<i>Retracted</i>: Baicalin relieves lipopolysaccharide‐evoked inflammatory injury through regulation of miR‐21 in H9c2 cells

Liu, Xiangyu; Wang, Shengli; Zhao, G J

doi:10.1002/ptr.6583

Cited by 16 publications

(14 citation statements)

References 35 publications

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“…Antimicrobial peptides (AvBD1, AvBD2, and AvBD4) and Lyz are important components of the intestinal chemical barrier, which can protect the intestinal mucosa from damage ( Fusco et al, 2021 ). In addition, multiple studies have found that BA can strengthen the intestinal barrier and maintain intestinal health by affecting multiple functions of the body, including alleviating the inflammatory response and enhancing antioxidant capacity ( Liu et al, 2020 ; Rizzo et al, 2021 ). Notably, the results of the present study showed that after APEC infection, the mRNA and/or protein levels of TJs, MUC2, AvBDs, and Lyz were markedly downregulated, indicating that the intestinal barrier had been destroyed; these results are consistent with Wu et al (2020) .…”

Section: Discussionmentioning

confidence: 99%

Baicalin ameliorates APEC-induced intestinal injury in chicks by inhibiting the PI3K/AKT-mediated NF-κB signaling pathway

Cheng¹,

Cao²,

Luo³

et al. 2022

Poultry Science

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Avian pathogenic Escherichia coli ( APEC ) is the causative agent of avian colibacillosis. Baicalin ( BA ) possesses multiple pharmacological effects, but the mechanism underlying its activity in APEC-induced intestinal injury remains unknown. This study aims to investigate the protective effects and possible mechanism of BA against APEC-induced intestinal injury. Sixty 1-day-old chicks were randomly divided into 4 groups: the control group (basal diet), E. coli group (basal diet), BAI10 group (10 mg/kg BA), and BAI20 group (20 mg/kg BA). After pretreatment with BA for 15 d and subsequent induction of APEC infection by pectoralis injection, the ileum was collected and analyzed. The results showed that BA-pretreatment demonstrated an alleviation of chicks in diarrhea rate, mortality, and histopathological changes in intestinal tissues after APEC infection. Additionally, following APEC infection, BA improved the intestinal barrier by elevating zona occludens ( ZO )s (ZO-1, 2, 3), Claudins (Claudin1, 2, 3), Occludin, avian β-defensin ( AvBD )s (AvBD1, 2, 4), lysozyme ( Lyz ) mRNA levels and ZO-1, Claudin1, and Occludin protein levels. Besides, the activities of total superoxide dismutase ( T-SOD ), catalase ( CAT ), and glutathione peroxidase ( GSH-Px ) and the SOD-1 and CAT mRNA levels and SOD-1 protein level were elevated by BA pretreatment. BA pretreatment also decreased the malondialdehyde ( MDA ) content, heme oxygenase-1 ( HO-1 ) and NADH quinone oxidoreductase 1 ( NQO1 ) mRNA levels, and HO-1 protein level after APEC infection. BA alleviated the APEC-induced inflammatory response, including downregulating the mRNA levels of proinflammatory cytokines (tumor necrosis factor-α ( TNF-α ), interleukin [ IL ]-1β, IL-6, IL-8) and upregulating the mRNA levels of anti-inflammatory cytokines (IL-4, IL-10, IL-13, transforming growth factor-β [ TGF-β ]). Furthermore, BA decreased the mRNA and protein levels of phosphatidylinositol 3 kinase ( PI3K ), protein kinase B ( AKT ), and nuclear factor kappa-B ( NF-κB ) as well as the expression of the phosphorylated forms of these proteins after APEC infection. Collectively, our findings indicate that BA exerts a protective effect against APEC-induced intestinal injury in chicks by inhibiting the PI3K/AKT-mediated NF-κB pathway, suggesting that BA may be a potential therapeutic approach for avian colibacillosis.

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Section: Discussionmentioning

confidence: 99%

Baicalin ameliorates APEC-induced intestinal injury in chicks by inhibiting the PI3K/AKT-mediated NF-κB signaling pathway

Cheng¹,

Cao²,

Luo³

et al. 2022

Poultry Science

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“…Baicalin (C21H18O11) is a flavonoid extracted from the natural plant Scutellaria baicalensis that has biological functions such as anti-inflammatory ( 21 ), anti-oxidant ( 22 ), and anti-viral effects ( 23 ). For example, baicalin can reverse LPS-induced inflammation in H9c2 cells ( 24 ). Baicalin exerted anti-oxidant effects against lung injury ( 25 ).…”

