<i>Retracted</i>: LncRNA HAND2‐AS1 exerts anti‐oncogenic effects on ovarian cancer via restoration of BCL2L11 as a sponge of microRNA‐340‐5p

Chen, Jun; Yang, Lin; Jia, Yan; Xu, Tianmin; Wu, Fengyuan; Jin, Yishuai

doi:10.1002/jcp.28911

Cited by 60 publications

(39 citation statements)

References 25 publications

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“…Luo and colleagues found that HAND2-AS1 expression decreased in hepatocellular carcinoma and related to metastasis [20]. Growing evidence revealed that HAND2-AS1 suppressed tumor progression via multiple signaling pathways in various cancers, including endometrioid endometrial carcinoma [21,22], head and neck squamous cell carcinoma [23], osteosarcoma [24,25], colorectal cancer [26,27], non-small cell lung cancer [28], esophagus squamous cell carcinoma [29], ovarian cancer [30], melanoma [31], and cervical squamous cell carcinoma [32]. The role of HAND2-AS1 in bladder cancer progression is unclear yet.…”

Section: Discussionmentioning

confidence: 99%

LncRNA HAND2-AS1 Exerts Anti-Oncogenic Effects on Bladder Cancer via Restoration of RARB as A Sponge of microRNA-146

Shan

Liu

Zhan

2020

Preprint

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Background Growing evidence has shown that the association of long noncoding RNA/microRNA/mRNA is implicated in tumor initiation, development, and progression. Long noncoding RNA (lncRNA) HAND2-AS1 exhibits anti-cancer effects in diverse cancers. However, the knowledge of HAND-AS1 in bladder cancer development remains unknown. Methods LncRNA and miRNA microarray were conducted to explore different expressed RNA in primary bladder cancer specimens. RNA-RNA interaction prediction tools miRcode (http://www.mircode.org/), DIANA-lncBase v2 (https://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=lncbasev2%2Findex-experimental), DIANA-TarBase v.8 (https://carolina.imis.athena-innovation.gr/diana_tools/web/index.php?r=tarbasev8%2Findex) and miRDB (http://www.mirdb.org/) were employed to predict the interactions between RNA. Bladder cancer cell lines were used to perform cell proliferation and apoptosis assays. Western blot and quantitative Real-time Polymerase Chain Reaction were used to determine the expression of protein and RNA, separately. Dual luciferase assay was conducted to determine the activity of 3’untranslational region of RARB. Furthermore, 5637 human bladder cancer mouse models were established to investigate the interactions of lncRNA: miRNA: mRNA in vivo.Results Based on the analysis of RT2 lncRNA PCR Arrays, we validated HAND2-AS1 declined in bladder cancer and correlated with the depth of invasion and grades negatively. Overexpression of HAND2-AS1 in human bladder cancer cells 5637 and RT4 hampered cell proliferation by provoking Caspase 3-triggered cell apoptosis. Besides, one of the HAND2-AS1 sponge, miR-146, upregulated in bladder cancer and targeted the tumor suppressor, retinoic acid receptor beta (RARB). We further demonstrated the HAND2-AS1: miR-146: RARB complex promoted Caspase 3-mediated apoptosis by suppressing COX-2 expression. Finally, results observed in mouse xenografts suggested that HAND2-AS1 diminished miR-146 expression, thereby reversing the suppression of miR-146 on RARB-mediated apoptosis and contributing to bladder cancer regression. Conclusion The present study shed light on the fact that lncRNA HAND2-AS1 exerted as a tumor suppressor by releasing RARB from miR-146, leading to inhibition of tumor proliferation and invasion. The findings expanded the knowledge of HAND2-AS-mediated regulatory networks, provided novel insights to improve the RARB-targeted regimens against bladder cancer.

