“
            <i>Psychrodesulfovibrio</i>
            ” gen. nov.

Galushko, Alexander; Kuever, Jan

doi:10.1002/9781118960608.gbm01892

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…The addition of several new genera, namely Pseudodesulfovibrio ( Cao et al, 2016 ), Halodesulfovibrio ( Shivani et al, 2017 ), “Mailhella” ( Ndongo et al, 2017 ), Desulfohalovibrio , Desulfocurvibacter ( Spring et al, 2019 ), “ Paradesulfovibrio” ( Kim et al, 2020 ), and Desulfolutivibrio ( Thiel et al, 2020 ), finally led to a major reorganization based on the genome sequence data ( Waite et al, 2020 ), resulting in Desulfovibrionaceae currently comprising nine validly published genera ( Fundidesulfovibrio, Humidesulfovibrio, Maridesulfovibrio, Megalodesulfovibrio, Nitratidesulfovibrio, Oleidesulfovibrio, Paradesulfovibrio, Paucidesulfovibrio , and Solidesulfovibrio ) and three published but not validated genera ( “Alteridesulfovibrio,” “Aminidesulfovibrio,” and “Frigididesulfovibrio” ) besides the existing genera. Reorganizing the confusing taxonomy of the Desulfovibrionaceae family was completed by adding two valid [ Macrodesulfovibrio ( Galushko and Kuever, 2020b ), Oceanidesulfovibrio ( Galushko and Kuever, 2020c )] and one invalid genera [“ Psychrodesulfovibrio ” ( Galushko and Kuever, 2020d )] at the end of 2020 through Bergey’s manual ( Galushko and Kuever, 2020a ). As of August 2021, the Desulfovibrionaceae family comprised 21 validated genera, one synonym, and five non-validated genera.…”

Section: Introductionmentioning

confidence: 99%

Establishment of Genome Based Criteria for Classification of the Family Desulfovibrionaceae and Proposal of Two Novel Genera, Alkalidesulfovibrio gen. nov. and Salidesulfovibrio gen. nov.

et al. 2022

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Bacteria in the Desulfovibrionaceae family, which contribute to S element turnover as sulfate-reducing bacteria (SRB) and disproportionation of partially oxidized sulfoxy anions, have been extensively investigated since the importance of the sulfur cycle emerged. Novel species belonging to this taxon are frequently reported, because they exist in various environments and are easy to culture using established methods. Due to the rapid expansion of the taxon, correction and reclassification have been conducted. The development of high-throughput sequencing facilitated rapid expansion of genome sequence database. Genome-based criteria, based on these databases, proved to be potential classification standard by overcoming the limitations of 16S rRNA-based phylogeny. Although standards methods for taxogenomics are being established, the addition of a novel genus requires extensive calculations with taxa, including many species, such as Desulfovibrionaceae. Thus, the genome-based criteria for classification of Desulfovibrionaceae were established and validated in this study. The average amino-acid identity (AAI) cut-off value, 63.43 ± 0.01, was calculated to be an appropriate criterion for genus delineation of the family Desulfovibrionaceae. By applying the AAI cut-off value, 88 genomes of the Desulfovibrionaceae were divided into 27 genera, which follows the core gene phylogeny results. In this process, two novel genera (Alkalidesulfovibrio and Salidesulfovibrio) and one former invalid genus (“Psychrodesulfovibrio”) were officially proposed. Further, by applying the 95–96% average nucleotide identity (ANI) standard and the 70% digital DNA–DNA hybridization standard values for species delineation of strains that were classified as the same species, five strains have the potential to be newly classified. After verifying that the classification was appropriately performed through relative synonymous codon usage analysis, common characteristics were listed by group. In addition, by detecting metal resistance related genes via in silico analysis, it was confirmed that most strains display metal tolerance.

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