<i>Rubia tinctorum</i> root extracts: chemical profile and management of type II diabetes mellitus

Eltamany, Enas E.; Nafie, Mohamed S.; Khodeer, Dina M.; El-Tanahy, Aya H. H.; Abdel‐Kader, Maged S.; Badr, Jihan M.; Abdelhameed, Reda F. A.

doi:10.1039/d0ra03442h

Cited by 15 publications

(13 citation statements)

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“…As previously described [ 26 ], the mobile phase working solution consisted of DI-Water: Acetonitrile: Methanol in a ratio of 50:25:25. Fifty mg of weighted dry methanolic extract was dissolved in one ml of MP-WS then vortex for 2 min.…”

Section: Methodsmentioning

confidence: 99%

Chemical Profiling, Antioxidant, Cytotoxic Activities and Molecular Docking Simulation of Carrichtera annua DC. (Cruciferae)

et al. 2020

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Our investigation intended to analyze the chemical composition and the antioxidant activity of Carrichtera annua and to evaluate the antiproliferative effect of C. annua crude and phenolics extracts by MTT assay on a panel of cancerous and non-cancerous breast and liver cell lines. The total flavonoid and phenolic contents of C. annua were 47.3 ± 17.9 mg RE/g and 83.8 ± 5.3 mg respectively. C. annua extract exhibited remarkable antioxidant capacity (50.92 ± 5.64 mg GAE/g) in comparison with BHT (74.86 ± 3.92 mg GAE/g). Moreover, the extract exhibited promising reduction ability (1.17 mMol Fe+2/g) in comparison to the positive control (ascorbic acid with 2.75 ± 0.91) and it displayed some definite radical scavenging effect on DPPH (IC50 values of 211.9 ± 3.7 µg/mL). Chemical profiling of C. annua extract was achieved by LC-ESI-TOF-MS/MS analysis. Forty-nine hits mainly polyphenols were detected. Flavonoid fraction of C. annua was more active than the crude extract. It demonstrated selective cytotoxicity against the MCF-7 and HepG2 cells (IC50 = 13.04 and 19.3 µg/mL respectively), induced cell cycle arrest at pre-G1 and G2/M-phases and displayed apoptotic effect. Molecular docking studies supported our findings and revealed that kaempferol-3,7-O-bis-α-L-rhamnoside and kaempferol-3-rutinoside were the most active inhibitors of Bcl-2. Therefore, C. annua herb seems to be a promising candidate to further advance anticancer research. In extrapolation, the intake of C. annua phenolics might be adventitious for alleviating breast and liver malignancies and tumoral proliferation in humans.

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Section: Methodsmentioning

confidence: 99%

Chemical Profiling, Antioxidant, Cytotoxic Activities and Molecular Docking Simulation of Carrichtera annua DC. (Cruciferae)

et al. 2020

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“…Although the geographical location, time of year and age of the plant are known to influence the composition [ 15 ], the observed differences should be mainly ascribed to differences in both the extractive chemicals and in the extraction process (nature of the alcoholic solvent, alcohol:water ratio and mechanical enhancers such as sonication [ 36 , 37 ]), and to the characterization technique, provided that previous studies on R. tinctorum extracts [ 36 , 38 , 39 , 40 , 41 ] were conducted by HPLC and LC–HRMS (instead of GC–MS) and generally focused only on anthraquinones, anthraquinone glycosides and aglycones.…”

Section: Discussionmentioning

confidence: 99%

Activity of Anthracenediones and Flavoring Phenols in Hydromethanolic Extracts of Rubia tinctorum against Grapevine Phytopathogenic Fungi

