Retracted: Antibodies to mycobacterial antigens for diagnosis of tuberculosis

Abstract

In spite of the advent of several newer modalities in molecular and immunological methods of diagnosis, the diagnosis of smear negative tuberculosis is a major problem even today. Use of antibody detection ELISA for the purpose has been evaluated by many authors using a variety of antigens derived from mycobacteria. These antigens are reported to have many advantages and disadvantages. Here we review commonly used antigens used for detection of antimycobacterial antibodies for diagnosis of tuberculosis. Each antigen is reviewed for its application in diagnosis, sensitivity, specificity, cross-reactivity and clinical application.

Mycobacterium tuberculosis Rv1419 encodes a secreted 13 kDa lectin with immunological reactivity during human tuberculosis

“…These observations suggest that sMTL-13-host interaction merits further investigation as a potential biomarker of diagnosis/treatment efficacy as well as immunization target. In this regard, it should be noted that secreted antigens are utilized as diagnostic tests as well as a vaccine candidates in current clinical trials [43,44].…”

Immunological Diagnosis of Active and Latent TB

“…Since enhancement of B cell immunity and production of antibody along with protective cell-mediated immunity may play an important role in the immunopathogenesis of tuberculosis, detection of specific antibodies against various mycobacterial derived antigens could also play a significant role in the diagnosis of tuberculosis. The value of various mycobacterial native or recombinant protein, lipid or different combinations of purified antigens or commercially available kits as a potential candidate of active tuberculosis sero-marker was evaluated by the ELISA method in many attempts (Abebe et al, 2007;Verma & Jain, 2007;Steingart et al, 2009). Development of a serological test with sufficient diagnostic efficacy for tuberculosis diagnosis could be very much useful tool in resource-limited countries, as the procedures are simple, relatively cost effective and can be performed rapidly.…”

“…Use of native or recombinant protein did not show any difference in term of diagnostic efficacy. But, the sensitivity of IgG detection was relatively higher than that of IgA against 38 kDa antigen (Verma & Jain, 2007). Several commercially available antibody detection kits were also developed using this antigen including Pathozyme Myco (LAM+38kDa), Pathozyme TB complex plus (38kDa+16kDa).…”

“…However, lower rate of sensitivity (50.5%) and comparable specificity (78.3%) for the diagnosis of pulmonary tuberculosis patients were reported by Tessema et al (2002). In addition, MycoDot, (Genelabs, Switzerland), a commercially available kit for the detection of antibodies against MTB specific LAM was also evaluated in many reports (Steingart et al, 2007;Verma & Jain, 2007). Although the specificities were high (84-100%), low rate of sensitivities (16-56%) were obtained and low sensitivities were mostly related to HIV/TB co-infection (Verma & Jain, 2007) DAT, TAT and SL-I: Assessment of antibody responses using DAT, TAT, and SL-I antigens in ELISA for serodiagnosis of tuberculosis revealed variable results in terms of diagnostic efficacy.…”

Comparación de adenosina deaminasa y detección de anticuerpos anti-antígeno A60 para el diagnóstico de meningitis tuberculosa

“…El rango de detección en LCR para diferentes antígenos de M. tuberculosis en pacientes cursando MTBC varía de 60 a 100% en las distintas series, con importantes diferencias entre los casos individuales, técnicas de detección de anticuerpos únicos o múltiples, momento de toma de muestra en el curso de la enfermedad, status VIH del paciente y método de definición del estándar de oro (diagnóstico clínico versus cultivo) [12][13][14][15] . El complejo A60 representa una fracción importante del total de antígenos de M. tuberculosis y la mayoría de los anticuerpos circulantes en sangre y LCR de pacientes cursando tuberculosis están dirigidos contra A60 16 . Sin embargo, el rendimiento diagnóstico de los anticuerpos www.sochinf.cl…”