Retracted: Deep integrative analysis of microRNA‐mRNA regulatory networks for biomarker and target discovery in chondrosarcoma

Abstract

Chondrosarcoma (CHS) is a common malignant bone sarcoma and its occurrence increases with age. microRNAs (miRNAs) are a class of noncoding RNAs that participate in various biological processes and disease pathogenesis by targeting functional messenger RNA (mRNA). However, the modulation of miRNAs in CHS remains largely unknown. In this study, we performed integrative analysis to explore the expression profiles of miRNAs and mRNAs, together with their interaction networks in human CHS tissues and cell lines by RNA-seq (miRNA and mRNA). A total of 133 and 796 differentially expressed miRNAs and mRNAs were identified (|Fold change| ≥ 2 and P-value ≤ 0.5). miRNA-mRNA regulatory interactions between 55 miRNAs and 242 mRNAs were screened by the Pearson correlation analysis and target prediction. mRNAs in the network were enriched to 145 Gene Ontology terms and 35 Kyoto Encyclopedia of Genes and Genomes pathways. Specifically, some key regulators (hub-miRNAs) in the network (miR-622, miR-4539, miR-145, miR-25, and miR-96) were suggested to play important regulatory roles in the pathogenesis of CHS. In addition, functional experiments validated that miR-622 regulated CHS cell proliferation by targeting bone morphogenetic protein 1 (BMP1). K E Y W O R D S chondrosarcoma (CHS), miR-622, pearson correlation analysis, proliferation J Cell Biochem. 2019;120:9631-9638.wileyonlinelibrary.com/journal/jcb

The miR-143/145 Cluster, a Novel Diagnostic Biomarker in Chondrosarcoma, Acts as a Tumor Suppressor and Directly Inhibits Fascin-1

“…(37)(38)(39) Reduced levels of miR-143 or miR-145 have been reported in other tumor entities, and both molecules are generally acknowledged as tumor suppressors. (40,41) Our results are consistent with a recent publication showing miR-145 downregulation in chondrosarcoma (42) , however, the authors analyzed only a small cohort of five clinical samples without investigating miRNA functionality. Importantly, and in contrast to previous reports, our results are strengthened by an analysis of a considerably larger (42 samples) cohort.…”

Circulating extracellular vesicle‑encapsulated microRNA as screening biomarkers for intraductal papillary mucinous neoplasm

“…While we cannot find any relevant data regarding miR-6132 and tumors, the expression of miR-22 and miR-4539 in cancer tissue has been studied in gastric cancer, rhabdomyosarcoma, breast cancer, prostate cancer, osteosarcoma and papillary thyroid cancer (21)(22)(23)(24)(25)(26)(27)(28)(29)(30). Most studies have reported that, in contrast to their expression levels in the blood, the expression levels of these miRNAs are decreased in cancer tissue.…”

Comprehensive analysis of transcriptome data for identifying biomarkers and therapeutic targets in head and neck squamous cell carcinoma

“…Another study found out candidate miRNA-mRNA network that could be utilized to predict chemoresistance response in osteosarcoma (13). Moreover, a study provided a meaningful characterization of miRNA-mRNA co-expression network in chondrosarcoma and identified crucial miRNAs and mRNAs involved in chondrosarcoma carcinogenesis (14).…”