<i>Piriformospora indica</i>
            , a Cultivable Plant-Growth-Promoting Root Endophyte

Varma, Ajit; Verma, Savita; Sudha,; Sahay, Nirmal S.; Bütehorn, Britta; Franken, Philipp

doi:10.1128/aem.65.6.2741-2744.1999

Cited by 552 publications

(209 citation statements)

References 25 publications

Supporting

Mentioning

197

Contrasting

Unclassified

Order By: Relevance

“…We demonstrate here that species of the Sebacinales regularly undergo complex symbioses involving plants and bacteria of different genera. Sebacinoid fungi were formerly shown to possess a broad plant host spectrum (Varma et al, 1999;Weiss et al, 2004) and, so far investigated, exhibit beneficial biological activities to their hosts (Deshmukh et al, 2006). Our finding that the Sebacinoid symbiosis involves bacteria leads to a more complex picture and requires reconsideration of the role played by the fungus in its symbiotic interaction with the plant.…”

Section: Discussionmentioning

confidence: 90%

Detection and identification of bacteria intimately associated with fungi of the orderSebacinales

Sharma

Schmid

Rothballer

et al. 2008

Cellular Microbiology

151

137

View full text Add to dashboard Cite

Because of their beneficial impact on plants, the highly diverse mycorrhizal fungi grouped in the order Sebacinales lay claim to high ecological and agricultural significance. Here, we describe for the first time associations of Sebacinoid members with bacteria. Using quantitative PCR, denaturating gradient gel electrophoresis and fluorescence in situ hybridization, we detected an intimate association between Piriformospora indica and Rhizobium radiobacter, an alpha-Proteobacterium. The stability of the association, vertical transmission of the bacteria during asexual fungal reproduction and fungal plant colonization was monitored using R. radiobacter-specific primers. Treatment of mycelium or fungal protoplasts with antibiotics highly efficient against the free bacteria failed to cure the fungus. Barley seedlings dip-inoculated with R. radiobacter showed growth promotion and systemic resistance to the powdery mildew fungus Blumeria graminis comparable to P. indica inoculation. By screening additional isolates of the Sebacina vermifera complex, three species-specific associations with bacteria from the genera Paenibacillus, Acinetobacter and Rhodococcus were found. These findings suggest that Sebacinales species regularly undergo complex interactions involving host plants and bacteria reminiscent of other ectomycorrhizal and endomycorrhizal associations.

show abstract

Section: Discussionmentioning

confidence: 90%

Detection and identification of bacteria intimately associated with fungi of the orderSebacinales

Sharma

Schmid

Rothballer

et al. 2008

Cellular Microbiology

151

137

View full text Add to dashboard Cite

show abstract

“…By comparison, the effects of AMF on root-feeding insects are less well understood (Gange, 2001;Currie et al, 2011;Jung et al, 2012). Among the many nonpathogenic microbes that colonize roots without forming mycorrhizal structures, Piriformospora indica (Sebacinales) stands out because of its exceptionally broad host range and its positive effects on plant productivity via increases in plant tolerance to abiotic stresses and resistance to pathogens (Varma et al, 1999;Barazani et al, 2005;Waller et al, 2005;Qiang et al, 2012). P. indica manipulates particular branches of the signaling network of its host plants.…”

Section: Introductionmentioning

confidence: 99%

A fungal endophyte helps plants to tolerate root herbivory through changes in gibberellin and jasmonate signaling

et al. 2016

Self Cite

View full text Add to dashboard Cite

Summary Plant–microbe mutualisms can improve plant defense, but the impact of root endophytes on below‐ground herbivore interactions remains unknown. We investigated the effects of the root endophyte Piriformospora indica on interactions between rice (Oryza sativa) plants and its root herbivore rice water weevil (RWW; Lissorhoptrus oryzophilus), and how plant jasmonic acid (JA) and GA regulate this tripartite interaction.Glasshouse experiments with wild‐type rice and coi1‐18 and Eui1‐OX mutants combined with nutrient, jasmonate and gene expression analyses were used to test: whether RWW adult herbivory above ground influences subsequent damage caused by larval herbivory below ground; whether P. indica protects plants against RWW; and whether GA and JA signaling mediate these interactions.The endophyte induced plant tolerance to root herbivory. RWW adults and larvae acted synergistically via JA signaling to reduce root growth, while endophyte‐elicited GA biosynthesis suppressed the herbivore‐induced JA in roots and recovered plant growth.Our study shows for the first time the impact of a root endophyte on plant defense against below‐ground herbivores, adds to growing evidence that induced tolerance may be an important root defense, and implicates GA as a signal component of inducible plant tolerance against biotic stress.

show abstract

“…Over the last decade, considerable attention has been paid to the mutualistic fungus Piriformospora indica, which belongs to the order Sebacinales (Basidiomycota), and has been demonstrated to have a broad host range (Weiss et al, 2004). Piriformospora indica has been isolated from the rhizospheres of desert-adapted plants in India, and was found to be able to improve crop performance under controlled laboratory and field conditions by promoting growth and increasing stress tolerance (Verma et al, 1998;Varma et al, 1999;Waller et al, 2005;Serfling et al, 2007;Dolatabadi et al, 2011;Lee et al, 2011). The fungus can be easily cultured and shows no host specificity, a unique quality that suggests a wide variety of applications in biotechnology and plant production (Franken, 2012;Qiang et al, 2012a).…”

Section: Introductionmentioning

confidence: 99%

Sustained exposure to abscisic acid enhances the colonization potential of the mutualist fungus Piriformospora indica on Arabidopsis thaliana roots

et al. 2015

View full text Add to dashboard Cite

SummaryRoot colonization by the beneficial fungus Piriformospora indica is controlled by plant innate immunity, but factors that channel this interaction into a mutualistic relationship are not known. We have explored the impact of abscisic acid (ABA) and osmotic stress on the P. indica interaction with Arabidopsis thaliana.The activation of plant innate immunity in roots was determined by measuring the concentration of the phytoalexin camalexin and expression of transcription factors regulating the biosynthesis of tryptophan-related defence metabolites. Furthermore, the impact of the fungus on the content of ABA, salicylic acid, jasmonic acid (JA) and JA-related metabolites was examined.We demonstrated that treatment with exogenous ABA or the ABA analogue pyrabactin increased fungal colonization efficiency without impairment of plant fitness. Concomitantly, ABA-deficient mutants of A. thaliana (aba1-6 and aba2-1) were less colonized, while plants exposed to moderate stress were more colonized than corresponding controls. Sustained exposure to ABA attenuated expression of transcription factors MYB51, MYB122 and WRKY33 in roots upon P. indica challenge or chitin treatment, and prevented an increase in camalexin content.The results indicate that ABA can strengthen the interaction with P. indica as a consequence of its impact on plant innate immunity. Consequently, ABA will be relevant for the establishment and outcome of the symbiosis under stress conditions.

show abstract

Piriformospora indica , a Cultivable Plant-Growth-Promoting Root Endophyte

Cited by 552 publications

References 25 publications

Detection and identification of bacteria intimately associated with fungi of the orderSebacinales

Detection and identification of bacteria intimately associated with fungi of the orderSebacinales

A fungal endophyte helps plants to tolerate root herbivory through changes in gibberellin and jasmonate signaling

Sustained exposure to abscisic acid enhances the colonization potential of the mutualist fungus Piriformospora indica on Arabidopsis thaliana roots

Contact Info

Product

Resources

About