<i>Rickettsia parkeri</i>in<i>Dermacentor parumapertus</i>Ticks, Mexico

Sánchez‐Montes, Sokani; López‐Pérez, Andrés M.; Guzmán-Cornejo, Carmen; Colunga‐Salas, Pablo; Becker, Ingeborg; Mora, Jesús Delgado-de la; Licona-Enríquez, Jesús David; Mora, David Delgado-de la; Karpathy, Sandor E.; Paddock, Christopher D.; Suzán, Gerardo

doi:10.3201/eid2406.180058

Cited by 32 publications

(25 citation statements)

References 13 publications

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“…To extract DNA, we used the Cheelex-100 protocol as previously reported ( 7 , 8 ). To evaluate the DNA quality of samples, we amplified a 400-bp segment of the ixodid 16S rRNA gene ( 5 ).…”

mentioning

confidence: 99%

“…We screened DNA extracts for Rickettsia species using a PCR targeting an 800-bp segment of the citrate synthase ( gltA ) gene. With gltA -positive samples, we performed PCRs amplifying segments of the htrA (549-bp), sca0 (532-bp), and sca5 (862-bp) genes ( 7 , 8 ). We purified PCR products using Agencourt AMPure XP (https://www.beckman.com) and sequenced amplicons on the ABI 3730xL DNA Analyzer (https://www.thermofisher.com) at the Sequencing Unit of the National Institute of Genomic Medicine (Mexico City, Mexico).…”

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confidence: 99%

“…parkeri lineages have been detected circulating in Mexico: R . parkeri strain black gap in the rabbit tick ( Dermacentor parumapertus ) in Sonora and Chihuahua ( 7 ) and R . parkeri sensu stricto associated with A .…”

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confidence: 99%

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Molecular Confirmation of Rickettsia parkeri in Amblyomma ovale Ticks, Veracruz, Mexico

Sánchez‐Montes¹,

Ballados‐González²,

Hernández-Velasco³

et al. 2019

Emerg. Infect. Dis.

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“…To extract DNA, we used the Cheelex-100 protocol as previously reported ( 7 , 8 ). To evaluate the DNA quality of samples, we amplified a 400-bp segment of the ixodid 16S rRNA gene ( 5 ).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular Confirmation of Rickettsia parkeri in Amblyomma ovale Ticks, Veracruz, Mexico

Sánchez‐Montes¹,

Ballados‐González²,

Hernández-Velasco³

et al. 2019

Emerg. Infect. Dis.

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“…parkeri has also been described as a causative agent of human rickettsiosis in other countries like Argentina [49], and Brazil [50] with Amblyommatriste ticks haven been implicated as vectors for this infection [49]. R. parkeri has equally been described as an emerging zoonotic pathogen in Mexico [51]. Similarly, [52] reported a case of R. parkeri infection from a Spanish traveller returning from Uruguay, who was con rmed bitten previously by Amblyomma triste tick.…”

Section: Discussionmentioning

confidence: 99%

Evidence of pathogenic zoonotic tick-borne Rickettsia and Borrelia spp. in some communal farms in the Eastern Cape Province, South Africa

