<i>rpoB</i> gene mutations in rifampin-resistant <i>Mycobacterium tuberculosis</i> isolates from rural areas of Zhejiang, China

Zeng, Meichun; Jia, Qingjun; Tang, Lei-Ming

doi:10.1177/0300060521997596

Cited by 6 publications

(1 citation statement)

References 26 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pbp1a, pbp2x, and pbp2b gene of BH16-24 is located on an inverted fragment, and its abnormal expression changes the amount of PBP protein, which may lead to BH16-24 resistance to β-lactam antibiotics. In addition, the present of the RNA polymerase beta subunit ( rpoB ) with mutations have been reported in rifamycin resistance in Brucella melitensis , Mycobacterium tuberculosis , and other microorganisms [ 36 , 37 ]. The DNA gyrase ( gyrA ) have been suggested to be involved in fluoroquinolone resistant isolates of S.agalatiae [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative genomics analysis of Streptococcus iniae isolated from Trachinotus ovatus: novel insight into antimicrobial resistance and virulence differentiation

Xiong,

Chen,

Lai

2023

BMC Genomics

View full text Add to dashboard Cite

Background Streptococcus iniae is an important fish pathogen that cause significant economic losses to the global aquaculture industry every year. Although there have some reports on the genotype of S.iniae and its relationship with virulence, no genome-scale comparative analysis has been performed so far. In our previous work, we characterized 17 isolates of S.iniae from Trachinotus ovatus and divided them into two genotypes using RAPD and rep-PCR methods. Among them, BH15-2 was classified as designated genotype A (in RAPD) and genotype 1 (in rep-PCR), while BH16-24 was classified as genotype B and genotype 2. Herein, we compared the differences in growth, drug resistance, virulence, and genome between BH15-2 and BH16-24. Results The results showed that the growth ability of BH16-24 was significantly faster than that of BH15-2 at the exponential stage. Antimicrobial tests revealed that BH15-2 was susceptible to most of the tested antibiotics except neomycin and gentamycin. In contrast, BH16-24 was resistant to 7 antibiotics including penicillin, sulfasomizole, compound sulfamethoxazole tablets, polymyxin B, spectinomycin, rifampin and ceftazidime. Intraperitoneal challenge of T.ovatus, showed that the LD50 value of BH15-2 was 4.0 × 102 CFU/g, while that of BH16-24 was 1.2 × 105 CFU/g. The genome of S.iniae BH15-2 was 2,175,659 bp with a GC content of 36.80%. Meanwhile, the genome of BH16-24 was 2,153,918 bp with a GC content of 36.83%. Comparative genome analysis indicated that compared with BH15-2, BH16-24 genome had a large-scale genomic inversion fragment, at the location from 502,513 bp to 1,788,813 bp, resulting in many of virulence and resistance genes differentially expression. In addition, there was a 46 kb length, intact phage sequence in BH15-2 genome, which was absent in BH16-24. Conclusion Comparative genomic studies of BH15-2 and BH16-24 showed that the main difference is a 1.28 Mbp inversion fragment. The inversion fragment may lead to abnormal expression of drug resistant and virulence genes, which is believed to be the main reason for the multiple resistance and weakened virulence of BH16-24. Our study revealed the potential mechanisms in underlying the differences of multidrug resistance and virulence among different genotypes of S.iniae.

show abstract

Section: Discussionmentioning

confidence: 99%