Saccharomyces cerevisiae
 var. boulardii preserves the integrity of intestinal mucosa in gilthead seabream, Sparus aurata
 subjected to a bacterial challenge with Vibrio anguillarum

Maricchiolo, Giulia; Caccamo, Letteria; Mancuso, Monique; Cusimano, Giovanni Marco; Gai, Francesco; Genovese, Maria Inês; Ghonimy, Abdallah; Genovese, Luigi

doi:10.1111/are.12862

Cited by 3 publications

(4 citation statements)

References 11 publications

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“…The histological examination results are in agreement with previous results on the same species [53], where the liver analysis showed the greatest difference between the control and the LSB300 group for all the sampling times. A significant increase in the number of hepatocytes-area was detected, thereby showing a positive effect of the probiotic on the fish, especially for the highest concentration [76].…”

Section: Discussionsupporting

confidence: 92%

“…Probiotics are effective in modulating the inflammatory response and in increasing disease resistance to bacterial infections, as demonstrated in Sander lucioperca protected by Lactobacillus brevis against Aeromonas hydrophila [81]. Moreover, Maricchiolo et al [53] demonstrated that S. cerevisiae var. boulardii, administered at a dosage of 300 mg/kg (after 21 days of feeding), is able to protect the intestinal mucosa of gilthead seabream against V. anguillarum infections.…”

Section: Discussionmentioning

confidence: 99%

“…LSB, at a dosage of 300 mg/kg, lowered the expression of the interleukin-6 gene, when it was administrated over short time periods. Indeed, Maricchiolo et al [53] recommended a diet supplementation (with this dosage) for 21 days because of its marked protective effects on intestinal mucosa. On the contrary, we observed that IL-6 was upregulated after a prolongated administration.…”

Section: Discussionmentioning

confidence: 99%

“…boulardii (S. boulardii) has a prominent place among the most studied and characterized yeasts. From a taxonomical point of view, it is a yeast that belongs to the S. cerevisiae species, and it is used worldwide for the prevention and treatment of a variety of gut inflammatory diseases in both humans and animals [46][47][48][49][50][51][52], although very few data are available on its effects on aquatic organisms [53]. The molecular basis for the beneficial effects of S. boulardii was extensively studied both in vitro and in animal models, and it was found to have an impact on inflammatory cytokine production by intestinal epithelial cells [50,54].…”

Section: Introductionmentioning

confidence: 99%

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Live Yeast (Saccharomyces cerevisiae var. boulardii) Supplementation in a European Sea Bass (Dicentrarchus labrax) Diet: Effects on the Growth and Immune Response Parameters

Perdichizzi,

Meola,

Caccamo

et al. 2023

Animals

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The present study has been aimed at evaluating the effects of the dietary inclusion of the live yeasts, Saccharomyces cerevisiae var. boulardii (LSB) administered at increasing concentrations (0, 100, and 300 mg kg−1 of feed, here referred to as LSB 0, 100, 300) for 90 days, on the health conditions of European sea bass. The main zootechnical parameters, histological and morphological analyses, innate immunity response parameters (intestinal cytokine expression, lysozyme content, spontaneous hemolytic and hemagglutinating activities, antibacterial activities, and peroxidase activity) were measured as fish welfare parameters. LSB did not impair either growth parameters or the morphometric indexes. LSB down-regulated interleukin-1β transcription in the distal gut of fish treated with 5.4 × 105 CFU g−1 (LSB100) for 21 days. The interleukin-6 mRNA level decreased significantly in the proximal gut for both doses of yeast, after 21 days of feeding; the gene expression of interleukin-6 was significantly lower in the sea bass fed 10.81 × 105 CFU g−1 (LSB300) probiotic. The levels of TNF-α mRNA were not influenced by probiotic supplementation. Increases, although not significant, in the hematological and immunological parameters were also recorded. The data collected in the present study suggests that an LSB-supplemented diet acts on the gut immune system of sea bass by modulating the expression of the key inflammatory genes.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Live Yeast (Saccharomyces cerevisiae var. boulardii) Supplementation in a European Sea Bass (Dicentrarchus labrax) Diet: Effects on the Growth and Immune Response Parameters