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Section: Discussionmentioning

confidence: 99%

LncRNA HAND2-AS1 Exerts Anti-Oncogenic Effects on Bladder Cancer via Restoration of RARB as A Sponge of microRNA-146

Shan

Liu

Zhan

2020

Preprint

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“…Mounting researches indicated that lncRNA acted as a sponge for miRNA [26][27][28]. In the current study, 19 miRNA was selected out on condition that it can both bind to HCG11 and CTNNB1.…”

Section: Discussionmentioning

confidence: 98%

LncRNA HCG11 promotes proliferation and migration in gastric cancer via targeting miR-1276/CTNNB1 and activating Wnt signaling pathway

Zhang

Huang

et al. 2019

Cancer Cell Int

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Background: Gastric cancer (GC) is one common cancer which occurs in the stomach leading to high mortality around the world. Long non-coding RNAs (lncRNAs) were found overexpressed or silenced in the occurrence and progression of multiple cancers including GC. Method: The gene expression level in GC tissues and cells were analyzed by RT-qPCR. CCK-8, colony formation, flow cytometry and transwell assays were performed for the function analysis of HLA complex group 11 (HCG11). The mechanism study for HCG11 was conducted using RIP, RNA pull down and luciferase reporter assays. Results: HCG11 was discovered highly expressed in GC tissues and cells. Depletion experiments were used to evaluate HCG11 silence on cell proliferation, migration and apoptosis. Moreover, Wnt signaling pathway was found as a tumor promoter in GC. RIP assay, RNA pull down assay and luciferase reporter assay were performed to illustrate the relationship of HCG11, miR-1276 and CTNNB1. Rescue assays revealed that HCG11/miR-1276/CTNNB1 axis regulated the incidence and development of GC. Tumor formation in mice proved that HCG11 was negatively correlated with miR-1276 and had positively correlation with CTNNB1. Conclusion: Overall, HCG11 accelerated proliferation and migration in GC through miR-1276/CTNNB1 and Wnt signaling pathway, revealing that HCG11 could be a brand new target for GC.

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“…LncRNA WDFY3-AS2 acts as a tumor suppressor to inhibit tumor growth in OC via delaying miR-18a [23]. LncRNA HAND2-AS1 represents antioncogenic property in OC by targeting BCL2L11 [24]. This study was to explore the function of PAXIP1-AS1 in OC.…”

Section: Discussionmentioning

confidence: 99%

H3K27ac-induced lncRNA PAXIP1-AS1 exhibits oncogenic property in ovarian cancer by targeting miR-6744-5p/PCBP2 axis

Zheng¹

2020

Preprint

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We aimed to explore role of lncRNA PAX-interacting protein 1- antisense RNA1(PAXIP1-AS1) in ovarian cancer (OC). RT-qPCR analysis identified upregulation of PAXIP1-AS1 in OC cell lines. Functionally, PAXIP1-AS1 knockdown inhibited cell proliferation, accelerated cell apoptosis, and suppressed cell migration and epithelial-mesenchymal transition (EMT) process. Upregulation PAXIP1-AS1 was induced by CBP-mediated H3K27 acetylation (H3K27ac) via bioinformatic analysis and ChIP assay. Furthermore, PAXIP1-AS1 served as a competing endogenous RNA (ceRNA) to regulate PCBP2 expression by sponging microRNA-6744-5p (miR-6744-5p). Restoration experiments showed that overexpressed PCBP2 rescued effect of silenced PAXIP1-AS1 on cell proliferation, apoptosis, migration and EMT. Overall, lncRNA PAXIP1-AS1 activated by H3K27ac functioned as a tumor promoter in OC via mediating miR-6744-5p/PCBP2 axis, which provided promising insight into exploration on OC therapy.

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Retracted: LncRNA HAND2‐AS1 exerts anti‐oncogenic effects on ovarian cancer via restoration of BCL2L11 as a sponge of microRNA‐340‐5p

Cited by 60 publications

References 25 publications

LncRNA HAND2-AS1 Exerts Anti-Oncogenic Effects on Bladder Cancer via Restoration of RARB as A Sponge of microRNA-146

LncRNA HAND2-AS1 Exerts Anti-Oncogenic Effects on Bladder Cancer via Restoration of RARB as A Sponge of microRNA-146

LncRNA HCG11 promotes proliferation and migration in gastric cancer via targeting miR-1276/CTNNB1 and activating Wnt signaling pathway

H3K27ac-induced lncRNA PAXIP1-AS1 exhibits oncogenic property in ovarian cancer by targeting miR-6744-5p/PCBP2 axis

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