<i>Retracted</i>: Cytosolic glyceraldehyde‐3‐phosphate dehydrogenase 2/5/6 increase drought tolerance via stomatal movement and reactive oxygen species scavenging in wheat

Zhang, Lin; Lei, Daili; Deng, Xia; Li, Fangfang; Ji, Haikun; Yang, Shushen

doi:10.1111/pce.13710

Cited by 17 publications

(5 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consistently, we found that WRKY33 exhibited a similar expression pattern to ATL31 and the expression levels of both genes were enhanced by Cd stress (Figures 1c,d, and 5g,h). Several studies have shown that WRKY33 acts not only as a positive regulator of salt tolerance (Jiang & Deyholos, 2009), thermo tolerance (Li et al, 2011), and systemic acquired resistance (Y. Wang et al, 2018), but as a negative regulator of SA accumulation and signalling as well (Birkenbihl et al, 2012; Zhang et al, 2019). Our results suggest that WRKY33 and ATL31 act synergistically by tightly controlling IRT1 homoeostasis in response to Cd stress and by regulating plant growth and development.…”

Section: Discussionmentioning

confidence: 99%

Regulatory module WRKY33‐ATL31‐IRT1 mediates cadmium tolerance in Arabidopsis

Zhang

Tong

Cao

et al. 2023

Plant Cell & Environment

View full text Add to dashboard Cite

Cadmium (Cd) is one of the most dangerous environmental pollutants among heavy metals, and threatens food safety and human health by accumulating in plant sink tissues.Here, we report a novel regulatory cascade that profoundly influences Cd tolerance in Arabidopsis. Phenotypic analysis showed that an insertional knockdown mutation at the Arabidopsis Tóxicos en Levadura 31 (ATL31) locus resulted in hypersensitivity to Cd stress, most likely due to a significant increase in Cd accumulation. Consistently, ATL31overexpressing lines exhibited enhanced Cd stress tolerance and reduced Cd accumulation. Further, IRON-REGULATED TRANSPORTER 1 (IRT1) was identified, and yeast twohybrid, co-immunoprecipitation and bimolecular fluorescence complementation assays demonstrated its interaction with ATL31. Biochemical, molecular, and genetic analyses showed that IRT1 is targeted by ATL31 for ubiquitin-conjugated degradation in response to Cd stress. Intriguingly, transcription of ATL31 was strongly induced by Cd stress. In addition, transgenic and molecular analyses showed that WRKY33 directly activated the transcription of ATL31 in response to Cd stress and positively regulated Cd tolerance.Genetic analysis indicated that ATL31 acts upstream of IRT1 and downstream of WRKY33 to regulate Cd tolerance. Our study revealed that the WRKY33-ATL31-IRT1 module plays a crucial role in timely blocking Cd absorption to prevent metal toxicity in Arabidopsis.

show abstract

Section: Discussionmentioning

confidence: 99%

Regulatory module WRKY33‐ATL31‐IRT1 mediates cadmium tolerance in Arabidopsis

Zhang

Tong

Cao

et al. 2023

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…Almost all abiotic stresses lead to the regulation of secondary oxidative stress stimulatory signals in a tissue-specific or even cell-specific manner ( Chaves and Oliveira, 2004 ). Increases in the corresponding enzyme activities under stress conditions protects plant cells from oxidative damage ( Chen et al, 2017 ; Zhang L. et al, 2020 ). To protect enzyme systems and membranes from lipid peroxidation, SOD, POD, and MDA activities increased at each growth stage after drought stress ( Gill and Tuteja, 2010 ; Abid et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Rehydration Compensation of Winter Wheat Is Mediated by Hormone Metabolism and De-Peroxidative Activities Under Field Conditions

