<i>PSEUDOMONAS AUREOFACIENS</i>
            KLUYVER AND PHENAZINE α-CARBOXYLIC ACID, ITS CHARACTERISTIC PIGMENT

Conway, H. F.; Haynes, William C.; Jackson, Richard W.; Locke, Joan M.; Pridham, T. G.; Sohns, V. E.; Stodola, Frank H.

doi:10.1128/jb.72.3.412-417.1956

Cited by 54 publications

(24 citation statements)

References 5 publications

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“…There is considerable evidence that pseudomonads produce a number of antimicrobial compounds (Howell & Stipanovie, 1980;Salcher & Lingens, 1980). In 1956 phenazine-1-carboxylic aeid, produced by a pseudomonad, was tested against a number of plant pathogens and shown to be toxic at concentrations of 75 A^g/ml (Haynes et al, 1956). Our results demonstrate that a eompound absorbing at 365 nm was produced by fluorescent pseudomonads under axenie conditions in sand supporting wheat roots.…”

Section: Discussionmentioning

confidence: 65%

Mechanisms of inhibition of Gaeumannomyces graminis var. tritici by fluorescent pseudomonads

Brisbane

Rovira

1988

Plant Pathology

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The take-all fungus Gaei^niannomyces gratniuis var. tritici reduced the weight of wheat plants grown in tubes containing sterilized sand and plant nutrient solution. Fiuorescent pseudomonads, when added to the tubes, inhibited the take-ali fungus and increased piant weight. Iron (as FeNaEDTA) had no effect on the inhibition ofthe fungus by the pseudomonads. Some ofthe pseudomonads produced a compound in the wheat rhizosphere with a UV absorption peak at 365 nm, but the inhibition of G. graminis by pseudomonads was not proportionai to the UV absorbance of rhizosphere extracts. A yeiiow crystaiiine compound, absorbing at 365 nm, was extracted from broth cuitures and shown to be toxic to G. graminis under acid conditions. This compound is considered to be phenazine-i-carboxylic acid.

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Section: Discussionmentioning

confidence: 65%

Mechanisms of inhibition of Gaeumannomyces graminis var. tritici by fluorescent pseudomonads

Brisbane

Rovira

1988

Plant Pathology

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“…Phenazine‐1‐carboxylic acid was first isolated from a strain of Pseudomonas aureofaciens 62, 63. Isono et al 64 also demonstrated the production of phenazine‐1‐carboxylic acid, named tubermycin B, from cultures of Streptomyces misakiensis Nakamura.…”

Section: Discussionmentioning

confidence: 99%

Isolation and in vitro and in vivo activity against Phytophthora capsici and Colletotrichum orbiculare of phenazine‐1‐carboxylic acid from Pseudomonas aeruginosa strain GC‐B26

Lee

Moon

Hwang

2003

Pest Management Science

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The bacterial strain GC-B26, which showed strong antifungal and anti-oomycete activity against some plant pathogens, was isolated from a grassland soil in Korea. Based on morphological, physiological and biochemical characteristics, GC-B26 was identical to Pseudomonas aeruginosa (Schroeter) Migula. The antibiotic G26A, active against Phytophthora capsici Leonian and Colletotrichum orbiculare (Berk & Mont) van Arx, was isolated from the culture filtrates of Ps aeruginosa strain GC-B26 using various chromatographic procedures. The EI mass and UV spectral results indicated that G26A is an analogue of phenazines, having molecular formula C13H8N2O2 (M+, m/z 224.0664). On the basis of NMR spectral data, G26A was confirmed as phenazine-1-carboxylic acid. C orbiculare, P capsici and Pythium ultimum Trow were most sensitive to G26A, with MIC values of approximately 5 microg ml(-1). However, no antimicrobial activity was found against yeasts and bacteria, even at a concentration of over 100 microg ml(-1). Treatment with the antibiotic gave highly significant protective activity against the development of Phytophthora disease on pepper and anthracnose on cucumber plants. The disease control efficacy was only slightly less than that of the commercial fungicides metalaxyl and chlorothalonil.

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“…However, these studies did not address the mechanism by which phenazine antibiotics enhance the survival of the producing strain. The phenazine antibiotics produced by strains 2-79 and 30-84 have excellent activity against a variety of microorganisms, including a number of fungi and bacteria that are commonly isolated from the rhizosphere of wheat (17,18). Therefore, studies were conducted in soil pasteurized with moist heat to test the hypothesis that the enhanced survival of phenazine-producing strains was due to an improved capability to compete with indigenous microorganisms.…”

Section: Discussionmentioning

confidence: 99%

“…known. PCA has broad-spectrum activity against bacteria and fungi (17,18) and therefore may be a factor in the ability of producer pseudomonads to compete with other soil or rhizosphere microorganisms. It has been suggested that antibiotic production may aid in the initial colonization of a substrate by soil microorganisms (3).…”

mentioning

confidence: 99%

Contribution of phenazine antibiotic biosynthesis to the ecological competence of fluorescent pseudomonads in soil habitats

Mazzola

Cook

Thomashow

et al. 1992

Appl Environ Microbiol

345

147

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Phenazine antibiotics produced by Pseudomonas fluorescens 2-79 and Pseudomonas aureofaciens 30-84, previously shown to be the principal factors enabling these bacteria to suppress take-all of wheat caused by Gaeumannomyces graminis var. tritici, also contribute to the ecological competence of these strains in soil and in the rhizosphere of wheat. Strains 2-79 and 30-84, their TnS mutants defective in phenazine production (Phz-), or the mutant strains genetically restored for phenazine production (Phz+) were introduced into Thatuna silt loam (TSL) or TSL amended with G. graminis var. tritici. Soils were planted with three or five successive 20-day plant-harvest cycles of wheat. Population sizes of Phzderivatives declined more rapidly than did population sizes of the corresponding parental or restored Phz+ strains. Antibiotic biosynthesis was

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PSEUDOMONAS AUREOFACIENS KLUYVER AND PHENAZINE α-CARBOXYLIC ACID, ITS CHARACTERISTIC PIGMENT

Cited by 54 publications

References 5 publications

Mechanisms of inhibition of Gaeumannomyces graminis var. tritici by fluorescent pseudomonads

Mechanisms of inhibition of Gaeumannomyces graminis var. tritici by fluorescent pseudomonads

Isolation and in vitro and in vivo activity against Phytophthora capsici and Colletotrichum orbiculare of phenazine‐1‐carboxylic acid from Pseudomonas aeruginosa strain GC‐B26

Contribution of phenazine antibiotic biosynthesis to the ecological competence of fluorescent pseudomonads in soil habitats

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