Perdichizzi,

Meola,

Caccamo

et al. 2023

Animals

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Functional Diets Modulate lncRNA-Coding RNAs and Gene Interactions in the Intestine of Rainbow Trout Oncorhynchus mykiss

et al. 2017

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The advent of functional genomics has sparked the interest in inferring the function of non-coding regions from the transcriptome in non-model species. However, numerous biological processes remain understudied from this perspective, including intestinal immunity in farmed fish. The aim of this study was to infer long non-coding RNA (lncRNAs) expression profiles in rainbow trout (Oncorhynchus mykiss) fed for 30 days with functional diets based on pre- and probiotics. For this, whole transcriptome sequencing was conducted through Illumina technology, and lncRNAs were mined to evaluate transcriptional activity in conjunction with known protein sequences. To detect differentially expressed transcripts, 880 novels and 9067 previously described O. mykiss lncRNAs were used. Expression levels and genome co-localization correlations with coding genes were also analyzed. Significant differences in gene expression were primarily found in the probiotic diet, which had a twofold downregulation of lncRNAs compared to other treatments. Notable differences by diet were also evidenced between the coding genes of distinct metabolic processes. In contrast, genome co-localization of lncRNAs with coding genes was similar for all diets. This study contributes novel knowledge regarding lncRNAs in fish, suggesting key roles in salmons fed with in-feed additives with the capacity to modulate the intestinal homeostasis and host health.

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Disruption of gut integrity and permeability contributes to enteritis in a fish-parasite model: a story told from serum metabolomics

et al. 2019

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Background In the animal production sector, enteritis is responsible for serious economic losses, and intestinal parasitism is a major stress factor leading to malnutrition and lowered performance and animal production efficiency. The effect of enteric parasites on the gut function of teleost fish, which represent the most ancient bony vertebrates, is far from being understood. The intestinal myxozoan parasite Enteromyxum leei dwells between gut epithelial cells and causes severe enteritis in gilthead sea bream (Sparus aurata), anorexia, cachexia, growth impairment, reduced marketability and increased mortality. Methods This study aimed to outline the gut failure in this fish-parasite model using a multifaceted approach and to find and validate non-lethal serum markers of gut barrier dysfunction. Intestinal integrity was studied in parasitized and non-parasitized fish by immunohistochemistry with specific markers for cellular adhesion (E-cadherin) and tight junctions (Tjp1 and Cldn3) and by functional studies of permeability (oral administration of FITC-dextran) and electrophysiology (Ussing chambers). Serum samples from parasitized and non-parasitized fish were analyzed using non-targeted metabolomics and some significantly altered metabolites were selected to be validated using commercial kits. Results The immunodetection of Tjp1 and Cldn3 was significantly lower in the intestine of parasitized fish, while no strong differences were found in E-cadherin. Parasitized fish showed a significant increase in paracellular uptake measured by FITC-dextran detection in serum. Electrophysiology showed a decrease in transepithelial resistance in infected animals, which showed a diarrheic profile. Serum metabolomics revealed 3702 ions, from which the differential expression of 20 identified compounds significantly separated control from infected groups in multivariate analyses. Of these compounds, serum inosine (decreased) and creatine (increased) were identified as relevant and validated with commercial kits. Conclusions The results demonstrate the disruption of tight junctions and the loss of gut barrier function, a metabolomic profile of absorption dysfunction and anorexia, which further outline the pathophysiological effects of E. leei.

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Saccharomyces cerevisiae var. boulardii preserves the integrity of intestinal mucosa in gilthead seabream, Sparus aurata subjected to a bacterial challenge with Vibrio anguillarum

Cited by 3 publications

References 11 publications

Live Yeast (Saccharomyces cerevisiae var. boulardii) Supplementation in a European Sea Bass (Dicentrarchus labrax) Diet: Effects on the Growth and Immune Response Parameters

Live Yeast (Saccharomyces cerevisiae var. boulardii) Supplementation in a European Sea Bass (Dicentrarchus labrax) Diet: Effects on the Growth and Immune Response Parameters

Functional Diets Modulate lncRNA-Coding RNAs and Gene Interactions in the Intestine of Rainbow Trout Oncorhynchus mykiss

Disruption of gut integrity and permeability contributes to enteritis in a fish-parasite model: a story told from serum metabolomics

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