<i>Pseudomonas pseudomallei</i>isolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping

Currie, Bart J.; Smith‐Vaughan, Heidi; Golledge, C.L.; Buller, Nicky; Sriprakash, Kadaba S.; Kemp, David J.

doi:10.1017/s0950268800051736

Cited by 69 publications

(58 citation statements)

References 16 publications

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“…10 Previously we used ribotyping to show clonality in a cluster of isolates from farm animals, a farmer, and soil in the temperate southwest of Australia. 11 That cluster was presumed to have been introduced to the non-endemic region by importation of an infected animal, but the current case series occurred within the hyperendemic region of tropical northern Australia, where there is large genetic diversity amongst human, animal, and environmental B. pseudomallei isolates, as shown by the soil isolates and previous studies. 6 The mode of transmission of B. pseudomallei in this outbreak remains speculative, but appears likely to have originated from persisting contamination of the community water supply, possibly due to biofilm protection and propagation of bacterial colonies within the water supply system.…”

Section: Discussionmentioning

confidence: 83%

A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates.

Currie

Mayo²,

Anstey³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

129

115

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Abstract. Nine cases of melioidosis with four deaths occurred over a 28-month period in members of a small remote Aboriginal community in the top end of the Northern Territory of Australia. Typing by pulsed-field gel electrophoresis showed isolates of Burkholderia pseudomallei from six of the cases to be clonal and also identical to an isolate from the community water supply, but not to soil isolates. The clonality of the isolates found in this cluster contrasts with the marked genetic diversity of human and environmental isolates found in this region which is hyperendemic for B. pseudomallei. It is possible that the clonal bacteria persisted and were propagated in biofilm in the water supply system. While the exact mode of transmission to humans and the reasons for cessation of the outbreak remain uncertain, contamination of the unchlorinated community water supply is a likely explanation.

show abstract

Section: Discussionmentioning

confidence: 83%

A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates.

Currie

Mayo²,

Anstey³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

129

115

View full text Add to dashboard Cite

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“…It is, therefore, likely that there is strain-to-strain variation in low temperature survival of B. pseudomallei . B. pseudomallei has been isolated from a non-endemic temperate location over a period of 25 years, 21 showing long-term survival in subtropical conditions where ground temperature can fall as low as 0°C in winter.…”

Section: Resultsmentioning

confidence: 99%

The Survival of Burkholderia pseudomallei in Liquid Media

Robertson¹,

Levy²,

Sagripanti³

et al. 2010

The American Society of Tropical Medicine and Hygiene

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We studied the effect of environmental parameters on the survival of Burkholderia pseudomallei. There was a small increase in bacterial count for up to 28 days in sterilized distilled water or rain water, in water at 20°C or 40°C, and in buffered solutions of pH 4 or higher. Counts of culturable B. pseudomallei declined at pH 3, in the presence of seawater or water with concentrations of 4% salt or higher, and under refrigeration. The morphological appearances of B. pseudomallei changed under conditions that maintained culturable numbers from bacilli to coccoid cells and spiral forms under pH or salt stress. These observations indicate that B. pseudomallei can endure nutrient-depleted environments as well as a wide range of pH, salt concentrations, and temperatures for periods of up to 28 days. The relative stability of B. pseudomallei under these conditions underlines the tenacity of this species and its potential for natural dispersal in water: in surface water collections, in managed water distribution systems, and through rainfall. These survival properties help explain the recent expansion of the known melioidosis endemic zone in Australia and may have played a part in recent melioidosis outbreaks.

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“…13 B. pseudomallei can also seed new endemic areas and cause long-term contamination of soil and water outside of the tropics. 14,15 Taken together, these findings suggest that this organism has many opportunities to disperse widely. However, an important factor likely to limit dispersal is the sensitivity of B. pseudomallei to ultraviolet radiation, 16 which has been exploited to eradicate the bacteria from unchlorinated bore water supplies.…”

mentioning

confidence: 79%

Burkholderia pseudomallei Genotype Distribution in the Northern Territory, Australia

Chapple

Price

Sarovich

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Melioidosis is a tropical disease of high mortality caused by the environmental bacterium, Burkholderia pseudomallei. We have collected clinical isolates from the highly endemic Northern Territory of Australia routinely since 1989, and animal and environmental B. pseudomallei isolates since 1991. Here we provide a complete record of all B. pseudomallei multilocus sequence types (STs) found in the Northern Territory to date, and distribution maps of the eight most common environmental STs. We observed surprisingly restricted geographic distributions of STs, which is contrary to previous reports suggesting widespread environmental dissemination of this bacterium. Our data suggest that B. pseudomallei from soil and water does not frequently disperse long distances following severe weather events or by migration of infected animals.

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Pseudomonas pseudomalleiisolates collected over 25 years from a non-tropical endemic focus show clonality on the basis of ribotyping

Cited by 69 publications

References 16 publications

A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates.

A cluster of melioidosis cases from an endemic region is clonal and is linked to the water supply using molecular typing of Burkholderia pseudomallei isolates.

The Survival of Burkholderia pseudomallei in Liquid Media

Burkholderia pseudomallei Genotype Distribution in the Northern Territory, Australia

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