<i>Plantago asiatica mosaic virus</i> Found in Protected Crops of Lily Hybrids in Southern Italy

Parrella, G.; Greco, Béatrice; Pasqualini, Ana Paula de Azevedo; Nappo, A. G.

doi:10.1094/pdis-03-15-0281-pdn

Cited by 17 publications

(8 citation statements)

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“…Our study showed that up to five different viruses (LMoV, LSV, CMV, TVX, and PlAMV) can infect a single lily plant. To date, LMoV, LSV, and CMV are the most common viruses infecting lilies, while PlAMV resulting in serious damage to lily production is considered one of the rapidly spreading viruses in Lilium species [ 33 , 34 ]. Although cases of TVX and TRV infecting lilies have been reported [ 35 – 37 ], they have not been as well reported as the other four viruses.…”

Section: Discussionmentioning

confidence: 99%

RNA viromes of the oriental hybrid lily cultivar “Sorbonne”

Cho

2018

BMC Genomics

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BackgroundThe lily is a perennial flowering plant belonging to the genus Lilium in the family Liliaceae. Most cultivated lily plants are propagated by bulbs. Therefore, numerous lily bulbs are frequently infected by diverse viruses causing viral diseases. To date, no study has examined the viromes of plants of one type with identical genetic backgrounds collected from different geographical regions.ResultsHere, we examined different viromes of the lily cultivar “Sorbonne” using 172 gigabytes of transcriptome data composed of 23 libraries from four different projects for the cultivar “Sorbonne.” We identified 396 virus-associated contigs from all but one library. We identified six different viruses, including Plantago asiatica mosaic virus (PlAMV), Cucumber mosaic virus (CMV), Lily symptomless virus (LSV), Tulip virus X (TVX), Lily mottle virus (LMoV), and Tobacco rattle virus (TRV). Of them, PlAMV was the most common virus infecting the lily. Scale and flower samples possessed a high number of virus-associated reads. We assembled 32 nearly complete genomes for the six identified viruses possessing the polyadenylate tails. Genomes of all six viruses were highly conserved in the lily cultivar “Sorbonne” based on mutation analysis. We identified defective RNAs from LSV, TVX, and PlAMV localized in the triple gene block region. Phylogenetic analyses showed that virus genomes are highly correlated with geographical regions and host plants.ConclusionsWe conducted comprehensive virome analyses of a single lily cultivar, “Sorbonne,” using transcriptome data. Our results shed light on an array of lily virome-associated topics, including virus identification, the dominant virus, virus accumulation in different plant tissues, virus genome assembly, virus mutation, identification of defective RNAs, and phylogenetic relationships of identified viruses. Taken together, we provide very useful methods and valuable results that can be applied in other virome-associated studies.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5138-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

RNA viromes of the oriental hybrid lily cultivar “Sorbonne”

Cho

2018

BMC Genomics

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“…This sequence had 99% identity to PlAMV (KY807785). The result was confirmed using another RT‐PCR assay with primers corresponding to the PlAMV coat protein gene sequence (Parrella et al , 2015), and the same PCR master mix as used previously, to generate a 1 kb amplicon (MK005151). Sequence comparisons confirmed that the virus was PlAMV with 99% identity to Accession No.…”

Section: Figurementioning

confidence: 55%

Plantago asiatica mosaic virus detected in Lilium in the UK

Harju

Forde

Tozer

et al. 2018

New Disease Reports

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“…We hypothesize the virus was introduced with imported lily bulbs as has been indicated for other countries (Hammond et al 2015;Pájtli et al 2015;Parrella et al 2015;Vidal et al 2016;Xu et al 2017). Symptomless infection of lily was noted in Chile (Vidal et al 2016) and China (Xu et al 2017).…”

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confidence: 75%

“…Reports suggest that PlAMV is disseminated internationally by trading of infected bulbs (Hammond et al 2015;Pájtli et al 2015;Parrella et al 2015). Moreover, PlAMV is mechanically transmitted (by sap) and no arthropod vector is known.…”

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confidence: 99%