<i>Pleurotus ostreatus</i> bHLH transcription factors regulate plant growth and development when expressed in Arabidopsis

Chen, Siyu; Yang, Wenting; Jia, Qiming; Wang, Wei; Zhang, Na; Wang, Xutong; Wang, Shucai

doi:10.1080/17429145.2017.1400124

Cited by 6 publications

(3 citation statements)

References 42 publications

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“…Also, the AtNFL promotes flowering under short-day conditions in Arabidopsis [14]. The PobHLH5 and PobHLH8 of P. ostreatus infestation in Arabidopsis verified that bHLH genes play an important role in growth and development [15]. In addition, bHLHs are also involved in plant abiotic stresses, signal transmission and secondary metabolism, etc.…”

Section: Introductionmentioning

confidence: 85%

Genome-Wide Analysis of bHLH Gene Family in Loropetalum chinense var. Rubrum: Identification, Classification, Evolution, and Expression Pattern Diversity on Cultivations

Li¹,

Yang²,

Mo³

et al. 2023

Preprint

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The basic helix-loop-helix (bHLH) transcription factor family is the second-largest transcription factor in plants. Members of this family are involved in the process of growth and development, secondary metabolic biosynthesis, signal transduction, and plant resistance. Loropetalum chinense var. rubrum is a critical woody plant with higher ornamental and economic values, which used as ornamental architecture and traditional Chinese herbal medicine plant. However, the bHLH transcription factor in the L. chinense var. rubrum (Loropetalum chinense var. Rubrum) have yet to be systematically demonstrated, and their role in anthocyanin biosynthesis remains secret. Here, we identified 165 potential LcbHLHs genes by two methods, and they were unequally distributed on chromosomes 1 to 12 of the L. chinense genome. Based on a phylogenetic comparison with proteins from Arabidopsis, these bHLH proteins were categorized into 21 subfamilies. Most LcbHLHs in a given subfamily had similar gene structures and conserved motifs. The gene ontology annotation and cis-elements predicted that the LcbHLHs had many molecular functions and were involved in plant growth processes, including flavonoid and anthocyanin biosynthetic processes, etc. Transcriptomic analysis revealed different expression patterns among different tissues and cultivars in L. chinense. Many LcbHLHs were expressed in leaves, and only a few genes were highly expressed in flowers. Six LcbHLH genes differentially expressed between species and periods in color variation, which may play a key role in anthocyanin synthesis. These further functional predictions of LcbHLHs were established by quantitative real-time PCR analysis and protein-protein interaction network. This study identified the six potential bHLH genes related to anthocyanin in L. chinense and created a solid framework for future research on the function and evolution of bHLHs.

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Section: Introductionmentioning

confidence: 85%

Genome-Wide Analysis of bHLH Gene Family in Loropetalum chinense var. Rubrum: Identification, Classification, Evolution, and Expression Pattern Diversity on Cultivations

Li¹,

Yang²,

Mo³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…Moreover, AtNFL promotes flowering under short-day conditions in Arabidopsis [14]. The PobHLH5 and PobHLH8 of P. ostreatus infestation in Arabidopsis verified that bHLH genes play an important role in growth and development [15]. In addition, bHLHs are also involved in plants' abiotic stress responses, signal transmission, secondary metabolism, etc.…”

Section: Introductionmentioning

confidence: 85%

Genome-Wide Analysis of the bHLH Gene Family in Loropetalum chinense var. rubrum: Identification, Classification, Evolution, and Diversity of Expression Patterns under Cultivation

