Religion, Learning and Science in the 'Abbasid Period. M. J. L. Young , J. D. Latham , R. B. Serjeant

A three‐dimensional plot for the display of transverse pore gradient gel electrophoresis data: Application to a kinetoplast DNA fragment of planar circular conformation

“…No.0310N75IT). The gels were run in 0.5 X TBE (Tris-boric acid-EDTA) buffer at room temperature with a DNA load of 0.2 pg and a field strength of 10 V/cm as described previously [5]. Ethidium bromide-stained gels were video photographed using the GelPrint acquisition system and analyzed as described [6].…”

“…Ethidium bromide-stained gels were video photographed using the GelPrint acquisition system and analyzed as described [6]. Second-order polynomial functions were obtained for the acquired Ferguson curves [5]. The functions describe the data almost perfectly and allow one to interpolate between experimental values in order to generate a smooth, evenly spaced set of mobility data for each DNA fragment.…”

“…Moreover, the ease of applying the method is greatly improved by use of commercially available gels. The transverse pore gradient gel electrophoresis patterns can be evaluated semi-automatically by computer [5]. Their conversion into a three-dimensional plot allows the simultaneous display of all the information contained in the three variables of DNA length, gel concentration and DNA mobility.…”