<i>Retracted</i>: In vivo and in vitro effects of microRNA‐124 on human gastric cancer by targeting JAG1 through the Notch signaling pathway

Xiao, Haijuan; Ji, Qing; Yang, Lin; Li, Renting; Zhang, Cheng; Hou, Jianquan

doi:10.1002/jcb.26413

Cited by 24 publications

(21 citation statements)

References 32 publications

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“…Hence, THY1 was selected as the main target of the present study. Existing studies have demonstrated the close correlation of THY1 gene with the Notch signaling pathway [26,27], with some even claiming the involvement of the Notch signaling pathway in GC [28,29]. On the basis of these studies, our team of researchers hypothesized the participation of THY1 in GC by mediating the Notch signaling pathway.…”

Section: Resultsmentioning

confidence: 97%

MicroRNA-140-5p inhibits cell proliferation, migration and promotes cell apoptosis in gastric cancer through the negative regulation of THY1-mediated Notch signaling

Zhang

Lin

et al. 2019

Bioscience Reports

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Studies have highlighted the importance of microRNAs (miRs) in the development of various cancers, including gastric cancer (GC), a commonly occurring malignancy, accompanied by high recurrence and metastasis rate. The aim of the current study was to investigate the role of miR-140-5p in GC. Microarray expression profiles were initially employed to screen the differentially expressed gene related to GC, and the miR regulating the gene was predicted accordingly. The data obtained indicated that thymus cell antigen 1 (THY1) was differentially expressed in GC and confirmed to be a target gene of miR-140-5p. Poorly expressed miR-140-5p and highly expressed THY1 were observed in the GC tissues. SGC-7901 cells were treated with miR-140-5p mimic/inhibitor, siRNA against THY1 and siRNA against Notch1 in order to determine their regulatory roles in GC cell activities. The relationship of miR-140-5p, THY1 and the Notch signaling pathway was subsequently identified. Moreover, cell proliferation, migration, invasion and apoptosis were determined using 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS), wound-healing, transwell assay and flow cytometry, respectively. The overexpression of miR-140-5p and silencing of THY1 resulted in a diminished expression of the Notch signaling pathway-related proteins, as well as inhibited proliferation, migration and invasion of GC cells, enhanced expression of pro-apoptotic proteins in addition to elevated apoptosis rate. Taken together, the present study suggests that miR-140-5p directly targets and negatively regulates THY1 expression and inhibits activation of the Notch signaling pathway, whereby the up-regulation of miR-140-5p inhibits development of GC, highlighting the promise of miR-140-5p as a potential target for GC treatment.

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Section: Resultsmentioning

confidence: 97%

MicroRNA-140-5p inhibits cell proliferation, migration and promotes cell apoptosis in gastric cancer through the negative regulation of THY1-mediated Notch signaling

Zhang

Lin

et al. 2019

Bioscience Reports

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“…Various literatures have reported that Notch pathway plays a vital part in GC metastasis and stemness. [13][14][15] Expression level of circ-NOTCH1 which derived from NOTCH1 and its role in GC metastasis and stemness through the regulation of Notch pathway are unclear. Therefore, this study mainly probed the role and mechanism of circ-NOTCH1 in cell metastasis and stemness of GC.…”

Section: Circ-notch1 Expression Was Conspicuously High In Gc Cell Lmentioning

confidence: 99%

miR‐449c‐5p availability is antagonized by circ‐NOTCH1 for MYC‐induced NOTCH1 upregulation as well as tumor metastasis and stemness in gastric cancer

Zhao

Zhong

Xue-yan

et al. 2020

J of Cellular Biochemistry

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Gastric cancer (GC), identified as the most common gastrointestinal malignancy, is one of the primary causes of cancer‐related mortality in the world. Although surgery and chemotherapy for GC treatment have been improved, the 5‐year overall survival rate is still unsatisfactory. Circ‐NOTCH1 is a novel circular RNA derived from its host gene NOTCH1, and has not been studied in any cancers. Here we explored the potential role and mediatory mechanism of circ‐NOTCH1 in GC. In this study, circ‐NOTCH1 exhibited increased expression in GC tissues and cells. Suppression of circ‐NOTCH1 inhibited cell migration, invasion, tumor spheroids number, and side population ratio. Circ‐NOTCH1 also promoted GC growth and metastasis in vivo. Additionally, it was found that circ‐NOTCH1 could bind to miR‐449c‐5p. Circ‐NOTCH1 promoted metastasis and stemness in GC through sponging miR‐449c‐5p. Subsequently, MYC was identified as a downstream gene of miR‐449c‐5p. MYC could bind to the promoter of NOTCH1 to regulate GC progression. Furthermore, rescue assays demonstrated that NOTCH1 knockdown reversed the effects of overexpression of MYC in metastasis and stemness in AGS cells/sh‐circNOTCH1. Above findings explained that circ‐NOTCH1 promoted metastasis and stemness in GC by targeting miR‐449c‐5p/MYC/NOTCH1 axis, suggesting the possibility of circ‐NOTCH1 as a therapeutic marker for GC.

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“…Precisely, an investigation conducted by Xiao et al, demonstrated that miR-124 targeting JAG1 suppress the Notch signaling pathway inhibiting invasion, migration, and proliferation of GC cells. [86].…”

Section: Micrornas and Gastric Cancer Stem Cellsmentioning

confidence: 99%

The Role of MicroRNAs in the Regulation of Gastric Cancer Stem Cells: A Meta-Analysis of the Current Status

Ruggieri

Russi

Zoppoli

et al. 2019

JCM

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Gastric cancer (GC) remains one of the major causes of cancer-related mortality worldwide. As for other types of cancers, several limitations to the success of current therapeutic GC treatments may be due to cancer drug resistance that leads to tumor recurrence and metastasis. Increasing evidence suggests that cancer stem cells (CSCs) are among the major causative factors of cancer treatment failure. The research of molecular CSC mechanisms and the regulation of their properties have been intensively studied. To date, molecular gastric cancer stem cell (GCSC) characterization remains largely incomplete. Among the GCSC-targeting approaches to overcome tumor progression, recent studies have focused their attention on microRNA (miRNA). The miRNAs are short non-coding RNAs which play an important role in the regulation of numerous cellular processes through the modulation of their target gene expression. In this review, we summarize and discuss recent findings on the role of miRNAs in GCSC regulation. In addition, we perform a meta-analysis aimed to identify novel miRNAs involved in GCSC homeostasis.

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Retracted: In vivo and in vitro effects of microRNA‐124 on human gastric cancer by targeting JAG1 through the Notch signaling pathway

Cited by 24 publications

References 32 publications

MicroRNA-140-5p inhibits cell proliferation, migration and promotes cell apoptosis in gastric cancer through the negative regulation of THY1-mediated Notch signaling

MicroRNA-140-5p inhibits cell proliferation, migration and promotes cell apoptosis in gastric cancer through the negative regulation of THY1-mediated Notch signaling

miR‐449c‐5p availability is antagonized by circ‐NOTCH1 for MYC‐induced NOTCH1 upregulation as well as tumor metastasis and stemness in gastric cancer

The Role of MicroRNAs in the Regulation of Gastric Cancer Stem Cells: A Meta-Analysis of the Current Status

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