<i>Pseudomonas aeruginosa</i>in the home environment of newly infected cystic fibrosis patients

Schelstraete, Petra; Daele, S. Van; Boeck, K. De; Proesmans, Marijke; Lebecque, Patrick; Leclercq-Foucart, J; Malfroot, Anne; Vaneechoutte, Mario; Baets, Frans De

doi:10.1183/09031936.00088907

Cited by 54 publications

(55 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the characteristics of Pseudomonas aeruginosa is high resistance to the types of antibiotics (Poole, 2004;Estahbanati, Kashani, & Ghanaatpisheh, 2002;Ebrahimzadeh Shiraz, Rezaei Yazdi, & Alijanianzadeh, 2017). Schelstraete et al showed that patients with cystic fibrosis had received Pseudomonas aeruginosa out of the home environment (such as the dental environment) (Schelstraete et al, 2007). Therefore, the transmission of this pathogen to patients with weakened immune system in dental centers is a great concern.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Disinfection Quality of Dental Faculty Units of Ahvaz Jundishapur University of Medical Sciences, Southwest of Iran in 2017

Bagheri¹,

Shooriabi²,

Amin³

et al. 2018

JMBR

View full text Add to dashboard Cite

Introduction: Performing dental practices in the oral environment leads to the transmission of microorganisms in saliva and blood to working surfaces and dental devices and their infection. Preventing transmission of infection through these devices is an important task of a dentist. Hence, this research was conducted to evaluate the disinfection quality of the dental faculty units of Ahvaz Jundishapur University of Medical Sciences (AJUMS) in Iran. Materials and Methods:In order to evaluate the quality of disinfection of the units, sampling was performed from all glasses spittoon surfaces of 90 units of the clinical unit of the AJUMS dental faculty before and after disinfection by personnel. Then, the bacteria were cultured in a medium and examined. Results:The mean (and standard deviation) of the total infection of units of the dental faculty was 46534.4 (583380.4) colonies per 1 ml before disinfection and 40265.6 (52131.1) colonies per 1 ml after disinfection, reflecting significant decrease in number of bacterial colonies after disinfection (P <0.001). In addition, a significant decrease was seen in the number of bacterial colonies in the restoration, pediatric, orthodontic and diagnosis units (p <0.05), but the difference before and after disinfection was not significant in the prosthetic, endodontic, surgical and periodontal units. In addition, the most common types of microorganisms in the whole units of the dental faculty were pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus, respectively, and after disinfection, the most common types of microorganisms were Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus, respectively. Conclusion:In general, this study showed that the disinfection method of units in dental faculty can not reduce the severity of infection of the units. Given what was stated, it is recommended that the method and the substances used to disinfect the unit to be changed.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of Disinfection Quality of Dental Faculty Units of Ahvaz Jundishapur University of Medical Sciences, Southwest of Iran in 2017

Bagheri¹,

Shooriabi²,

Amin³

et al. 2018

JMBR

View full text Add to dashboard Cite

show abstract

“…In domestic environments, A. xylosoxidans was detected in hand-washing sinks and shower drains but not in toilet bowls. The colonization of sinks and shower drains by P. aeruginosa has already been described in home environment studies (43,44). These materials are in contact with soap as well as with chemical products used for cleaning, many of them containing alkyldimethylbenzylammonium chloride, a quaternary ammonium compound.…”

Section: Discussionmentioning

confidence: 99%

Detection of Achromobacter xylosoxidans in Hospital, Domestic, and Outdoor Environmental Samples and Comparison with Human Clinical Isolates

