<i>Populus</i> D-type cyclin gene <i>PsnCYCD1;1</i> accelerates cell division and participates in secondary growth of vascular bundles

Zheng, Tangchun; Dai, Liyun; Li, Shuang; Liu, Yi; Zhao, Zhongnan; Yang, Chuanping; Qu, Guanzheng

doi:10.1093/jxb/erad140

Cited by 3 publications

(1 citation statement)

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“…In plants, D-type cyclins are crucial for growth and development and regulate the cell division process through CDK-CYCD. Overexpression of the Populus D-type cyclin PsnCYCD1;1 gene in Arabidopsis can promote cell division and lead to small cell generation [45], and PsnCYCD1;1 overexpression in plants could accelerate cell division, causing the generation of small cells and severe morphological changes in the vascular bundles [46]. Overexpression of PtoCYCD3;3 increases the thickness of secondary xylem and phloem by increasing cambium cell activity, and it may interact with 12 PtoCDK proteins to regulate cell cycle programming [47].…”

Section: Cyclins Play a Role In Cell Divisionmentioning

confidence: 99%

Phenotypic Analysis and Molecular Characterization of Enlarged Cell Size Mutant in Nannochloropsis oceanica

Xu,

Lin,

Wang

et al. 2023

IJMS

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The cell cycle is the fundamental cellular process of eukaryotes. Although cell-cycle-related genes have been identified in microalgae, their cell cycle progression differs from species to species. Cell enlargement in microalgae is an essential biological trait. At the same time, there are various causes of cell enlargement, such as environmental factors, especially gene mutations. In this study, we first determined the phenotypic and biochemical characteristics of a previously obtained enlarged-cell-size mutant of Nannochloropsis oceanica, which was designated ECS. Whole-genome sequencing analysis of the insertion sites of ECS indicated that the insertion fragment is integrated inside the 5′-UTR of U/P-type cyclin CYCU;1 and significantly decreases the gene expression of this cyclin. In addition, the transcriptome showed that CYCU;1 is a highly expressed cyclin. Furthermore, cell cycle analysis and RT-qPCR of cell-cycle-related genes showed that ECS maintains a high proportion of 4C cells and a low proportion of 1C cells, and the expression level of CYCU;1 in wild-type (WT) cells is significantly increased at the end of the light phase and the beginning of the dark phase. This means that CYCU;1 is involved in cell division in the dark phase. Our results explain the reason for the larger ECS size. Mutation of CYCU;1 leads to the failure of ECS to fully complete cell division in the dark phase, resulting in an enlargement of the cell size and a decrease in cell density, which is helpful to understand the function of CYCU;1 in the Nannochloropsis cell cycle.

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Section: Cyclins Play a Role In Cell Divisionmentioning

confidence: 99%