activities of Novel Antimicrobial Peptides against Multidrug-Resistant Enterotoxigenic Escherichia Coli

Hua

et al. 2021

IJMS

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Post-weaning diarrhea due to enterotoxigenic Escherichia coli (ETEC) is a common disease of piglets and causes great economic loss for the swine industry. Over the past few decades, decreasing effectiveness of conventional antibiotics has caused serious problems because of the growing emergence of multidrug-resistant (MDR) pathogens. Various studies have indicated that antimicrobial peptides (AMPs) have potential to serve as an alternative to antibiotics owing to rapid killing action and highly selective toxicity. Our previous studies have shown that AMP GW-Q4 and its derivatives possess effective antibacterial activities against the Gram-negative bacteria. Hence, in the current study, we evaluated the antibacterial efficacy of GW-Q4 and its derivatives against MDR ETEC and their minimal inhibition concentration (MIC) values were determined to be around 2~32 μg/mL. Among them, AMP Q4-15a-1 with the second lowest MIC (4 μg/mL) and the highest minimal hemolysis concentration (MHC, 256 μg/mL), thus showing the greatest selectivity (MHC/MIC = 64) was selected for further investigations. Moreover, Q4-15a-1 showed dose-dependent bactericidal activity against MDR ETEC in time–kill curve assays. According to the cellular localization and membrane integrity analyses using confocal microscopy, Q4-15a-1 can rapidly interact with the bacterial surface, disrupt the membrane and enter cytosol in less than 30 min. Minimum biofilm eradication concentration (MBEC) of Q4-15a-1 is 4× MIC (16 μg/mL), indicating that Q4-15a-1 is effective against MDR ETEC biofilm. Besides, we established an MDR ETEC infection model with intestinal porcine epithelial cell-1 (IPEC-1). In this infection model, 32 μg/mL Q4-15a-1 can completely inhibit ETEC adhesion onto IPEC-1. Overall, these results suggested that Q4-15a-1 may be a promising antibacterial candidate for treatment of weaned piglets infected by MDR ETEC.

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“…In addition, recent findings described the presence of a pilus-like structure on the bacterial surface (Remuzgo-Mart ınez et al, 2014) and of cationic antimicrobial peptides (AMPs) (Tsai, Kuo, Lin, Lin, & Chen, 2015) as important mechanisms of Phdp pathogenicity.…”

Section: Introductionmentioning

confidence: 99%

“…The pathogenicity of Phdp is sustained by iron uptake mechanisms (Magarinos, Romalde, Lemos, Barja, & Toranzo, ), the presence of a polysaccharide capsular material (López‐Dóriga, Barnes, dos Santos, & Ellis, ) and the capability to produce an exotoxin effective against host macrophages and neutrophils (do Vale et al., ). In addition, recent findings described the presence of a pilus‐like structure on the bacterial surface (Remuzgo‐Martínez et al., ) and of cationic antimicrobial peptides (AMPs) (Tsai, Kuo, Lin, Lin, & Chen, ) as important mechanisms of Phdp pathogenicity . Rapid diagnosis of fish photobacteriosis is essential for a proper management and effective control of outbreaks in farmed fish (Andreoni & Magnani, ).…”

Section: Introductionmentioning

confidence: 99%

Development of a real‐time PCR assay for rapid detection and quantification of Photobacterium damselae subsp. piscicida in fish tissues

Carraro

Rovere

Ferraresso

et al. 2017

Journal of Fish Diseases

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The availability of a rapid and accurate method for the diagnosis of Photobacterium damselae subsp. piscicida (Phdp), able to discriminate its strictly correlated subsp. damselae (Phdd), formally known as Vibrio damsela, is essential for managing fish pasteurellosis outbreaks in farmed fish. A single-step, high-sensitivity real-time PCR assay for simultaneous detection and quantification of P. damselae was designed targeting partial of the sequence of the bamB gene and tested for specificity and sensitivity on laboratory-generated samples as well as on experimentally infected seabream tissue samples. With a limit of detection (LOD) of one copy in pure bacterial DNA, the sensitivity was higher than all methods previously reported. Validation in target and non-target bacterial species proved the assay was able to discriminate Phdd-Phdp subspecies from diverse hosts/geographical origins and between non-target species. In addition, two SNPs in the target amplicon region determine two distinctive qPCR dissociation curves distinguishing between Phdp-Phdd. This is the first time that a molecular method for P. damselae diagnosis combines detection, quantification and subspecies identification in one step. The assay holds the potential to improve the knowledge of infection dynamics and the development of better strategies to control an important fish disease.

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Photobacterium damselaesubsp.piscicidaresponds to antimicrobial peptides through phage-shock-protein A (PspA)-related extracytoplasmic stress response system

Cited by 7 publications

References 31 publications

A synthetic cationic antimicrobial peptide inhibits inflammatory response and the NLRP3 inflammasome by neutralizing LPS and ATP

A synthetic cationic antimicrobial peptide inhibits inflammatory response and the NLRP3 inflammasome by neutralizing LPS and ATP

Antibacterial and Antibiofilm Activities of Novel Antimicrobial Peptides against Multidrug-Resistant Enterotoxigenic Escherichia Coli

Development of a real‐time PCR assay for rapid detection and quantification of Photobacterium damselae subsp. piscicida in fish tissues

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