PUM1 and RNase P genes as potential cell‐free DNA markers in breast cancer

Abstract

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Cancer Biomarkers in Liquid Biopsy for Early Detection of Breast Cancer: A Systematic Review

“…As shown in Table 7, highlighted in gray, 16 biomarker panels in peripheral blood can be observed 19,22,27,29,30,42,44,54,61,81,93,98,106,111,112,123 and 2 biomarker panels in saliva 74,86 with a sensitivity greater than 90%.…”

“…In total, 16 142 articles were identified in the aforementioned databases (Table 1). However, 234 articles were retained for full reading, following the elimination of duplicates and articles whose title and abstract did not meet the eligibility criteria; of these, only 136 12…”

“…However, in 10 of the studies in which sensitivity was less than 70%, and in one, 80% greater specificity was reported. In contrast, 26 articles reported sensitivity and specificity figures greater than 70% for combined biomarkers in the form of panels 27,29,30,61,76,77,81,82,87,90,93,94,97,98,101,104,106,111-114,118,123,124,132,147 leaving only 6 panels with figures lower than 70%. 61,76,93,114,124,132 Finally, 27 articles reported AUC and P values, 26,62,63,67,71,72,78,83,84,88,102,110,116,120,122,125,127,130,133,135,137,139,140,142,143,145,146 and of these, AUC was lower than 0.7 67…”

Multiple structural flavors of RNase P in precursor tRNA processing

“…Given the many applications of circular RNA (circRNA) in translational research (Li, 2023;Sridhara & Sanyal, 2011;Zhou et al, 2020), one can envision developing novel ways to circularize RNA targets of choice. Apart from these applications, RNase P has been employed as a control biomarker for the detection of cell-free DNA towards the diagnosis of breast cancer (Murillo Carrasco et al, 2021) and as an internal control to normalize viral load during corona virus disease (COVID-19) diagnosis as a means to assess the extraction efficiency and specimen quality of RNA extracted from patient nasopharyngeal swab samples (Maidana-Kulesza et al, 2022;Sridhara et al, 2021). Interestingly, the RNA levels of RPP30 shared between RNase P and RNase MRP were shown to strongly correlate with positive diagnosis in SARS-CoV-2 detection tests, suggesting that it could be used as an internal reference to specifically identify false negative reverse transcription polymerase chain reaction (RT-PCR) results in COVID-19 diagnosis (Zhang et al, 2020a(Zhang et al, , 2020b.…”

Pumilio RNA binding family member 1 deficiency activates anti-tumor immunity in hepatocellular carcinoma via restraining M2 macrophage polarization

“…By binding targets speci cally on the mRNAs at the 3'UTR region, the Pumilio-binding elements (PBEs) control cell differentiation and fate determination [15][16][17]. Recent genetic research on the genes that encode the RNA-binding proteins known as Pumilio-1 (Pum1) and Pumilio-2 (Pum2), members of the mammalian PUF family, have shown the functions of post-transcriptionally controlling growth, reproduction, and development of the eukaryotic brain in addition to malignant tumors progression [18][19][20]. An earlier study revealed that PUM1 is overexpressed in colon cancer cells that have developed Cetuximab resistance and is associated with TAM [21][22].…”