Rhizobiumhost-range extension by introduction ofRhizobium trifolii nodGenes

Nodulation ofRhizobium frediiUSDA192 containingRhizobium leguminosarumbv.trifoliiANU843nodgenes on homologous host soybean and heterologous host clover

“…trifolii, the transconjugants induced nodules only on alfalfa, the host of R. meliloti (Hirsch et al 1985;Debelle et al 1988). In other cases, the nodules induced by nod genes transconjugants did not fix nitrogen (Johnston et al 1978;Kondorosi et al 1982;Akao et al 1994). However when the Sym plasmid was transferred to the Sym-cured strains, the transconjugants formed effective nodules depending on the donor (Beynon et al 1980;Appelbaum et al 1985).…”