Rosmarinus officinalis L.: micropropagation and callus induction for cell biomass development

Mascarello, C.; Sacco, E.; Pamato, M.; Silvestro, D. Di; Bassolino, Laura; Cervelli, C.; Ruffoni, B.

doi:10.17660/actahortic.2017.1155.92

Cited by 3 publications

(1 citation statement)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in many cases with other species of oregano and plants belonging to Lamiaceae family, MS medium has been the basal one in most researches. Studies that reflect this include the micropropagation in Origanum minutiflorum [33], O. vulgare [34], O. majorana [35], O. sipyleum [36], O. acutidens [26], O. syriacum and O. ehrenbergii [37], Lavandula angustifolia [38], Salvia rosmarinus [39],…”

Section: Discussionmentioning

confidence: 99%

Efficient Plant Regeneration from Explants of Compact Oregano (Origanum compactum Bentham)

Benkaddour¹,

Ali²,

Badoc³

et al. 2022

AJPS

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Efficient Plant Regeneration from Explants of Compact Oregano (Origanum compactum Bentham)

Benkaddour¹,

Ali²,

Badoc³

et al. 2022

AJPS

View full text Add to dashboard Cite

show abstract

Plant Tissue Culture: A Potential Tool for the Production of Secondary Metabolites

Garg,

Datta,

Ahmad

2024

In Vitro Propagation and Secondary Metabolite Production From Medicinal Plants: Current Trends (Part 2)

View full text Add to dashboard Cite

Plants are an immense source of phytochemicals with therapeutic effects and are widely used as life-saving drugs, and other products of varied applications. Plant tissue culture is a unique technique employed under aseptic conditions from different plant parts called explants (leaves, stems, roots, meristems, etc.) for in vitro regeneration and multiplication of plants and synthesis of secondary metabolites (SMs). Selection of elite germplasm, high-producing cell lines, strain enhancements, and optimization of media and plant growth regulators may lead to increased in vitro biosynthesis of SMs. Interventions in plant biotechnology, like the synthesis of natural and recombinant bioactive molecules of commercial importance, have attracted attention over the past few decades; and the rate of SMs biosynthesis has increased manifold than the supply of intact plants, leading to a quick acceleration in its production through novel plant cultures. Over the years, the production of SMs in vitro has been enhanced by standardising cultural conditions, selection of high-yielding varieties, application of transformation methods, precursor feeding, and various immobilization techniques; however, most often, SM production is the result of abiotic or biotic stresses, triggered by elicitor molecules like natural polysaccharides (pectin and chitosan) that are used to immobilize and cause permeabilization of plant cells. In vitro synthesis of SMs is especially promising in plant species with poor root systems, difficulty in harvesting, unavailability of elite quality planting material, poor seed set and germination, and difficult to propagate species. Thus, the present article reviews various biotechnological interventions to enhance commercially precious SMs production in vitro.

show abstract

Sustainable Exploitation of Greek Rosmarinus officinalis L. Populations for Ornamental Use through Propagation by Shoot Cuttings and In Vitro Cultures

et al. 2022

View full text Add to dashboard Cite

Rosmarinus officinalis L. belongs to the Lamiaceae family and is cultivated worldwide due to its diverse uses as an ornamental evergreen shrub in gardening, as well as a food seasoning and a natural medicine. The present research was conducted to study the morphological traits of seven wild-grown rosemary populations in Greece, as well as the propagation of two selected populations with the most desirable ornamental characteristics, by the use of shoot cuttings and in vitro cultures. From the study of the morphological traits of the seven populations, those with interesting features were grown in the areas of Amaliada and Piperia and, thus, were chosen for studying their propagation. Rooting of the shoot cuttings was carried out during the four seasons of the year, using potassium salt Indole-3-butyric acid (K-IBA) at concentrations of 0.5 and 1 g·L−1, in various substrates under the intermittent mist or fog system. It was found that the shoot cuttings rooted easily in all four seasons, but there were better results for the population of ‘Piperia’ in autumn, with 1 g·L−1 K-IBA (80%), and ‘Amaliada’ in spring, with 0.5 g·L−1 K-IBA (82.5%), while higher rooting percentages were achieved in the fog system, on a substrate consisting of perlite and peat, in a ratio of 2:1 (85%). For the in vitro cultures, shoot tips excised from the two selected populations were successfully disinfested by pre-soaking in an antioxidant solution and then, by sterilizing them in 0.6% (w/v) NaOCl, followed by transferring them onto a Murashige and Skoog (MS) nutrient medium. ‘Amaliada’ cultures proved to be the most productive population (2.1 shoots per explant), with the highest shoot formation frequency (91.6%), when cultivated on the MS nutrient medium without plant growth regulators. For ‘Piperia’ cultures, the highest shoot formation frequency (66.6%) was achieved on the MS nutrient medium supplemented with 0.25 or 0.5 mg·L−1 6-Benzyloaminopurine (BAP) and 0.1 mg·L−1 Indole-3-acetic acid (IAA). Spontaneous root formation frequency was noticed on the MS nutrient medium, containing 0.5 mg·L−1 BAP and 0.1 mg·L−1 IAA, for both ‘Amaliada’ and ‘Piperia’ cultures (50% and 41.6%, respectively) in a single stage, with root lengths of 7.1 and 5.3 cm, respectively. Rosemary plantlets, with roots formed in vitro after transplanting them in soilless substrate, were acclimatized adequately in the greenhouse environment (~70%).

show abstract

Rosmarinus officinalis L.: micropropagation and callus induction for cell biomass development

Cited by 3 publications

References 2 publications

Efficient Plant Regeneration from Explants of Compact Oregano (<i>Origanum compactum</i> Bentham)

Efficient Plant Regeneration from Explants of Compact Oregano (<i>Origanum compactum</i> Bentham)

Plant Tissue Culture: A Potential Tool for the Production of Secondary Metabolites

Sustainable Exploitation of Greek Rosmarinus officinalis L. Populations for Ornamental Use through Propagation by Shoot Cuttings and In Vitro Cultures

Contact Info

Product

Resources

About

Rosmarinus officinalis L.: micropropagation and callus induction for cell biomass development

Cited by 3 publications

References 2 publications

Efficient Plant Regeneration from Explants of Compact Oregano (&lt;i&gt;Origanum compactum&lt;/i&gt; Bentham)

Efficient Plant Regeneration from Explants of Compact Oregano (&lt;i&gt;Origanum compactum&lt;/i&gt; Bentham)

Plant Tissue Culture: A Potential Tool for the Production of Secondary Metabolites

Sustainable Exploitation of Greek Rosmarinus officinalis L. Populations for Ornamental Use through Propagation by Shoot Cuttings and In Vitro Cultures

Contact Info

Product

Resources

About

Efficient Plant Regeneration from Explants of Compact Oregano (<i>Origanum compactum</i> Bentham)

Efficient Plant Regeneration from Explants of Compact Oregano (<i>Origanum compactum</i> Bentham)