Amoureux

Bador

Fardeheb

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

Achromobacter xylosoxidans is an aerobic nonfermentative Gram-negative rod considered an important emerging pathogen among cystic fibrosis (CF) patients worldwide and among immunocompromised patients. This increased prevalence remains unexplained, and to date no environmental reservoir has been identified. The aim of this study was to identify potential reservoirs of A. xylosoxidans in hospital, domestic, and outdoor environments and to compare the isolates with clinical ones. From 2011 to 2012, 339 samples were collected in Dijon's university hospital, in healthy volunteers' homes in the Dijon area, and in the outdoor environment in Burgundy (soil, water, mud, and plants). We designed a protocol to detect A. xylosoxidans in environmental samples based on a selective medium: MCXVAA (MacConkey agar supplemented with xylose, vancomycin, aztreonam, and amphotericin B). Susceptibility testing, genotypic analysis by pulsed-field gel electrophoresis, and bla OXA-114 sequencing were performed on the isolates. A total of 50 strains of A. xylosoxidans were detected in hospital (33 isolates), domestic (9 isolates), and outdoor (8 isolates) samples, mainly in hand washing sinks, showers, and water. Most of them were resistant to ciprofloxacin (49 strains). Genotypic analysis and bla OXA-114 sequencing revealed a wide diversity among the isolates, with 35 pulsotypes and 18 variants of oxacillinases. Interestingly, 10 isolates from hospital environment were clonally related to clinical isolates previously recovered from hospitalized patients, and one domestic isolate was identical to one recovered from a CF patient. These results indicate that A. xylosoxidans is commonly distributed in various environments and therefore that CF patients or immunocompromised patients are surrounded by these reservoirs.

show abstract

“…304 Similarly, in the homes of people with CF, P. aeruginosa was detected most often from shower drains and bathroom drainpipes. 305,306 While P. aeruginosa was recovered from 34% of the homes of newly infected people with CF (6% of samples), only 9 (18%) of 50 paired environmental and patient isolates were the same strain. 306 It remains unclear whether these P. aeruginosa strains were transmitted from the individuals with CF to the home environment or vice versa, but it suggests that cleaning and disinfection efforts in the homes of people with CF should focus on bathroom drains.…”

Section: Iiid1 Nonhealthcare Sources: Soil Organic Matter and Watermentioning

confidence: 99%

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Saiman

Siegel

LiPuma

et al. 2014

Infect. Control Hosp. Epidemiol.

352

382

View full text Add to dashboard Cite

The 2013 Infection Prevention and Control (IP&C) Guideline for Cystic Fibrosis (CF) was commissioned by the CF Foundation as an update of the 2003 Infection Control Guideline for CF. During the past decade, new knowledge and new challenges provided the following rationale to develop updated IP&C strategies for this unique population:1. The need to integrate relevant recommendations from evidence-based guidelines published since 2003 into IP&C practices for CF. These included guidelines from the Centers for Disease Control and Prevention (CDC)/Healthcare Infection Control Practices Advisory Committee (HICPAC), the World Health Organization (WHO), and key professional societies, including the Infectious Diseases Society of America (IDSA) and the Society for Healthcare Epidemiology of America (SHEA). During the past decade, new evidence has led to a renewed emphasis on source containment of potential pathogens and the role played by the contaminated healthcare environment in the transmission of infectious agents. Furthermore, an increased understanding of the importance of the application of implementation science, monitoring adherence, and feedback principles has been shown to increase the effectiveness of IP&C guideline recommendations.2. Experience with emerging pathogens in the non-CF population has expanded our understanding of droplet transmission of respiratory pathogens and can inform IP&C strategies for CF. These pathogens include severe acute respiratory syndrome coronavirus and the 2009 influenza A H1N1. Lessons learned about preventing transmission of methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant gram-negative pathogens in non-CF patient populations also can inform IP&C strategies for CF.

show abstract

Pseudomonas aeruginosain the home environment of newly infected cystic fibrosis patients

Cited by 54 publications

References 35 publications

Evaluation of Disinfection Quality of Dental Faculty Units of Ahvaz Jundishapur University of Medical Sciences, Southwest of Iran in 2017

Evaluation of Disinfection Quality of Dental Faculty Units of Ahvaz Jundishapur University of Medical Sciences, Southwest of Iran in 2017

Detection of Achromobacter xylosoxidans in Hospital, Domestic, and Outdoor Environmental Samples and Comparison with Human Clinical Isolates

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Contact Info

Product

Resources

About