<i>Platanus</i> pollen allergen, Pla a 1: quantification in the atmosphere and influence on a sensitizing population

Fernández‐González, Delia; González-Parrado, Zulima; Vega-Maray, Ana María; Valencia-Barrera, Rosa María; Camazón-Izquierdo, B.; Nuntiis, Paola De; Mandrioli, Paolo

doi:10.1111/j.1365-2222.2010.03595.x

Cited by 57 publications

(38 citation statements)

References 31 publications

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“…Pla a1 is a non-glycosylated protein, while Pla a2 is a glycoprotein (Ibarrola et al 2004; Fernandez-Gonzalez et al 2010). Pla a1 represents ~60 % of the total IgE binding of Platanus pollen extract and can be used for specific diagnosis of Platanus ( orientalis and acerifolia ) pollen allergy (Asturias et al 2003; Pazouki et al 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Further, as the rapid growth of urban green areas continues, along with the number of plant species used, a corresponding increase in the rate of pollen allergies among patients has been reported (Riediker et al 2001). Several studies have shown that the increasing incidence of pollen allergies has become a global problem (Ishizaki et al 1978; Hwang et al 2005; Bosch-Cano et al 2011); for example, pollinosis has increasingly been found to have a profoundly negative effect on human health in Japan (Wang et al 2011), in Europe (Fernandez-Gonzalez et al 2010; Bosch-Cano et al 2011), and in north America (Ziska et al 2011). In China, the average incidence rate of hay fever is about 0.5–1 %; however, in particular areas, this figure can reach up to 5 %, such as in Wuhan, which is located in the center of China (Shi and Zhu 2009).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of protein expression of Platanus pollen following exposure to gaseous pollutants and vehicle exhaust particles

et al. 2014

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Being major ornamental street trees, species of Platanus are widely planted in the Shanghai urban area. A great deal of allergenic Platanus pollen is released from the trees and suspended in the atmosphere during its flowering season, ultimately causing allergic respiratory diseases. Few papers have focused on the distribution of this type of pollen and its expression of allergenic proteins. In order to investigate any differences in protein expression in Platanus pollen following exposure to gaseous and particulate pollutants, a special apparatus was designed. Exposure condition (such as temperature, humidity, and exposure time) of Platanus pollen and gaseous pollutants can be simulated using of this apparatus. Fresh Platanus orientalis pollen, pollutant gases (NO2, SO2, NH3), and typical urban ambient particles (vehicle exhaust particles, VEPs) were mixed in this device to examine possible changes that might occur in ambient airborne urban pollen following exposure to such pollutants. Our results showed that the fresh P. orientalis pollen became swollen, and new kinds of particles could be found on the surface of the pollen grains after exposure to the pollutants. The results of SDS-PAGE showed that five protein bands with molecular weights of 17–19, 34, 61, 82, and 144 kDa, respectively, were detected and gray scale of these brands increased after the pollen exposure to gaseous pollutants. The two-dimensional gel electrophoresis analysis demonstrated that a Platanus pollen allergenic protein (Pla a1, with a molecular weight of 18 kDa) increased in abundance following exposure to pollutant gases and VEPs, implying that air pollutants may exacerbate the allergenicity of pollen.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of protein expression of Platanus pollen following exposure to gaseous pollutants and vehicle exhaust particles

et al. 2014

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“…As a result, a variety of immunoassays that utilize different assay formats have been developed and enabled the quantification of pollen allergens in air samples (Holmquist and Vesterberg, 1999; Marth et al, 2004; Fernandez-Gonzalez et al, 2010). Clinically, the quantification of allergen may be more relevant than direct pollen counts especially as pollen fragments may be quantified (Taylor et al, 2007).…”

Section: Alternative Methodsmentioning

confidence: 99%

Immunologic, spectrophotometric and nucleic acid based methods for the detection and quantification of airborne pollen

Rittenour

Hamilton

Beezhold

et al. 2012

Journal of Immunological Methods

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Microscopic identification of pollen morphological phenotypes has been the traditional method used to identify and quantify pollen collected by air monitoring stations worldwide. Although this method has enabled a semi-standardized approach for the assessment of pollen exposure, limitations including labor intensiveness, required expertise, examiner bias, and the inability to differentiate species, genera, and in some cases families have limited data derived from the these stations. Recent advances in chemical, biochemical and molecular detection methods have provided standardized alternatives to this microscopic approach. In this review, we examine the applicability of alternative methodologies, in particular nucleic acid based assays involving the quantitative polymerase chain reaction, for the standardized detection of airborne pollen.

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“…Although it is not the first publication on the subject it might be hoped that the study of Fernandez-Gonzalez et al [175], leads to widespread introduction of allergen measurement into aerobiology investigations and monitoring. Many allergic and non-allergic people are only too familiar with the pollination behaviour of the plain trees that grace the streets of many European and North American cities.…”

Section: Aeroallergensmentioning

confidence: 99%

Developments in the field of allergy in 2010 through the eyes of Clinical and Experimental Allergy

Katelaris

Linneberg

Magnan

et al. 2011

Clin Experimental Allergy

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In 2010 over 200 articles were published in Clinical and Experimental Allergy including editorials, reviews, opinion articles, letters, book reviews and of course at the heart of the journal, papers containing original data which have moved the field of allergy forward on a number of fronts. For the third year running the editors felt it would be of value to summarize the key messages contained in these papers as a snapshot of where the cutting edge of research into allergic disease is leading. We have broadly followed the sections of the journal, although this year the mechanistic articles are grouped together and the studies involving experimental models of disease are discussed throughout the paper. In the field of asthma and rhinitis phenotypes and biomarkers continue to a major pre-occupation of our authors. There is continued interest in mechanisms of inflammation and disordered lung function with the mouse model of asthma continuing to offer new insights. There is also a steady flow of papers investigating new therapies, including those derived from plants and herbs, although many are mechanistic with too few high quality clinical trials. The mechanisms involved in allergic disease are well covered with many strong papers using clinical material to ask relevant questions. Pro-pre and snybiotics continue to be of major interest to our authors and this remains a controversial and complicated field. The discipline of epidemiology has retained its interest in risk factors for the development of allergic disease with a view to refining and debating the reasons for the allergy epidemic. There is continued interest in the relationship between helminthic disease and allergy with a new twist in 2010 involving studies using infection with helminths as a potential treatment. The genetics of allergic disease continues to be very productive, although the field has moved on from only investigating single nucleotide polymorphisms of candidate genes to Genome Wide Association Studies and an increasing and welcome emphasis on gene-environment interactions. In the field of clinical allergy there is steady flow of papers describing patterns of drug allergy with renewed interest in reactions to contrast media, but food allergy is the major area of interest in this section of the journal. Lastly in the field of allergens there is a growing interest in the role of component resolved diagnosis in improving the diagnosis and management of allergic disease. Another excellent year, full of fascinating and high quality work, which the journal has been proud to bring to the allergy community.

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Platanus pollen allergen, Pla a 1: quantification in the atmosphere and influence on a sensitizing population

Cited by 57 publications

References 31 publications

Characterization of protein expression of Platanus pollen following exposure to gaseous pollutants and vehicle exhaust particles

Characterization of protein expression of Platanus pollen following exposure to gaseous pollutants and vehicle exhaust particles

Immunologic, spectrophotometric and nucleic acid based methods for the detection and quantification of airborne pollen

Developments in the field of allergy in 2010 through the eyes of Clinical and Experimental Allergy

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