Section: Introductionmentioning

confidence: 99%

Baicalin Alleviates LPS-Induced Oxidative Stress via NF-κB and Nrf2–HO1 Signaling Pathways in IPEC-J2 Cells

et al. 2022

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Baicalin is a natural plant extract with anti-inflammatory and anti-oxidant activities. However, the molecular mechanism of baicalin on oxidative stress in IPEC-J2 cells exposed to LPS remains to be unclear. In this study, LPS stimulation significantly increased Toll-like receptor 4, tumor necrosis factor-α, and interleukins (IL-6 and IL-1β) expression in IPEC-J2 cells, and it activated the nuclear factor (NF-κB) expression. While, baicalin exerted anti-inflammatory effects by inhibiting NF-κB signaling pathway. LPS stimulation significantly increased the levels of the oxidative stress marker MDA, inhibited the anti-oxidant enzymes catalase and superoxide dismutase, which were all reversed by baicalin pre-treatment. It was found that baicalin treatment activated the nuclear import of nuclear factor-erythroid 2 related factor 2 (Nrf2) protein, and significantly increased the mRNA and protein expression of its downstream anti-oxidant factors such as heme oxygenase-1 and quinone oxidoreductase-1, which suggested that baicalin exerted anti-oxidant effects by activating the Nrf2-HO1 signaling pathway. Thus, pretreatment with baicalin inhibited LPS - induced oxidative stress and protected the normal physiological function of IPEC-J2 cells via NF-κB and Nrf2–HO1 signaling pathways.

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“…In particular, baicalin can effectively inhibit the growth of human herpes virus type 6 (Chen & Zhang, 2012 ). Baicalin has obvious therapeutic effects on viral myocarditis in humans, and its therapeutic mechanism may be related to miR21 (Liu et al., 2020 ). Baicalin can inhibit the activity of H 1 N 1 /H 3 N 2 influenza viruses in mice at the cellular level and in vivo (Ding et al., 2014 ) and its mechanism may involve the suppression of neuraminidase activity (Jin et al., 2018 ).…”

Section: Anti‐viral Effects Of Baicalinmentioning

confidence: 99%

Research progress on pharmacological effects and new dosage forms of baicalin

Bao

Liang

et al. 2022

Veterinary Medicine & Sci

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Background: As a kind of flavonoid, baicalin (C 21 H 18 O 11 ) is extracted from Scutellaria baicalensis Georgi, the extract of which can be added to animal feed in China. Objectives:The present review will describe the current understanding of the pharmacological effects of baicalin in the regulation of inflammation, oxidative stress anti-virus and anti-tumour responses. Methods:We highlight emerging literature that the application in livestock health and performance, the biological activities, the molecular mechanisms and the dosage forms of baicalin by analysing and summarising the main points of the cited literatures.Results: It is found that baicalin can improve the functions of multiple physiological systems. Baicalin has a strong anti-inflammatory effect by regulating TLR4-NFκB-MAPK signalling pathway; it also can reduce oxidative stress by regulating Nrf2-Keap1 pathway; it can inhabit many kinds of virus such as influenza virus, respiratory virus, hepacivirus and others; it can also inhibit the growth of tumour cells by blocking the cell cycle or inducing apoptosis; and new dosage forms such as cationic solid lipid nanoparticles, cyclodextrin inclusion complexes or nanocrystalline can be applied to improve the deficiency of baicalin. Conclusions:In summary, these studies have elucidated a comprehensive report on the anti-inflammatory, anti-oxidant, anti-virus and anti-tumour of baicalin, these findings thus indicated that baicalin can be used effectively to the field of animal production in future when the appropriate dosage form is determined. K E Y W O R D S anti-inflammatory, anti-oxidant, anti-tumour, anti-viral, baicalin Baicalin (C 21 H 18 O 11 ) is a flavonoid extracted from the dried roots of Scutellaria baicalensis Georgi. The species can be planted and produced in most provinces of northern China with relatively sufficient resources. Baicalin is the main active component of ScutellariaThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Retracted: Baicalin relieves lipopolysaccharide‐evoked inflammatory injury through regulation of miR‐21 in H9c2 cells

Cited by 16 publications

References 35 publications

Baicalin ameliorates APEC-induced intestinal injury in chicks by inhibiting the PI3K/AKT-mediated NF-κB signaling pathway

Baicalin ameliorates APEC-induced intestinal injury in chicks by inhibiting the PI3K/AKT-mediated NF-κB signaling pathway

Baicalin Alleviates LPS-Induced Oxidative Stress via NF-κB and Nrf2–HO1 Signaling Pathways in IPEC-J2 Cells

Research progress on pharmacological effects and new dosage forms of baicalin

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