<i>Retracted:</i> Quercetin alleviates lipopolysaccharide‐induced inflammatory responses by up‐regulation miR‐124 in human renal tubular epithelial cell line HK‐2

Guo, Shuxia; Sun, Juanjuan; Zhuang, Yan

doi:10.1002/biof.1596

Cited by 19 publications

(13 citation statements)

References 41 publications

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“…Quercetin is reported to modulate the transport activities of the multidrug-resistance-associated proteins. Moreover, quercetin could not only influence the bioavailability of anticancer and antiviral drugs in vivo but also alleviate endotoxin-stimulated apoptosis and inflammation by up-regulation of relevant micro-RNA [41,42]. Additionally, quercetin could mitigate the intracellular formation of reactive oxygen species (ROS) to reduce the pro-inflammatory response [43].…”

Section: Discussionmentioning

confidence: 99%

Can network pharmacology identify the anti-virus and anti- inflammatory activities of Shuanghuanglian oral liquid used in Chinese medicine for respiratory tract infection?

Zhuang

Wen

Zhang

et al. 2020

European Journal of Integrative Medicine

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Introduction: Shuanghuanglian (SHL) oral liquid is a well-known traditional Chinese medicine preparation administered for respiratory tract infections in China. However, the underlying pharmacological mechanisms remain unclear. The present study aims to determine the potential pharmacological mechanisms of SHL oral liquid based on network pharmacology. Methods: A network pharmacology-based strategy including collection and analysis of putative compounds and target genes, network construction, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and Gene Ontology (GO) enrichment, identification of key compounds and target genes, and molecule docking was performed in this study. Results: A total of 82 bioactive compounds and 226 putative target genes of SHL oral liquid were collected. Of note, 28 hub target genes including 4 major hub target genes: estrogen receptor 1 (ESR1), nuclear receptor coactivator 2 (NCOA2), nuclear receptor coactivator 1 (NCOA1), androgen receptor (AR) and 5 key compounds (quercetin, luteolin, baicalein, kaempferol and wogonin) were identified based on network analysis. The hub target genes mainly enriched in pathways including PI3K-Akt signaling pathway, human cytomegalovirus infection, and human papillomavirus infection, which could be the underlying pharmacological mechanisms of SHL oral liquid for treating diseases. Moreover, the key compounds had great molecule docking binding affinity with the major hub target genes. Conclusion: Using network pharmacology analysis, SHL oral liquid was found to contain anti-virus, anti-inflammatory, and "multi-compounds and multi-targets" with therapeutic actions. These findings may provide a valuable direction for further clinical application and research. 1. Background SHL oral liquid is a well-known traditional Chinese medicine preparation frequently applied to treat acute upper respiratory tract infection, acute bronchitis, and pneumonia [1]. SHL oral liquid is derived from three Chinese medicinal herbs, namely, Flos Lonicerae (Jinyinhua, JYH), Radix Scutellariae (Huangqin, HQ), and Fructus Forsythiae (Lianqiao, LQ), which were officially recorded in the Chinese

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Section: Discussionmentioning

confidence: 99%

Can network pharmacology identify the anti-virus and anti- inflammatory activities of Shuanghuanglian oral liquid used in Chinese medicine for respiratory tract infection?

Zhuang

Wen

Zhang

et al. 2020

European Journal of Integrative Medicine

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“…Quercetin supplementation had been shown to attenuate D-Galactose-induced renal damage in rats and aflatoxin B1-induced neurotoxicity in mice through the inhibition of oxidative stress (20,21). A recent study also showed that quercetin supplementation could inhibit lipopolysaccharide (LPS)-induced inflammation in a proximal tubular cell line human kidney 2 (HK-2) cells which derived from normal human kidney (22). Yet, whether quercetin may have a protective role in Cu overload-induced nephrotoxicity remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms Underlying Protective Role of Quercetin on Copper Sulfate-Induced Nephrotoxicity in Mice