Adelabu

Iweriebor

Okoh

et al. 2020

Preprint

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Abstract The abundance of tick populations in South Africa represents a probable risk for both animal and human health. Rickettsia spp. and Borrelia spp. are well-known significant agents of emerging human tick-borne infectious diseases throughout the world. Nevertheless, the epidemiology of their infections has been underreported in South Africa, therefore, the aim of this study was to profile for zoonotic Rickettsia and Borrelia species from ticks infesting domesticated animals in the Eastern Cape, South Africa. Morphological and molecular identification techniques were conducted on 1,200 tick samples collected from domestic animals before screening for the target bacterial pathogens. The molecular identification of the tick samples was based on the amplification of the 12S rRNA mitochondrial DNA while those of Rickettsia and Borrelia species were carried out by amplifying fragments of gltA, ompA and ompB genes for Rickettsia and flaB gene for Borrelia spp. Thereafter, the positive ticks, Borrelia and Rickettsia ompB amplicons were sequenced and further analysed. Eight species of ticks belonging to three genera; Rhipicephalus, Amblyomma, and Haemaphysalis were identified. A total of 320 (27%) samples were confirmed positive for Rickettsia out of which 74 (23%) were positive for both ompA and ompB genes. Phylogenetic analysis of ompB revealed a high homology to rickettsial reference strains from GenBank, while there was no positive result for Borrelia. The generated sequences showed 99.1 to 100% homology with R. africae-KX227790 (100 %), R. parkeri - KY113111 (99.8 %), R. peacock (99.3 %) and R. slovaca - KJ675445, JX683122 (99.1 %) representative sequences in GenBank. The findings from this study revealed that ticks collected from domesticated animals were parasitized by Rickettsia species with possible zoonotic potential, which is detrimental to human health if bitten by infected ticks.

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“…First, the interpretation of canine serologic assays can be difficult because there is cross‐reactivity between different SFGR and the aggregate immune response measured by such assays are cumulative over time. Rickettsial agents, such as R. rhipicephali, R. parkeri, R. rickettsii and R. massiliae , have been identified in the border region (Eremeeva et al, ; Herrick et al, ; Sanchez‐Montes et al, ) and may account for an undetermined portion of the SFGR antibodies present in dogs. Second, the dogs sampled in this study were a convenience sample and may not be representative of the canine population in Mexico.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of spotted fever group rickettsiae in canines along the United States–Mexico border

Pieracci

Rosa²,

Rubio³

et al. 2019

Zoonoses and Public Health

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Portions of northern Mexico are experiencing a re‐emergence of Rocky Mountain spotted fever (RMSF), a tickborne disease caused by Rickettsia rickettsii, a member of the spotted fever group of rickettsiae (SFGR). Infection with R. rickettsii can result in serious and life‐threatening illness in people and dogs. Canine seroprevalence has been used as a sentinel for human RMSF in previous studies. This study aims to quantify SFGR seroprevalence in canines in three northern Mexican states and identify risk factors associated with seropositivity. A total of 1,136 serum samples and 942 ticks were obtained from dogs participating in government sterilization campaigns and from animal control facilities in 14 Mexican cities in three states. SFGR antibodies were detected using indirect immunofluorescence antibody assays at titre values ≥1/64. Six per cent (69 dogs) showed antibodies to SFGR, with the highest seroprevalence reported in Baja California (12%), Coahuila (4%) and Sonora (4%). Dogs from Baja California had three times higher odds of having SFGR antibodies compared to dogs from Sonora (OR = 3.38, 95% CI, 1.81–6.37). Roughly one quarter (25%) of surveyed dogs were parasitized by ticks (Rhipicephalus sanguineus sensu lato) at the time of sample collection. A portion of collected ticks were tested for rickettsial DNA using polymerase chain reaction. Positive samples were then sequenced, showing evidence of SFGR including R. massiliae, R. parkeri and R. rickettsii. Dogs that spent the majority of time on the street, such as free‐roaming or community‐owned dogs, showed a greater risk of tick infestation, seropositivity, bearing seropositive ticks, and may play a pivotal role in the spread of SFGR among communities. Estimating the seroprevalence of SFGR in the canine population can help public health campaigns target high‐risk communities for interventions to reduce human RMSF cases.

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Rickettsia parkeriinDermacentor parumapertusTicks, Mexico

Cited by 32 publications

References 13 publications

Molecular Confirmation of Rickettsia parkeri in Amblyomma ovale Ticks, Veracruz, Mexico

Molecular Confirmation of Rickettsia parkeri in Amblyomma ovale Ticks, Veracruz, Mexico

Evidence of pathogenic zoonotic tick-borne Rickettsia and Borrelia spp. in some communal farms in the Eastern Cape Province, South Africa

Seroprevalence of spotted fever group rickettsiae in canines along the United States–Mexico border

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