Saccharomyces cerevisiae-Derived Mannan Does Not Alter Immune Responses to Aspergillus Allergens

Abstract

Severe asthma with fungal sensitization predominates in the population suffering from allergic asthma, to which there is no cure. While corticosteroids are the mainstay in current treatment, other means of controlling inflammation may be beneficial. Herein, we hypothesized that mannan from Saccharomyces cerevisiae would dampen the characteristics of fungal allergic asthma by altering the pulmonary immune responses. Using wild-type and transgenic mice expressing the human mannose receptor on smooth muscle cells, we explored the outcome of mannan administration during allergen exposure on the pathogenesis of fungal asthma through measurement of cardinal features of disease such as inflammation, goblet cell number, and airway hyperresponsiveness. Mannan treatment did not alter most hallmarks of allergic airways disease in wild-type mice. Transgenic mice treated with mannan during allergen exposure had an equivalent response to non-mannan-treated allergic mice except for a prominent granulocytic influx into airways and cytokine availability. Our studies suggest no role for mannan as an inflammatory regulator during fungal allergy.

Pustulan Activates Chicken Bone Marrow-Derived Dendritic Cells In Vitro and Promotes Ex Vivo CD4+ T Cell Recall Response to Infectious Bronchitis Virus

“…Subsequently, the cells were analysed by flow cytometry. The level of endotoxin contamination was determined by providers or previous studies to be insignificant in the glycan-based ligand concentrations used [ 34 , 37 , 38 ].…”

Gut Mycobiome and Asthma

“…Mannans were recently utilized to coat allergens, facilitating their safe and efficient uptake by immune cells for oral allergy immunotherapy [ 79 , 80 ]. However, their application in reducing the pathogenesis of Aspergillus fumigatus -induced asthma when administered intranasally has not been successful [ 81 ].…”

Initiation and Pathogenesis of Severe Asthma with Fungal Sensitization

“…Mannan derived from Saccharomyces cerevisiae , a component of the human gut mycobiome [ 161 ], can promote airway epithelial cell spreading and wound healing [ 162 ]. However, the use of mannan therapeutically did not reduce the pathogenesis of A. fumigatus -induced fungal asthma [ 163 ]. Our understanding is limited regarding the complex interplay between the gut mycobiome and development/regulation of the peripheral immune system during fungal asthma.…”