Pterodon polygalaeflorusEssential Oil Modulates Acute Inflammation and B and T Lymphocyte Activation

Abstract

The increased life expectancy of the population has led to increasing incidences of cancer, chronic inflammatory and autoimmune diseases. Thus the continuous search for new drugs is necessary because ineffectiveness and adverse effects have been described for standard drugs. Essential oils are important sources of bioactive metabolites and several clinical trials have been developed using them. The Pterodon genus has been used in traditional medicine to treat rheumatic disorders, thus this work investigated the properties of essential oil from Pterodon polygalaeflorus fruits (EsOPpg) on acute inflammation and lymphocyte activation. The essential oil was obtained by hydrodistillation and its components were identified by GC/MS. The anti-inflammatory response was assessed using the air pouch model. Antinociceptive potential was evaluated using the writhing model. Lymphocyte phenotyping, cell cycle and apoptosis were analyzed by flow cytometry. EsOPpg promoted a reduction in leukocyte counts and protein concentration in the exudate, and reduced vasodilatation and inflammatory cell infiltrate in air pouch tissue. No antinociceptive effect was demonstrated for the doses tested. EsOPpg inhibited lymphocyte proliferation, arresting the cell cycle in G1 phase, and induced apoptosis in these cells. EsOPpg downregulated both the total number of CD8(+) T cells and the activated subpopulation (CD8(+)CD69(+)), while promoting upregulation of the total number of CD19(+) and CD19(+)CD69(+) B cells. In conclusion, Pterodon polygalaeflorus essential oil diminished the acute inflammatory response and inhibited lymphocyte proliferation, reducing neutrophil recruitment into the cavity and air pouch tissue and promoting distinct modulations of the activation level of each lymphocyte subpopulation.

Flavonoid-enriched fraction from Echinodorus macrophyllus aqueous extract exhibits high in-vitro and in-vivo anti-inflammatory activity

“…Carrageenan injection in the air pouch also induces high leucocyte migration into the cavity, as showed here in the vehicle group. This leucocyte recruitment represents one of the mechanisms supporting primary host defence, although it can also cause tissue damage if the response is not controlled.…”

Essential oil of Pterodon polygalaeflorus Benth attenuates nociception in mice

“…Velozo et al (31) showed that P. polygalaeflorus has anti-inflammatory activity by modulation of B cells and lymphocyte activation. Due to the effect of EOPPgfl in the second phase of the formalin test and because of the medicinal use of P. polygalaeflorus seed extracts for the treatment of inflammatory diseases, we hypothesized that EOPPgfl acted by a mechanism that includes an indirect anti-inflammatory effect.…”

CHEMICAL COMPOSITION, LARVICIDAL ACTIVITY AND RESIDUAL EFFECT OF Pterodon polygalaeflorus (BENTH.) BENTH. (FABACEAE) FRUIT OIL EXTRACTS AGAINST Aedes aegypti (DIPTERA: CULICIDAE)

“…Among the main chemical constituents found in the species of the genus Pterodon are the vouacapane diterpenes, which cause different types of biological activities (Oliveira et al, 2017a). The oil extract of P. polygalaeflorus fruit showed antirheumatic activity (Hoscheid & Cardoso 2015); anti-inflammatory and nociceptive activities (Moraes et al, 2012; Coelho-de-Souza et al, 2018); antispasmodic activity (Leonhardt et al, 2010); Ca 2+ like channel blockers and Na + -dependent electromechanical coupling (Evangelista et al, 2007;Reis et al, 2015) as well as immune system modulators, interfering with cell migration and interleukin inhibition (Velozo et al, 2013, Alberti et al, 2014.…”