Liu,

Lin,

Liu

et al. 2023

Plants

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The basic helix–loop–helix (bHLH) transcription factor family is the second-largest transcription factor family in plants. Members of this family are involved in the processes of growth and development, secondary metabolic biosynthesis, signal transduction, and plant resistance. Loropetalum chinense var. rubrum is a critical woody plant with higher ornamental and economic values, which has been used as ornamental architecture and traditional Chinese herbal medicine plants. However, the bHLH transcription factors in Loropetalum chinense var. rubrum (L. chinense var. rubrum) have not yet been systematically demonstrated, and their role in the biosynthesis of anthocyanin is still unclear. Here, we identified 165 potential LcbHLHs genes by using two methods, and they were unequally distributed on chromosomes 1 to 12 of the genome of L. chinense var. rubrum. Based on an evolutionary comparison with proteins from Arabidopsis and Oryza sativa, these bHLH proteins were categorized into 21 subfamilies. Most LcbHLHs in a particular subfamily had similar gene structures and conserved motifs. The Gene Ontology annotation and Cis-elements predicted that LcbHLHs had many molecular functions and were involved in processes of plant growth, including the biosynthesis of flavonoids and anthocyanins. Transcriptomic analysis revealed different expression patterns among different tissues and cultivars of L. chinense var. rubrum. Many LcbHLHs were expressed in the leaves, and only a few genes were highly expressed in the flowers. Six LcbHLHs candidate genes were identified by bioinformatics analysis and expression analysis. Further Real-time quantitative PCR analysis and protein interaction network analysis showed that LcbHLH156, which is one of the candidate proteins belonging to the IIIf subfamily, could interact with proteins related to anthocyanin synthesis. Therefore, LcbHLH156 was transiently expressed in L. chinense var. rubrum to verify its function in regulating anthocyanin synthesis. Compared with the control group, red pigment accumulation appeared at the wound after injection, and the total anthocyanin content increased at the wound of leaves. These results lay a foundation for the research of the regulation mechanism of leaf colors in L. chinense var. rubrum and also provide a basis for the function of the LcbHLH family.

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“…Its core conserved domain contains approximately 60 amino acid residues [6]. The bHLH has been shown to be involved in numerous biological processes, including stress resistance [7][8][9], growth and development [10,11], signal transduction [12,13] and synthesis [14][15][16]. The first bHLH transcription factor was discovered in maize, where its function is involved in the synthesis of anthocyanins [17].…”

Section: Introductionmentioning

confidence: 99%

Anthocyanin Synthesis and the Expression Patterns of bHLH Transcription Factor Family during Development of the Chinese Jujube Fruit (Ziziphus jujuba Mill.)

Shi

et al. 2019

Forests

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The basic helix–loop–helix (bHLH) family is an important transcription factor for eukaryotes and is involved in a wide range of biological activities. Among these, bHLH can interaction with WD repeat (WD40 or WDR) and V-myb avian myeloblastosis viral oncogene homolog (MYB) form a ternary complex to promote the efficient synthesis of anthocyanins. In this study, a total of 138 jujube bHLH (ZjbHLH) family members were screened from the transcriptome of the two jujube cultivars, ‘Junzao’ (JZ) and ‘Tailihong’ (TLH). Of these, 95 ZjbHLH genes were mapped to 12 chromosomes. A phylogenetic tree was constructed using 27 arabidopsis bHLH (AtbHLH) protein sequences of Arabidopsis thaliana (L.) Heynh. and 138 ZjbHLH protein sequences of jujube. The results show that the ZjbHLH family of jujube can be divided into 12 subfamilies. The three candidate genes, ZjGL3a, ZjGL3b and ZjTT8, related to anthocyanin synthesis, were classified into subgroup III. Meanwhile, ZjGL3a, ZjGL3b and ZjTT8 have high homology with the bHLH transcription factors involved in anthocyanin synthesis in other plants. In addition, it was found that the jujube ZjbHLH transcript family showed changing patterns of expression during fruit development. The relative expression levels of ZjGL3a, ZjGL3 and ZjTT8 were consistent with the changes of the anthocyanin contents in the two jujube cultivars examined. To better understand the anthocyanin synthesis pathway involved in ZjbHLH, a regulatory pathway model for anthocyanin synthesis was constructed. This model involves the processes of anthocyanin signal transduction, synthesis and transport.

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Pleurotus ostreatus bHLH transcription factors regulate plant growth and development when expressed in Arabidopsis

Cited by 6 publications

References 42 publications

Genome-Wide Analysis of bHLH Gene Family in Loropetalum chinense var. Rubrum: Identification, Classification, Evolution, and Expression Pattern Diversity on Cultivations

Genome-Wide Analysis of bHLH Gene Family in Loropetalum chinense var. Rubrum: Identification, Classification, Evolution, and Expression Pattern Diversity on Cultivations

Genome-Wide Analysis of the bHLH Gene Family in Loropetalum chinense var. rubrum: Identification, Classification, Evolution, and Diversity of Expression Patterns under Cultivation

Anthocyanin Synthesis and the Expression Patterns of bHLH Transcription Factor Family during Development of the Chinese Jujube Fruit (Ziziphus jujuba Mill.)

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