Psoralea corylifolia L. Inhibits Mitochondrial Complex I and Proteasome Activities in SH‐SY5Y Cells

Abstract

The growing interest in alternative medicines, including traditional medicinal plants, has caused some health concerns due to poor awareness in the general population of the possible side effects from inappropriate practices. Psoralea corylifolia L. has been used in Chinese and Indian traditional medicine for the treatment of various inflammatory diseases of the skin and to improve vitality. Our data show that the extract obtained from the seeds of Psoralea corylifolia L. decreased mitochondrial complex I and proteasome activities; and oxidative stress might be an early event.

Ageing in nematodes: do antioxidants extend lifespan in Caenorhabditis elegans?

“…We acknowledge that the use of a marker of protein oxidative damage neglects possible protective effects on lipids and DNA, and that the levels of protein carbonyls are determined not only by the rate of oxidative damage but also by their repair/removal, the latter factor being largely independent of antioxidant action. It is, for instance, possible that some of the extracts reduce the clearance of oxidative damage via means such as proteasome inhibition (Tang et al 2007). Other factors to be taken into account include absorption, metabolism, compartmentalization and the specifics of the ROS and target molecules, all of which will affect antioxidant efficacy in vivo (Frankel and Finley 2008).…”

Inhibition of Human 20S Proteasome by Compounds from Seeds of Psoralea corylifolia

“…17 As mentioned in the above, there has been a report that the extract of Psoraleae Semen inhibited mitochondrial complex I and proteasome activities in SH-SY5Y, assuming that the interplay of oxidative damage due to reactive species generation, mitochondrial complex I inhibition, and proteasomal dysfunction may be responsible for the reduced cell viability in SH-SY5Y neuroblastoma cells treated with Psoraleae Semen, possibly leading to increase in the risk of development of neurodegenerative diseases such as Parkinson's and Alzheimer's diseases. 7 However, even though the inhibitory activity of P. corylifolia on proteasome has been reported before, 7 the compounds from the extract that may be responsible for the activity were identified in this study for the first time. Moreover, 20S proteasome purified from human erythrocyte was used to determine the chymotrypsin-like activity of proteasome in this study whereas proteasome-containing SH-SY5Y cells which were lysed with cell lysis buffer were used without purification in the above mentioned report.…”

“…7 However, the compounds that are responsible for its inhibitory activity have not been identified so far.…”

Targeting Tumor Proteasome with Traditional Chinese Medicine

“…This plant has been used in Chinese and Indian traditional medicine for the treatment of leukoderma, leprosy, psoriasis, vitiligo, and inflammatory diseases of the skin [68]. Recent animal studies suggest it potential use to prevent brain aging [69].…”

“…No monomer of Psoralea corylifolia L. has been reported to target the proteasome, while the whole extract of this medicinal plant was found to inhibit the proteasome [68]. Incubation of SH-SY5Y cells with 20 μg/mL of Psoralea corylifolia for 24 h significantly inhibited all the three proteasome activities to a similar level (about 20% lower compared to control).…”