Retracted: Daidzin inhibits RANKL‐induced osteoclastogenesis in vitro and prevents LPS‐induced bone loss in vivo

Abstract

Osteoclasts are multinuclear giant cells responsible for bone resorption in bone loss diseases, including rheumatoid arthritis, periodontitis, and the aseptic loosening of orthopedic implants. Because of injurious side effects with currently available drugs, it is necessary to continue research novel bone‐protective therapies. Daidzin, a naturally occurring isoflavone found in leguminous plants, has numerous beneficial pharmacologic effects, including anti‐cancer, anti‐cholesterol, and anti‐angiocardiopathy, promoting osteoblasts differentiation, and even anti‐osteoporosis. However, the effect of daidzin on the regulation of osteoclast activity has not yet been investigated. In this study, our study showed that daidzin significantly inhibited receptor activator of nuclear factor‐kB ligand (RANKL)‐induced osteoclast differentiation of bone marrow macrophages and the hydroxyapatite‐resorbing activity of mature osteoclasts by inhibiting RANKL‐induced NF‐kB signaling pathway. In addition, daidzin could inhibit the expression of osteoclast marker genes, including nuclear factor of activated T cells cytoplasmic 1 (NFATc1), cellular oncogene fos (c‐Fos), tartrate‐resistant acid phosphatase (TRAP), and cathepsin K (CTSK). Consistent with in vitro results, daidzin inhibited lipopolysaccharide‐induced bone loss by suppressing the osteoclast differentiation. Together our data demonstrated that daidzin inhibits RANKL‐induced osteoclastogenesis through suppressing NF‐ĸB signaling pathway and that daidzin is a promising agent in the treatment of osteolytic diseases.

Corydalis Saxicola Bunting Total Alkaloids Attenuate Walker 256-Induced Bone Pain and Osteoclastogenesis by Suppressing RANKL-Induced NF-κB and c-Fos/NFATc1 Pathways in Rats

“…Once IκB is phosphorylated and degraded, the activated-NF-κB is transported to the nucleus, binding with the DNA site and then initiates the transcription of the target genes. Being similar to the previous reports (Ahn et al, 2008;Wei et al, 2019), nuclear accumulation of NF-κB (p50) was observed after sRANKL stimulation in this study. In contrast, CSBTA remarkably prevented nuclear accumulation of NF-κB (p50) and inhibited the phosphorylation of NF-κB (p65), thus suppressed formation of osteoclast (Figures 7A,B).…”

Bifidobacterium adolescentis improves lifespan and healthspan by regulating catalase activity and oxidative stress-associated metabolites

“…In addition, erucic acid, cosmosiin and 2hydroxycinnamic acid have been proposed as potential therapeutic agents for Alzheimer disease by ameliorating nueroin ammation and blocking neural cell death 27,29,37 . Daidzin could inhibit LPS-induced bone loss by suppressing the osteoclast differentiation 54 . The enrichment of these metabolites may underlie the improvement of phenotype in Terc −/− mice supplemented with B. adolescentis.…”

Assessment of the anti‐rheumatoid arthritis activity of Gastrodia elata (tian‐ma) and Radix aconitic lateralis preparata (fu‐zi) via network pharmacology and untargeted metabolomics analyses

“…The intake of linoleic acid can thereby induce anti‐RA activity 23‐25 . Daidzein and daidzin have been confirmed to suppress RA symptoms and decrease RA incidence 26‐29 . Hence, these compounds might play an important role in RA treatment.…”

“…[23][24][25] Daidzein and daidzin have been confirmed to suppress RA symptoms and decrease RA incidence. [26][27][28][29] Hence, these compounds might play an important role in RA treatment.…”