<i>Saccharomyces paradoxus</i> and <i>Saccharomyces cerevisiae</i> are associated with exudates of North American oaks

Наумов, Г. И.; Наумова, Е. С.; Sniegowski, Paul D.

doi:10.1139/w98-104

Cited by 117 publications

(123 citation statements)

References 23 publications

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“…Thus, the combination of external phospholipases and Git1p provide yeast with the capability of obtaining crucial nutrients (inositol and phosphate) from environments in which PI or its deacylation product, GroPIns, are available. In a natural environment, the source of PI or GroPIns may be such things as decaying fruit or plant exudates (28). Once inside the cell, GroPIns must be metabolized to liberate inositol and phosphate (Fig.…”

Section: Deletion Of Potential Pho4p Binding Sites Results In Loss Ofmentioning

confidence: 99%

Glycerophosphoinositol, a Novel Phosphate Source Whose Transport Is Regulated by Multiple Factors in Saccharomyces cerevisiae

Almaguer

Cheng

Nolder

et al. 2004

Journal of Biological Chemistry

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Git1p mediates the transport of the phospholipid metabolite, glycerophosphoinositol, into Saccharomyces cerevisiae. We report that phosphate limitation and inositol limitation affect GIT1 expression and Git1p transport activity via distinct mechanisms that involve multiple transcription factors. GIT1 transcript levels and Git1p activity are greater in cells starved for phosphate, with or without inositol limitation, than in cells only limited for inositol. Furthermore, the kinetics of GIT1 transcript accumulation and Git1p activity upon transfer of cells to phosphate starvation media are different from those obtained upon transfer of cells to inositolfree media. Pho2p and Pho4p are required for GIT1 expression and for Git1p transport activity under all growth conditions tested. In contrast, Ino2p and Ino4p are required for full GIT1 expression when inositol is limiting, with or without phosphate limitation, but not when only phosphate is limiting. Greatly reduced transport activity was detected in ino2⌬ and ino4⌬ cells under all growth conditions. A 300-base pair region of the GIT1 promoter containing potential Pho4p binding sites was shown to be required for full GIT1 expression. Git1p appears to act as a H ؉ -symporter, and neither inositol nor phosphate effectively compete with glycerophosphoinositol for transport by Git1p. Glycerophosphoinositol was shown previously to support the growth of an inositol auxotroph. Remarkably, we now report that glycerophosphoinositol can act as the sole source of phosphate for the cell, providing functional relevance for the regulation of Git1p transport activity by phosphate.

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Section: Deletion Of Potential Pho4p Binding Sites Results In Loss Ofmentioning

confidence: 99%

Glycerophosphoinositol, a Novel Phosphate Source Whose Transport Is Regulated by Multiple Factors in Saccharomyces cerevisiae

Almaguer

Cheng

Nolder

et al. 2004

Journal of Biological Chemistry

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“…Despite our rich understanding of the cell biology of S. cerevisiae, there is relatively little information on its ecology. S. cerevisiae has been cultured from a variety of plants, such as grapes and oak tree exudates (Naumov et al 1998;Mortimer and Polsinelli 1999). In these environments it presumably must interact with a variety of insects.…”

Section: Functions Of the Spore: Dispersal To New Environmentsmentioning

confidence: 99%

Sporulation in the Budding Yeast Saccharomyces cerevisiae

2011

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In response to nitrogen starvation in the presence of a poor carbon source, diploid cells of the yeast Saccharomyces cerevisiae undergo meiosis and package the haploid nuclei produced in meiosis into spores. The formation of spores requires an unusual cell division event in which daughter cells are formed within the cytoplasm of the mother cell. This process involves the de novo generation of two different cellular structures: novel membrane compartments within the cell cytoplasm that give rise to the spore plasma membrane and an extensive spore wall that protects the spore from environmental insults. This article summarizes what is known about the molecular mechanisms controlling spore assembly with particular attention to how constitutive cellular functions are modified to create novel behaviors during this developmental process. Key regulatory points on the sporulation pathway are also discussed as well as the possible role of sporulation in the natural ecology of S. cerevisiae. TABLE OF CONTENTS Abstract 737Introduction 738

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“…Polsinelli et al reported that, before maturation, grapes are almost free of S. cerevisiae (∼0.05%), whereas 25% of ripe damaged grapes harbor such cells (6,7). Following this pioneering study, S. cerevisiae strains have been isolated from several natural sources (7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22). However, these reports are restricted to warm seasons, when ripe fruit is available, or to post harvest.…”

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confidence: 99%

Role of social wasps in Saccharomyces cerevisiae ecology and evolution

Stefanini

Dapporto

Legras

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

267

236

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Saccharomyces cerevisiae is one of the most important model organisms and has been a valuable asset to human civilization. However, despite its extensive use in the last 9,000 y, the existence of a seasonal cycle outside human-made environments has not yet been described. We demonstrate the role of social wasps as vector and natural reservoir of S. cerevisiae during all seasons. We provide experimental evidence that queens of social wasps overwintering as adults (Vespa crabro and Polistes spp.) can harbor yeast cells from autumn to spring and transmit them to their progeny. This result is mirrored by field surveys of the genetic variability of natural strains of yeast. Microsatellites and sequences of a selected set of loci able to recapitulate the yeast strain's evolutionary history were used to compare 17 environmental wasp isolates with a collection of strains from grapes from the same region and more than 230 strains representing worldwide yeast variation. The wasp isolates fall into subclusters representing the overall ecological and industrial yeast diversity of their geographic origin. Our findings indicate that wasps are a key environmental niche for the evolution of natural S. cerevisiae populations, the dispersion of yeast cells in the environment, and the maintenance of their diversity. The close relatedness of several wasp isolates with grape and wine isolates reflects the crucial role of human activities on yeast population structure, through clonal expansion and selection of specific strains during the biotransformation of fermented foods, followed by dispersal mediated by insects and other animals. evolutionary biology | genomics

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Saccharomyces paradoxus and Saccharomyces cerevisiae are associated with exudates of North American oaks

Cited by 117 publications

References 23 publications

Glycerophosphoinositol, a Novel Phosphate Source Whose Transport Is Regulated by Multiple Factors in Saccharomyces cerevisiae

Glycerophosphoinositol, a Novel Phosphate Source Whose Transport Is Regulated by Multiple Factors in Saccharomyces cerevisiae

Sporulation in the Budding Yeast Saccharomyces cerevisiae

Role of social wasps in Saccharomyces cerevisiae ecology and evolution

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