S-Glycosylation of Fluensulfone in Tomatoes: An Important Way of Fluensulfone Metabolism

Abstract

Fluensulfone (FSF) becomes increasingly popular because of its nonfumigation application method. However, studies on the metabolic mechanism of FSF in plants are lacking. Here, tomato seedling was cultivated in hydroponic media to investigate the connection among FSF’s metabolism in tomato, the regulation of tomato endogenous glycosides, and the elimination of hydrogen peroxide in tomato cells. The accumulation of FSF was only detected in the lower stems of tomatoes; FSF was mainly metabolized into S-glycosylated conjugates in the roots, and the roots were the tissues with the highest metabolite content; and no FSF and metabolites were detected in the upper leaves. In response to FSF stress (2 mg/L for 7 d), the content of sugar and glycosides in the stems of tomato seedlings significantly increased. The amount of some compounds on the pathway related to glucose was affected by FSF. The three precursor compounds (homomethioine, isoleucine, and l-tyrosine) in the pathway of glucosinolate biosynthesis increased significantly under the stress of FSF, which indicates that FSF may compete with them for UGT74B1. Besides, FSF-induced flavonoid glycosides may play a role in the process of removing hydrogen peroxide. This research provides inspiration for the fate of many xenobiotics containing sulfonyl groups in plants.

Dissection of Paenibacillus polymyxa NSY50-Induced Defense in Cucumber Roots against Fusarium oxysporum f. sp. cucumerinum by Target Metabolite Profiling

“…The acquired MS data from GC–MS and LC–MS were analyzed with the metabolomics processing software Progenesis QI v2.3 software (Waters Corporation, Milford, CT, USA) for baseline filtering, peak identification, integration, and retention, as described by Zhang et al [ 36 ]. Briefly, the main parameters were as follows: precursor tolerance of 5 ppm, product tolerance of 10 ppm, product ion threshold of 5%, and retention time tolerance of 0.02 min.…”

Дослідження Токсикологічного Впливу Гербіциду Атразину На Проростки Люцерни

“…The alfalfa was washed and dried, and the biomass and physiological indexes of plants were measured. Among them, the content of chlorophyll was determined by acetone method, referring to the methods of Yin et al (2008) and Zhang et al (2017), the content of malondialdehyde -by the content of thionenenebb barbituric acid reactants (TBARS), referring to the method of Zhang et al (2021).…”

“…At the same time, it was also found that under atrazine stress, the fresh weight and chlorophyll content of shallot decreased to varying degrees, and the root activity and activity decreased (Wang et al, 2011). Under the treatment of atrazine at medium and high concentrations, the biomass and a series of physiological and biochemical indicators of Phyllostachys chinensis were also greatly affected (Zhang et al, 2021). The residual amount of atrazine in farmland will be toxic, and will also be toxic to subsequent crops such as rice, wheat, soybean, and sugar beet.…”

Regio- and Stereo-selective Synthesis of 1β-/3R-Aryl Thiosugar

“…硫代低聚糖 S-Galp(α1→3)Galp 还可作 为在锥形虫细胞表面被溶菌抗体识别的拟糖 [5] (图 1). 药 物和天然产物的糖基化修饰具有重要意义 [6][7] , 例如 3-硫代糖修饰的蛋白质可有效应用于细胞标记 [8][9] ; 胰高 血糖素样态 1 (GLP-1)经过硫代糖的修饰后, 其生物活 性和抗酶解性都得到了显著的提升 [10] ; 硫代糖类能用 于治疗糖尿病 [11] 和调控植物细胞代谢 [12] . 此外, 硫代糖 还作为合成低聚寡糖的重要中间体而被广泛研究 [13][14] .…”