<i>RhNAC2</i> and <i>RhEXPA4</i> Are Involved in the Regulation of Dehydration Tolerance during the Expansion of Rose Petals

Dai, Fanwei; Zhang, Changqing; Jiang, Xinqiang; Mei, Kang; Yin, Xiaoxing; Lü, Peitao; Zhang, Xiao; Zheng, Yi; Gao, Junping

doi:10.1104/pp.112.207720

Cited by 117 publications

(119 citation statements)

References 114 publications

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“…There is increasing evidence demonstrating that expansins are involved in the responsive mechanisms of plant species to water stress (Dai et al, 2012;Zhao et al, 2012;Barcia et al, 2014). Recently, Zhou et al (2015) have suggested that water stress inhibits leaf growth; however, changes in expansin activity and cell wall susceptibility to expansin during times of water deficit could increase the growth rate after rehydration.…”

Section: Discussionmentioning

confidence: 99%

Gene expression changes in response to drought stress in Ilex paraguariensis leaves

Acevedo¹,

Ruíz²,

Sansberro³

2016

Plant Omics

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Ilex paraguariensis St. Hil. is recognized as a shade-tolerant tree, cultivated in north-eastern Argentina, south-eastern Brazil, and Paraguay. Its leaves and young shoots are industrialised and; hence, being used to prepare a traditional tea-like infusion (mate-tea) with various medicinal benefits. Under the prevailing agro-climatic conditions in NE Argentina and with the occurrence of high temperatures and scarce rains during spring or summer, I. paraguariensis plants are subjected to water stress, reducing their yield. Differential Display was used to compare differences in gene expression between stressed and unstressed (control) potted plants. We found 52 differentially expressed transcripts (DETs), analysing banding patterns of Differential Display polyacrylamide gels, in which 38 DETs were induced, and 14 DETs were repressed in response to drought. Sequence comparisons revealed that 19% of the fragments showed no homologies, whereas 40.5 % of the total number of fragments had strong similarities with database entries of known functions. The other DETs belonged to unclassified proteins or genes with unknown functions. Interestingly, 12% of the DNA sequences were related to signal transduction, and 2% of the sequences were identified as transcription factors. Approximately, 12% of the annotated sequences were involved in stress tolerance and metal homeostasis, while 7% of the DETs were associated with photosynthesis. Other groups, representing 12%, included those involved in secondary metabolism, lignin biosynthesis, cell walls and RNA stabilization/chaperone activity. When integrated with physiological data, our results suggest that the transcriptional activity of some of the studied genes could be related to the degree of drought tolerance/susceptibility of I. paraguariensis.

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Section: Discussionmentioning

confidence: 99%

Gene expression changes in response to drought stress in Ilex paraguariensis leaves

Acevedo¹,

Ruíz²,

Sansberro³

2016

Plant Omics

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“…The EMSA was performed as described previously (Dai et al, 2012). Expression of the GST-RhHB6 fusion protein was induced in 100-mL cultures of transformed E. coli BL21 cells (see above) by addition of isopropylthio-b-galactoside to a final concentration of 0.2 mM, before the cultures were incubated at 20°C for 6 h. The recombinant proteins were extracted from the cells and purified using glutathione Sepharose 4B beads according to the manufacturer's instructions (GE Healthcare).…”

Section: Purification Of Gst-rhhb6 Recombinant Protein and Emsamentioning

confidence: 99%

“…Silencing of RhPR10.1 and RhHB6 in petal discs and plantlets by VIGS was conducted as described previously Dai et al, 2012;Tian et al, 2014), but with some modifications. The pTRV1, pTRV2, pTRV-RhPR10.1, and pTRV-RhHB6 vectors were transformed into Agrobacterium tumefaciens strain GV3101.…”

Section: Vigsmentioning

confidence: 99%

An Ethylene-Induced Regulatory Module Delays Flower Senescence by Regulating Cytokinin Content

Jia

et al. 2016

Plant Physiol.

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103

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ORCID IDs: 0000-0002-6288-7917 (Y.J.); 0000-0002-5972-7963 (C.-Z.J.); 0000-0002-3866-0894 (C.M.); 0000-0002-9285-2539 (J.G.).In many plant species, including rose (Rosa hybrida), flower senescence is promoted by the gaseous hormone ethylene and inhibited by the cytokinin (CTK) class of hormones. However, the molecular mechanisms underlying these antagonistic effects are not well understood. In this study, we characterized the association between a pathogenesis-related PR-10 family gene from rose (RhPR10.1) and the hormonal regulation of flower senescence. Quantitative reverse transcription PCR analysis showed that RhPR10.1 was expressed at high levels during senescence in different floral organs, including petal, sepal, receptacle, stamen, and pistil, and that expression was induced by ethylene treatment. Silencing of RhPR10.1 expression in rose plants by virusinduced gene silencing accelerated flower senescence, which was accompanied by a higher ion leakage rate in the petals, as well as increased expression of the senescence marker gene RhSAG12. CTK content and the expression of three CTK signaling pathway genes were reduced in RhPR10.1-silenced plants, and the accelerated rate of petal senescence that was apparent in the RhPR10.1-silenced plants was restored to normal levels by CTK treatment. Finally, RhHB6, a homeodomain-Leu zipper I transcription factor, was observed to bind to the RhPR10.1 promoter, and silencing of its expression also promoted flower senescence. Our results reveal an ethylene-induced RhHB6-RhPR10.1 regulatory module that functions as a brake of ethylenepromoted senescence through increasing the CTK content.