Langa-Lomba

Sánchez-Hernández

Buzón-Durán

et al. 2021

Plants

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In this work, the chemical composition of Rubia tinctorum root hydromethanolic extract was analyzed by GC–MS, and over 50 constituents were identified. The main phytochemicals were alizarin-related anthraquinones and flavoring phenol compounds. The antifungal activity of this extract, alone and in combination with chitosan oligomers (COS) or with stevioside, was evaluated against the pathogenic taxa Diplodia seriata, Dothiorella viticola and Neofusicoccum parvum, responsible for the so-called Botryosphaeria dieback of grapevine. In vitro mycelial growth inhibition tests showed remarkable activity for the pure extract, with EC50 and EC90 values as low as 66 and 88 μg·mL−1, respectively. Nonetheless, enhanced activity was attained upon the formation of conjugate complexes with COS or with stevioside, with synergy factors of up to 5.4 and 3.3, respectively, resulting in EC50 and EC90 values as low as 22 and 56 μg·mL−1, respectively. The conjugate with the best performance (COS–R. tinctorum extract) was then assayed ex situ on autoclaved grapevine wood against D. seriata, confirming its antifungal behavior on this plant material. Finally, the same conjugate was evaluated in greenhouse assays on grafted grapevine plants artificially inoculated with the three aforementioned fungal species, resulting in a significant reduction in the infection rate in all cases. This natural antifungal compound represents a promising alternative for developing sustainable control methods against grapevine trunk diseases.

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“…Licorice was considered a panacea for ma diseases in oriental medicine. The Egyptians, Greeks, and Romans recommended licor Rubia tinctorum L. (Rubiaceae) is also a medicinal plant, according to modern studies, containing about 250 compounds with different chemical classes [123]. The main components are anthraquinones and their derivatives such as alizarin and purpurin, which have a diuretic, antispasmodic, and laxative effect [124].…”

Section: The Collection Of Hairy Roots Of the Institute Of Plant Phys...mentioning

confidence: 99%

“…The main components are anthraquinones and their derivatives such as alizarin and purpurin, which have a diuretic, antispasmodic, and laxative effect [124]. At present, the antitumor, hepatoprotective and antidiabetic properties of the roots of common madder have been shown [123,125,126]. Besides that, the roots of common madder have been used as a dye for dyeing fabrics and applying patterns since ancient times [127,128].…”

Section: The Collection Of Hairy Roots Of the Institute Of Plant Phys...mentioning

confidence: 99%

Collection of Hairy Roots as a Basis for Fundamental and Applied Research

et al. 2022

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Due to population growth, instability of climatic conditions, and reduction of the areas of natural ecosystems, it becomes necessary to involve modern biotechnological approaches to obtain highly productive plant material. This statement applies both to the creation of plant varieties and the production of new pharmaceutical raw materials. Genetic transformation of valuable medicinal plants using Agrobacterium rhizogenes ensures the production of stable and rapidly growing hairy roots cultures that have a number of advantages compared with cell culture and, above all, can synthesize root-specific substances at the level of the roots of the intact plant. In this regard, special attention should be paid to the collection of hairy roots of the Institute of Plant Physiology RAS, Russian Academy of Sciences, the founder of which was Dr. Kuzovkina I.N. Currently, the collection contains 38 hairy roots lines of valuable medicinal and forage plants. The review discusses the prospects of creating a hairy roots collection as a basis for fundamental research and commercial purposes.

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Rubia tinctorum root extracts: chemical profile and management of type II diabetes mellitus

Abstract: The chemical and biological profiling of the root extracts of Rubia tinctorum was performed.

Cited by 15 publications

References 45 publications

Chemical Profiling, Antioxidant, Cytotoxic Activities and Molecular Docking Simulation of Carrichtera annua DC. (Cruciferae)

Chemical Profiling, Antioxidant, Cytotoxic Activities and Molecular Docking Simulation of Carrichtera annua DC. (Cruciferae)

Activity of Anthracenediones and Flavoring Phenols in Hydromethanolic Extracts of Rubia tinctorum against Grapevine Phytopathogenic Fungi

Collection of Hairy Roots as a Basis for Fundamental and Applied Research

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