<i>S100A4</i>Gene is Crucial for Methionine-Choline-Deficient Diet-Induced Non-Alcoholic Fatty Liver Disease in Mice

Zhang, Yin Hua; Qiang, De; Ding, Deping; Li, Juan; Chen, Lin Li; Ao, Kang Jian; Tian, You

doi:10.3349/ymj.2018.59.9.1064

Cited by 7 publications

(6 citation statements)

References 36 publications

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“…Mice fed the MCD diet had increased triglycerides in the liver [22, 46, 47] and this was also found in the present study. WT2 mice had about two-fold higher triglyceride levels than WT1 animals irrespective of the diet.…”

Section: Discussionsupporting

confidence: 89%

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Variations in hepatic lipid species of age-matched male mice fed a methionine-choline-deficient diet and housed in different animal facilities

et al. 2019

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Background: Non-alcoholic steatohepatitis (NASH) is a common disease and feeding mice a methionine-cholinedeficient (MCD) diet is a frequently used model to study its pathophysiology. Genetic and environmental factors influence NASH development and liver lipid content, which was studied herein using C57BL/6 J mice bred in two different animal facilities. Methods: Age-matched male C57BL/6 J mice bred in two different animal facilities (later on referred to as WT1 and WT2) at the University Hospital of Regensburg were fed identical MCD or control chows for 2 weeks. Hepatic gene and protein expression and lipid composition were determined. Results: NASH was associated with increased hepatic triglycerides, which were actually higher in WT1 than WT2 liver in both dietary groups. Cholesterol contributes to hepatic injury but was only elevated in WT2 NASH liver. Ceramides account for insulin resistance and cell death, and ceramide species d18:1/16:0 and d18:1/18:0 were higher in the NASH liver of both groups. Saturated sphingomyelins only declined in WT1 NASH liver. Lysophosphatidylcholine concentrations were quite normal in NASH and only one of the 12 altered phosphatidylcholine species declined in NASH liver of both groups. Very few phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol species were comparably regulated in NASH liver of both animal groups. Seven of these lipid species declined and two increased in NASH. Notably, hepatic mRNA expression of proinflammatory (F4/80, CD68, IL-6, TNF and chemerin) and profibrotic genes (TGF beta and alpha SMA) was comparable in WT1 and WT2 mice.Conclusions: Mice housed and bred in different animal facilities had comparable disease severity of NASH whereas liver lipids varied among the groups. Thus, there was no specific lipid signature for NASH in the MCD model.

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Section: Discussionsupporting

confidence: 89%

“…Upregulated and unchanged IL-6 mRNA expression in MCD-NASH liver were identified in the different mouse groups analyzed herein and was also reported in separate studies by others [46, 47]. Chemerin was found increased in NASH liver of MCD diet fed rodents [48] and was also higher in one of the present models.…”

Section: Discussionsupporting

confidence: 86%

Variations in hepatic lipid species of age-matched male mice fed a methionine-choline-deficient diet and housed in different animal facilities

et al. 2019

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“…ORO staining was performed for the determination of TG contents in liver tissues according to published literature (20). Briefly, tissue sections were fixed for 10 min with 10% ice paraformaldehyde and washed by distilled water three times after placed on slides for 30 min at room temperature.…”

Section: Oro Stainingmentioning

confidence: 99%

“…According to the earlier paper (20), liver sections were executed HE stains for measurement of hepatic histology. The tissue sections were observed and photographed with ×100 and ×200 original magnification under an electronic microscope.…”

Section: The He Stainsmentioning

confidence: 99%

Blueberry polyphenols play a preventive effect on alcoholic fatty liver disease C57BL/6 J mice by promoting autophagy to accelerate lipolysis to eliminate excessive TG accumulation in hepatocytes