et al. 2022

View full text Add to dashboard Cite

Water deficit and rehydration frequently occur during wheat cultivation. Previous investigations focused on the water deficit and many drought-responsive genes have been identified in winter wheat. However, the hormone-related metabolic responses and de-peroxidative activities associated with rehydration are largely unknown. In this study, leaves of two winter wheat cultivars, “Hengguan35” (HG, drought-tolerant cultivar) and “Shinong086” (SN, drought-sensitive cultivar), were used to investigate water deficit and the post-rehydration process. Rehydration significantly promoted wheat growth and postponed spike development. Quantifications of antioxidant enzymes, osmotic stress-related substances, and phytohormones revealed that rehydration alleviated the peroxidation and osmotic stress caused by water deficit in both cultivars. The wheat cultivar HG showed a better rehydration-compensation phenotype than SN. Phytohormones, including abscisic acid, gibberellin (GA), jasmonic acid (JA), and salicylic acid (SA), were detected using high-performance liquid chromatography and shown to be responsible for the rehydration process. A transcriptome analysis showed that differentially expressed genes related to rehydration were enriched in hormone metabolism- and de-peroxidative stress-related pathways. Suppression of genes associated with abscisic acid signaling transduction were much stronger in HG than in SN upon rehydration treatment. HG also kept a more balanced expression of genes involved in reactive oxygen species pathway than SN. In conclusion, we clarified the hormonal changes and transcriptional profiles of drought-resistant and -sensitive winter wheat cultivars in response to drought and rehydration, and we provided insights into the molecular processes involved in rehydration compensation.

show abstract

“…In the present study, sharp upregulation in glyceraldehyde-3-phosphate dehydrogenase involved in the transfer of glyceraldehyde-3-phosphate to 1, 3-bisphosphoglycerate, was only abundant above the CN in embryos of aged ST seeds (Table 1). Cytoplasmic glyceraldehyde-3-phosphate dehydrogenase can be induced under many stress conditions, such as anoxia and salt toxicity, and is involved in seed aging (Zhang et al, 2020). Cytoplasmic glucose-6-phosphate isomerase was also upregulated significantly only above the CN in embryos of aged ST seeds (Table 1).…”

Section: Maintaining the Carbon Homeostasis Might Be Essential For Longer Plateau Phase During Aamentioning

confidence: 99%

Comparative Proteomics at the Critical Node of Vigor Loss in Wheat Seeds Differing in Storability

et al. 2021

View full text Add to dashboard Cite

The critical node (CN, 85% germination) of seed viability is an important threshold for seed regeneration decisions after long-term conservation. Dependent on the germplasm, the storage period until CN is reached varies and information on the divergence of the proteomic profiles is limited. Therefore, the study aims to identify key proteins and mechanisms relevant for a long plateau phase and a late CN during artificial seed aging of wheat. Seeds of the storage-tolerant genotype (ST) TRI 23248, and the storage-sensitive genotype (SS) TRI 10230 were exposed to artificial ageing (AA) and extracted embryos of imbibed seeds were analyzed using an iTRAQ-based proteomic technique. ST and SS required AA for 24 and 18 days to reach the CN, respectively. Fifty-seven and 165 differentially abundant proteins (DAPs) were observed in the control and aged groups, respectively. Interestingly, a higher activity in metabolic processes, protein synthesis, transcription, cell growth/division, and signal transduction were already found in imbibed embryos of control ST seeds. After AA, 132 and 64 DAPs were accumulated in imbibed embryos of both aged ST and SS seeds, respectively, which were mainly associated with cell defense, rescue, and metabolism. Moreover, 78 DAPs of ST appeared before CN and were mainly enriched in biological pathways related to the maintenance of redox and carbon homeostasis and they presented a stronger protein translation ability. In contrast, in SS, only 3 DAPs appeared before CN and were enriched only in the structural constituents of the cytoskeleton. In conclusion, a longer span of plateau phase might be obtained in seeds when proteins indicate an intense stress response before CN and include the effective maintenance of cellular homeostasis, and avoidance of excess accumulation of cytotoxic compounds. Although key proteins, inherent factors and the precise regulatory mechanisms need to be further investigated, the found proteins may also have functional potential roles during long-term seed conservation.

show abstract

Retracted: Cytosolic glyceraldehyde‐3‐phosphate dehydrogenase 2/5/6 increase drought tolerance via stomatal movement and reactive oxygen species scavenging in wheat

Cited by 17 publications

References 84 publications

Regulatory module WRKY33‐ATL31‐IRT1 mediates cadmium tolerance in Arabidopsis

Regulatory module WRKY33‐ATL31‐IRT1 mediates cadmium tolerance in Arabidopsis

Rehydration Compensation of Winter Wheat Is Mediated by Hormone Metabolism and De-Peroxidative Activities Under Field Conditions

Comparative Proteomics at the Critical Node of Vigor Loss in Wheat Seeds Differing in Storability

Contact Info

Product

Resources

About