et al. 2021

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Copper overload is an established cause of nephrotoxicity, but the precise molecular mechanism remains unknown. Our study aimed to investigate the molecular mechanism of copper sulfate (CuSO4)-induced nephrotoxicity and the protective effect of the natural compound quercetin using a mouse model. Mice were orally administered CuSO4 only (200 mg/kg per day), or co-administered CuSO4 (200 mg/kg per day) plus quercetin (25, 50, or 100 mg/kg per day), or quercetin only (100 mg/kg per day), or vehicle for 28 days. The blood and kidneys were collected for the examination of serum biomarkers, oxidative stress biomarkers, changes in histopathology and gene and protein expression. Our results show that quercetin supplementation attenuates CuSO4-induced renal dysfunction and tubular necrosis in a dose-dependent manner. Quercetin supplementation at 50 and 100 mg/kg significantly attenuated CuSO4-induced oxidative damage. Quercetin supplementation also inhibited the activities of caspases-9 and−3, and the expression of p53 and Bax mRNAs. Furthermore, quercetin supplementation markedly activated the expression of Nrf2 and HO-1 mRNAs, but inhibited the expression of NF-κB, IL-1β, IL-6, and TNF-α mRNAs. In conclusion, our results revealed that quercetin supplementation could inhibit CuSO4-induced nephrotoxicity in mice via the inhibition of mitochondrial apoptotic and NF-κB pathways and the activation of Nrf2/HO-1 pathway. Our study highlights quercetin as a potential candidate in treating copper overload-induced nephrotoxicity.

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“…Moreover, a number of miRNAs have been found to directly target MyD88 upon LPS stimulation, including miR-149, hsa-miR-203, miR-214 (Cui et al, 2019;Zheng et al, 2019). Hsa-miR-203 is reported to be involved in the regulation of inflammatory responses in ischemia (Chen et al, 2017). Hsa-miR-203 was also involved in regulating LPS-induced inflammation in macrophage RAW264.7 cells (Wei et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

HSA-MIR-203/MyD88 axis mediates the protective effect of hispidulin on LPS-induced apoptosis in a human renal tubular epithelial line, HK-2

WANG¹,

LIU²,

Han³

et al. 2022

Biocell

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Acute kidney injury (AKI), commonly occurring as complications of sepsis, cardiac surgery, and liver or kidney transplantation, is a critical care syndrome. It is well known that lipopolysaccharide (LPS) shock is a common triggering factor for AKI. This study is aimed to examine the effect of flavonoid compound hispidulin on LPS-induced AKI. For this, renal tubular epithelial cell HK-2 was treated with LPS to establish an in vitro model of AKI. The effect of hispidulin on HK-2 cell viability was examined using CCK-8 assay. Cell apoptosis was determined by TUNEL and flow cytometry. Apoptosis marker proteins were determined by using western blot. The levels of pro-inflammatory cytokines were determined by ELISA assay and qRT-PCR. The translocation of NF-κB was determined by western blot. The effect of MyD88 on the cytoprotective activities of hispidulin was examined by overexpressing MyD88 in HK-2 cells. Our results showed that hispidulin was not able to produce a cytotoxic effect on HK-2 cells at tested concentrations.However, hispidulin could protect HK-2 cells from LPS-induced cell injury. Our results also showed that hispidulin was able to attenuate LPS-induced HK-2 cell apoptosis. In addition, LPS led to an inflammatory response in HK-2 cells, evidenced by NF-κB p65 activation as well as increased expression and release of inflammatory cytokine IL-6 and TNFα, which could be reversed by pretreatment with hispidulin. Overexpression MyD88 was found to significantly dampen the cytoprotective activities of hispidulin against LPS insult. More importantly, MyD88 was identified as a direct target of hsa-miR-203, and hispidulin was found to regulate the expression of MyD88 via upregulating hsa-miR-203. Our results showed that hispidulin attenuates LPS-induced HK-2 damage via regulating hsa-miR-203/MyD88 axis.

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Retracted: Quercetin alleviates lipopolysaccharide‐induced inflammatory responses by up‐regulation miR‐124 in human renal tubular epithelial cell line HK‐2

Cited by 19 publications

References 41 publications

Can network pharmacology identify the anti-virus and anti- inflammatory activities of Shuanghuanglian oral liquid used in Chinese medicine for respiratory tract infection?

Can network pharmacology identify the anti-virus and anti- inflammatory activities of Shuanghuanglian oral liquid used in Chinese medicine for respiratory tract infection?

Molecular Mechanisms Underlying Protective Role of Quercetin on Copper Sulfate-Induced Nephrotoxicity in Mice

HSA-MIR-203/MyD88 axis mediates the protective effect of hispidulin on LPS-induced apoptosis in a human renal tubular epithelial line, HK-2

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