Zhuge¹,

Zhang²,

Liu³

et al. 2020

Ann Palliat Med

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Background: This study aims to explore blueberry polyphenols and its roles in nonalcoholic fatty liver disease (NAFLD) by relieving hepatic steatosis, and to understand alcoholic fatty liver disease (AFLD).Cell autophagy has been proved to promote lipid metabolism and is involved in the pathogenesis of AFLD; however, whether blueberry polyphenol affects autophagy is unknown. Therefore, our study analyzes the functions of blueberry polyphenol on AFLD and if its mechanisms are engaged with hepatocytes autophagy. Methods:We built the AFLD mice model via alcohol abduction, and the TG lipid droplets content detected the hepatic steatosis through ORO and HE stains. For blood lipid levels measurements, serum CHOL and TG concentrations were tests. For mechanism analysis, the lipogenic genes of SREBP1, FAS, and ACCα, and the lipodieretic genes of ATGL and Sirt1 were evaluated by qRT-PCR, as well as the autophagy proteins of p62; WB measured LC3-I and LC3-II.Results: We found that chronic alcohol intake successfully induced AFLD occurrence with increased TG lipid droplets content in liver and serum CHOL and TG levels that accompanied by increased lipogenic and reduced lipodieretic mRNA levels, as well as enhancive p62 protein and decreased LC3-II/LC3-I proportion.However, after blueberry polyphenol intake, there were opposite outcomes happened. Moreover, blueberry polyphenol alone did not affect the lipid metabolism but promoted the hepatocytes autophagy at 200 mg/kg concentration.Conclusions: In summary, we are unparalleled that illustrated blueberry polyphenols can prevent AFLD development by promoting autophagy to accelerate lipid metabolism than to lighten hepatic steatosis.

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“…Also, we examined the correlation between AOD S100A4 and CVF in the tissue samples, and the results revealed an excellent reliable correlation between these parameters, indicating that S100A4 may represent a positive regulatory factor promoting cancer-associated collagen deposition. Studies have reported that S100A4 plays a vital role in various nontumor diseases, such as fibrosis, inflammation, immune response, and neuroprotection, where it regulates tissue fibrosis via different signaling pathways and stimulates fibroblasts to secrete collagen, inducing collagen deposition (19)(20)(21)(22)(23)(24). These results suggest that S100A4 may regulate collagen deposition in tumor disorders via other signaling pathways; we have begun to investigate the possible signaling mechanisms using in vivo and in vitro methods.…”

Section: Discussionmentioning

confidence: 97%

Quantitative shear wave elastography in primary invasive breast cancers, based on collagen-S100A4 pathology, indicates axillary lymph node metastasis

Wen¹,

Yu²,

Tian

et al. 2020

Quant Imaging Med Surg

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Background: The purpose of this study was to evaluate the value of quantitative shear wave elastography (SWE) in indicating the axillary lymph node metastasis (LNM) of invasive breast cancers (IBCs) and to investigate if S100A4 plays a key role in promoting metastasis and increasing stiffness in IBC.Methods: The differences in SWE of 223 IBC patients were compared between the LNM+ and LNMgroups and the optimal cutoff values of SWE for diagnosing LNM were calculated. We searched the gene expression omnibus (GEO) to determine whether S100A4 was more highly expressed in IBCs that were LNM+ than in those that were LNM-. Sirius red and immunohistochemical staining were used to examine the collagen deposition and S100A4 expression of included tissue samples, and correlations of SWE and S100A4 expression with collagen deposition were analyzed. Results:The optimal cutoff values for Emax (the maximum stiff value), Emean (the mean stiff value), and EmeanR (the ratio of Emean between mass and parenchyma) for diagnosing axillary LNM were 111.05 kPa, 79.80 kPa, and 6.89, respectively. GSE9893 exhibited more increased S100A4 expression in IBCs that were LNM+ than in those that were LNM-. Collagen volume fraction (CVF) and the average optical density of S100A4 (AOD S100A4 ) in the LNM+ group were significantly higher than those in the LNM-group. Emax, Emean, EmeanR, and AOD S100A4 were all positively correlated with CVF.Conclusions: SWE in primary IBC could be useful for indicating axillary LNM. S100A4 may be a factor that regulates cancer-associated collagen deposition and metastasis; however, prospective molecular biological studies are needed.

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S100A4Gene is Crucial for Methionine-Choline-Deficient Diet-Induced Non-Alcoholic Fatty Liver Disease in Mice

Cited by 7 publications

References 36 publications

Variations in hepatic lipid species of age-matched male mice fed a methionine-choline-deficient diet and housed in different animal facilities

Variations in hepatic lipid species of age-matched male mice fed a methionine-choline-deficient diet and housed in different animal facilities

Blueberry polyphenols play a preventive effect on alcoholic fatty liver disease C57BL/6 J mice by promoting autophagy to accelerate lipolysis to eliminate excessive TG accumulation in hepatocytes

Quantitative shear wave elastography in primary invasive breast cancers, based on collagen-S100A4 pathology, indicates axillary lymph node metastasis

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