<i>PTR3</i>, a novel gene mediating amino acid‐inducible regulation of peptide transport in <i>Saccharomyces cerevisiae</i>

Barnes, David J.; Lai, Wing‐Fu; Breslav, Michael; Naider, Fred; Becker, Jeffrey M.

doi:10.1046/j.1365-2958.1998.00931.x

Cited by 35 publications

(28 citation statements)

References 35 publications

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“…Amino acid-induced and SPS-sensor-mediated activation of dipeptide transporter PTR2 expression is a requisite for dipeptide uptake (Island et al 1987;Barnes et al 1998;Hauser et al 2001); however, full induction of peptide uptake requires an additional activation step (Byrd et al 1998;Turner et al 2000;Hwang and Varshavsky 2008;Xia et al 2008). Peptides entering the cell that contain N-terminal amino acids recognized according to the N-end rule as destabilizing (Dohmen et al 1994;Varshavsky 2008), allosterically activate Ubr1, an E3 ubiquitin ligase, which in turn accelerates degradation of the transcriptional repressor Cup9.…”

Section: Pathway Genesmentioning

confidence: 99%

Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae

2012

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Ever since the beginning of biochemical analysis, yeast has been a pioneering model for studying the regulation of eukaryotic metabolism. During the last three decades, the combination of powerful yeast genetics and genome-wide approaches has led to a more integrated view of metabolic regulation. Multiple layers of regulation, from suprapathway control to individual gene responses, have been discovered. Constitutive and dedicated systems that are critical in sensing of the intra- and extracellular environment have been identified, and there is a growing awareness of their involvement in the highly regulated intracellular compartmentalization of proteins and metabolites. This review focuses on recent developments in the field of amino acid, nucleotide, and phosphate metabolism and provides illustrative examples of how yeast cells combine a variety of mechanisms to achieve coordinated regulation of multiple metabolic pathways. Importantly, common schemes have emerged, which reveal mechanisms conserved among various pathways, such as those involved in metabolite sensing and transcriptional regulation by noncoding RNAs or by metabolic intermediates. Thanks to the remarkable sophistication offered by the yeast experimental system, a picture of the intimate connections between the metabolomic and the transcriptome is becoming clear.

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Section: Pathway Genesmentioning

confidence: 99%

Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae

2012

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“…Six of the family members, including BAP2 and BAP3, comprise a subset of more related AAPs that are transcriptionally induced by extracellular amino acids. The amino-acid-induced expression of these permeases requires the SSY1, PTR3, and SSY5 gene products (Barnes et al 1998;Didion et al 1998;Jørgensen et al 1998;Iraqui et al 1999;Klasson et al 1999;Forsberg and Ljungdahl 2001a). Available data suggest that Ssy1p, Ptr3p, and Ssy5p function together as a plasmamembrane-localized sensor complex, dubbed the SPS sensor (Forsberg and Ljungdahl 2001a).…”

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confidence: 99%

Receptor-mediated endoproteolytic activation of two transcription factors in yeast

Andréasson¹,

Ljungdahl²

2002

Genes Dev.

113

165

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Yeast possess a plasma membrane sensor of external amino acids that functions as a ligand-activated receptor. This multimeric sensor, dubbed the SPS sensor, initiates signals that regulate the expression of genes required for proper amino acid uptake. Stp1p and Stp2p are transcription factors that bind to specific sequences within the promoters of SPS-sensor-regulated genes. These factors exhibit redundant and overlapping abilities to activate transcription. We have found that Stp1p and Stp2p are synthesized as latent cytoplasmic precursors. In response to extracellular amino acids, the SPS sensor induces the rapid endoproteolytic processing of Stp1p and Stp2p. The processing of Stp1p/Stp2p occurs independently of proteasome function and without the apparent involvement of additional components. The shorter forms of these transcription factors, lacking N-terminal inhibitory domains, are targeted to the nucleus, where they transactivate SPS-sensor target genes. These results define a completely unique and streamline metabolic control pathway that directly routes environmental signals initiated at the plasma membrane to transcriptional activation in the nucleus of yeast.

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“…Minimal poor nitrogen source (MP + ) and minimal rich nitrogen source (MR + ) media have also been previously described (Barnes et al, 1998). This minimal complete medium was made with 20 g glucose and 1.7 g yeast nitrogen base without amino acids and without ammonium sulphate (Difco) and 20 g agar (for solid media) per litre.…”

Section: Yeastmentioning

confidence: 99%

“…some SC media at 60 mM final concentration to score ptr3 mutants (Barnes et al, 1998). Rich media used to test rescue of ssy1 mutants contained extra leucine at 0.5 mg/l, 1.0 mg/l or 2.0 mg/l.…”

Section: Yeastmentioning

confidence: 99%

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Characterization of genes that are synthetically lethal with ade3 or leu2 in Saccharomyces cerevisiae

Nigavekar

Cannon

2001

Yeast

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Combinations of two non-lethal mutations that result in cell death are synthetically lethal. Such a genetic relationship suggests a functional interaction between the corresponding gene products. Frequently, an ade2 ade3 colony-sectoring assay is used to screen for synthetic lethal mutants. In these screens, mutants are sought that fail to lose a plasmid that bears a gene of interest. However, a subset of mutants is often found that is dependent on plasmid components other than the target gene. To understand the mechanism of this dependence, we characterized those mutants that, although prevalent in most mutant hunts, are usually discarded. Using a LEU2-ADE3 plasmid, plasmid-dependent mutations were found in the SHM2, PTR3, BAP2 and SSY1 genes. Double shm2 ade3 mutants are nonviable because the two pathways for tetrahydrofolate synthesis are blocked. Mutations in PTR3, BAP2 and SSY1 disrupt sensing and transport of extracellular leucine. Therefore, ptr3, bap2 or ssy1 mutants must be leucine prototrophs to grow on rich media. In light of these findings, we propose modifications that should improve the efficiency of synthetic lethal screening procedures.

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PTR3, a novel gene mediating amino acid‐inducible regulation of peptide transport in Saccharomyces cerevisiae

Cited by 35 publications

References 35 publications

Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae

Regulation of Amino Acid, Nucleotide, and Phosphate Metabolism in Saccharomyces cerevisiae

Receptor-mediated endoproteolytic activation of two transcription factors in yeast

Characterization of genes that are synthetically lethal with ade3 or leu2 in Saccharomyces cerevisiae

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