<i>Prunus domestica</i>
            plum.

Petri, César; Ruiz, David; Faize, Mohamed; Burgos, L.; Alburquerque, Nuria

doi:10.1079/9781780648279.0512

Cited by 2 publications

(1 citation statement)

References 89 publications

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“…Sharka disease caused by the plum pox virus (PPV) is the most important disease of stone fruit, and the establishment of new cultivars resistant to sharka is one of the most focused topics in European plum breeding programs [53]. Among the different transgenic strategies used to achieve PPV resistance, successful results have been obtained via applications of RNA silencing techniques [54].…”

Section: Genetic Transformation Technology In Fruit Treesmentioning

confidence: 99%

CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding

Martín-Valmaseda,

Devin,

Ortuño-Hernández

et al. 2023

IJMS

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CRISPR (short for “Clustered Regularly Interspaced Short Palindromic Repeats”) is a technology that research scientists use to selectively modify the DNA of living organisms. CRISPR was adapted for use in the laboratory from the naturally occurring genome-editing systems found in bacteria. In this work, we reviewed the methods used to introduce CRISPR/Cas-mediated genome editing into fruit species, as well as the impacts of the application of this technology to activate and knock out target genes in different fruit tree species, including on tree development, yield, fruit quality, and tolerance to biotic and abiotic stresses. The application of this gene-editing technology could allow the development of new generations of fruit crops with improved traits by targeting different genetic segments or even could facilitate the introduction of traits into elite cultivars without changing other traits. However, currently, the scarcity of efficient regeneration and transformation protocols in some species, the fact that many of those procedures are genotype-dependent, and the convenience of segregating the transgenic parts of the CRISPR system represent the main handicaps limiting the potential of genetic editing techniques for fruit trees. Finally, the latest news on the legislation and regulations about the use of plants modified using CRISPR/Cas systems has been also discussed.

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Section: Genetic Transformation Technology In Fruit Treesmentioning

confidence: 99%

CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding

Martín-Valmaseda,

Devin,

Ortuño-Hernández

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

CRISPR as Genome Editing Technique in Fruit Trees Breeding

Martín-Valmaseda,

Devin,

Ortuño-Hernández

et al. 2023

Preprint

View full text Add to dashboard Cite

The methods used to introduce CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas-mediated genome editing into fruit species, as well as the impacts of the application of this technology to activate and knock out of target genes in different fruit trees species including tree development, yield, fruit quality, and tolerance to biotic and abiotic stresses have been firstly described in this review. The application of this gene editing technology could allow the development of new generations of fruit crops with improved traits by targeting different genetic segments or even could facilitate the introduction of traits in elite cultivars without changing other traits. However, at this moment, the scarcity of efficient regeneration and transformation protocols in some species, the fact that many of those procedures are the genotype-dependent or the convenience of segregating the transgenic parts of the CRISPR system represent the main handicaps limiting the potential of genetic editing techniques for fruit trees. Finally, latest news on the legislation and regulations about the use of plants modified through CRISPR/Cas systems has been also discussed.

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Prunus domestica plum.

Abstract: This chapter discusses the botany and history, breeding and genetics, molecular genetics, gene cloning, genomics, disease resistance, nematode resistance, micropropagation, micrografting, somatic cell genetics, genetic manipulation, genetic transformation and cryopreservation of plum.

Cited by 2 publications

References 89 publications

CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding

CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding

CRISPR as Genome Editing Technique in Fruit Trees Breeding

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