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“…Similarly, genes encoding multiple NAC TFs such as NAC062, NAC052, NAC2, and NAC1 were upregulated in the super ovary (Fig. 9C), including the homolog of RhNAC2 (Csa3G852460) that regulates cell expansion through binding to the promoter of RhEXPA4 in rose petals and Arabidopsis (Dai et al, 2012). These data suggested that the enlarged petal size in the super ovary may be caused by increased cell proliferation and cell expansion mediating by TCP and NAC TFs.…”

Section: Transcriptomic Differentiation Of the Normal And Super Ovarimentioning

confidence: 69%

“…Cell wall extensibility is believed to be a limiting factor for petal expansion (Yamada et al, 2009b). The expressions of genes related to cell wall function such as those that encode xyloglucan endotransglucosylase/hydrolase and expansins are positively correlated with the petal growth during flower opening (Harada et al, 2011;Dai et al, 2012;Shinozaki et al, 2014). Hormones, including ethylene, gibberellins, and auxin, regulate petal growth and flower opening.…”

mentioning

confidence: 99%

Integration of Hormonal and Nutritional Cues Orchestrates Progressive Corolla Opening

Sun

Zhao

et al. 2016

Plant Physiology

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Flower opening is essential for pollination and thus successful sexual reproduction; however, the underlying mechanisms of its timing control remain largely elusive. We identify a unique cucumber (Cucumis sativus) line '6457' that produces normal ovaries when nutrients are under-supplied, and super ovaries (87%) with delayed corolla opening when nutrients are oversupplied. Corolla opening in both normal and super ovaries is divided into four distinct phases, namely the green bud, green-yellow bud, yellow bud, and flowering stages, along with progressive color transition, cytological tuning, and differential expression of 14,282 genes. In the super ovary, cell division and cell expansion persisted for a significantly longer period of time; the expressions of genes related to photosynthesis, protein degradation, and signaling kinases were dramatically upregulated, whereas the activities of most transcription factors and stress-related genes were significantly down-regulated; concentrations of cytokinins (CKs) and gibberellins were higher in accordance with reduced cytokinin conjugation and degradation and increased expression of gibberellin biosynthesis genes. Exogenous CK application was sufficient for the genesis of super ovaries, suggesting a decisive role of CKs in controlling the timing of corolla opening. Furthermore, 194 out of 11,127 differentially expressed genes identified in pairwise comparisons, including critical developmental, signaling, and cytological regulators, contained all three types of cis-elements for CK, nitrate, and phosphorus responses in their promoter regions, indicating that the integration of hormone modulation and nutritional regulation orchestrated the precise control of corolla opening in cucumber. Our findings provide a valuable framework for dissecting the regulatory pathways for flower opening in plants.The flower is the most distinguishing organ in higher plants, and artists and scientists have been attracted to explore the mystery of flower structure and origin for decades. The evolution of flowering plants was greatly accelerated approximately 100 million years ago with the development of flowers, which were essential for recruiting animals to help distribute pollen and seeds (Danielson and Frommer, 2013). Flowers are produced from a specialized structure in the shoot tip called the shoot apical meristem, which consists of a pool of stem cells that continuously divide and replenish themselves (Fletcher et al., 1999). Morphologically, flowers are comprised of four basic structures arranged in concentric whorls: sepals in the outermost whorl 1, petals in whorl 2, stamens in whorl 3, and carpels in the innermost whorl 4. Bisexual flowers possess all four basic structures, while unisexual flowers lack one or more structures, usually the male organ stamen or the female organ carpel (Dellaporta and Calderon-Urrea, 1993). In flowering plants, approximately 90% species produce bisexual flowers, 6% species are dioecious with male and female flowers on separate plants, and 4% species a...

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RhNAC2 and RhEXPA4 Are Involved in the Regulation of Dehydration Tolerance during the Expansion of Rose Petals

Cited by 117 publications

References 114 publications

Gene expression changes in response to drought stress in Ilex paraguariensis leaves

Gene expression changes in response to drought stress in Ilex paraguariensis leaves

An Ethylene-Induced Regulatory Module Delays Flower Senescence by Regulating Cytokinin Content

Integration of Hormonal and Nutritional Cues Orchestrates Progressive Corolla Opening

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