Salicornia L. (Salicornia pusilla J. Woods, S. ramosissima J. Woods, S. europaea L., S. obscura P.W. Ball &amp; Tutin, S. nitens P.W. Ball &amp; Tutin, S. fragilis P.W. Ball &amp; Tutin and S. dolichostachya Moss)

Davy, A. J.; Bishop, G. F.; Costa, César Serra Bonifácio

doi:10.1046/j.0022-0477.2001.00607.x

Cited by 184 publications

(93 citation statements)

References 143 publications

(226 reference statements)

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“…The root of halophyte S. europaea can tolerate exceptionally low water potentials, and exhibits anomalous secondary thickening with concentric series of collateral vascular bundles embedded in a lignified ground tissue [25]. After being treated with 800 mM NaCl for three weeks, its roots were significantly lignified, which made them more recalcitrant for protein isolation, although less salt ions were accumulated in roots than in shoots for salt treated halophyte S. europaea (Table 3).…”

Section: The Bpp Protocol Produces More Protein Spotsmentioning

confidence: 94%

“…The euhalophyte S. europaea accumulates large amounts of salt ions in its succulent shoots [25,29]. Using the flame emission method, we detected Na 1 contents at 75.73, 199.59 and 295.19 mg/g dry weight (DW) in its shoots treated with 0, 200 and 800 mM NaCl, respectively (Table 3).…”

Section: The Bpp Methods Efficiently Removes Salt Ions From S Europaementioning

confidence: 99%

“…The application of 2-DE for halophytes is still limited largely due to the absence of an efficient protein extraction method. This is because the halophyte cells contain high concentration of salt ions [25], which can interfere with the IEF process of protein samples over IPGs [1,4,26,27].…”

Section: Introductionmentioning

confidence: 99%

“…Salicornia europaea L. is a succulent and leafless euhalophyte that belongs to Chenopodiaceae [25,28]. It is one of the most salt-tolerant plant species in the world, and requires adequate concentration of NaCl for its optimal growth and development [25,28,29].…”

Section: Introductionmentioning

confidence: 99%

“…It is one of the most salt-tolerant plant species in the world, and requires adequate concentration of NaCl for its optimal growth and development [25,28,29]. It was reported that S. europaea could maintain low water potential in its tissues by accumulating high concentrations of inorganic ions, mainly sodium and chloride, and predominantly in the cell vacuoles [25,28]. In order to investigate the proteins responsible for salt tolerance in this species using proteomic approaches, it is necessary to establish a protocol to isolate high-quality proteins with good yields, which would also allow us to remove ions during protein preparation.…”

Section: Introductionmentioning

confidence: 99%

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A protein extraction method compatible with proteomic analysis for the euhalophyte Salicornia europaea

et al. 2007

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Protein extraction from plants like the halophyte Salicornia europaea has been problematic using standard protocols due to high concentrations of salt ions in their cells. We have developed an improved method for protein extraction from S. europaea, which allowed us to remove interfering compounds and salt ions by including the chemicals borax, polyvinylpolypyrrolidone, and phenol. The comparative study of this method with several other protocols using NaCl-treated S. europaea shoots demonstrated that this method gave the best distinction of proteins on 2-DE gels. This protocol had a wide range of applications as high yields and good distinction of 1-DE gels for proteins isolated from twelve other plants were rendered. In addition, we reported results of 2-DE using the recalcitrant tissue of the S. europaea roots. We also demonstrated that this protocol is compatible with proteomic analysis as eight specific proteins generated by this method have been identified by MS. In conclusion, our newly developed protein extraction protocol is expected to have excellent applications in proteomic studies of halophytes.

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Section: The Bpp Protocol Produces More Protein Spotsmentioning

confidence: 94%

Section: The Bpp Methods Efficiently Removes Salt Ions From S Europaementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A protein extraction method compatible with proteomic analysis for the euhalophyte Salicornia europaea

et al. 2007

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Limited seed retention during winter inhibits vegetation establishment in spring, affecting lateral marsh expansion capacity

Regteren

Colosimo

Vries

et al. 2019

Ecology and Evolution

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Coastal systems worldwide deliver vital ecosystem services, such as biodiversity, carbon sequestration, and coastal protection. Effectivity of these ecosystem services increases when vegetation is present. Understanding the mechanisms behind vegetation establishment in bio‐geomorphic systems is necessary to understand their ability to recover after erosive events and potential adaptations to climate change. In this study, we examined how seed availability affects vegetation establishment in the salt marsh–intertidal flat transition zone: the area with capacity for lateral marsh expansion. This requires vegetation establishment; therefore, seed availability is essential. In a 6‐month field experiment, we simulated a before and after winter seed dispersal at two locations, the salt‐marsh vegetation edge and the intertidal flat, and studied seed retention, the seed bank, and the seed viability of three pioneer marsh species: Salicornia procumbens, Aster tripolium, and Spartina anglica. During winter storm conditions, all supplied seeds eroded away with the sediment surface layer. After winter, supplied seeds from all three species were retained, mostly at the surface while 9% was bioturbated downwards. In the natural seed bank, A. tripolium and S. anglica were practically absent while S. procumbens occurred more frequently. The viability of S. procumbens seeds was highest at the surface, between 80% and 90%. The viability quickly decreased with depth, although viable S. procumbens seeds occurred up to 15 cm depth. Only when seeds were supplied after winter, many S. procumbens and some S. anglica individuals did establish successfully in the transition zone. Viable seed availability formed a vegetation establishment threshold, even with a local seed source. Our results suggest that, although boundary conditions such as elevation, inundation, and weather conditions were appropriate for vegetation establishment in spring, the soil surface in winter can be so dynamic that it limits lateral marsh expansion. These insights can be used for designing effective nature‐based coastal protection.

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Biogeomorphic impact of oligochaetes (Annelida) on sediment properties and Salicornia spp. seedling establishment

et al. 2017

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Citation: van Regteren, M., R. ten Boer, E. H. Meesters, and A. V. de Groot. 2017. Biogeomorphic impact of oligochaetes (Annelida) on sediment properties and Salicornia spp. seedling establishment. Ecosphere 8(7):e01872. 10.1002/ecs2.1872Abstract. Oligochaetes (Annelida) are active bioturbators that can be present in high densities in the transition zone between intertidal flats and salt marshes, though their occurrence and functional role remain understudied. This study aimed to clarify the biogeomorphic role of oligochaete bioturbation in facilitating or hindering vegetation establishment. Two microcosm experiments were performed to assess the effect of oligochaete bioturbation on sediment properties, oxidation depth, algal biomass, seed distribution, and germination success of pioneer species Salicornia spp. Oligochaetes created burrow networks in the sediment matrix, which, together with upward conveyor belt feeding, lead to substrate mixing. Sediment reworking rates of oligochaetes were compared with those of polychaete macrofauna. Bioturbation and bio-irrigation of burrows can stimulate resource flows into the sediment. Oxidation depth increased almost tenfold in the presence of oligochaetes. Their bioturbation did not seem to affect sediment properties such as dry bulk density, porosity, and organic matter content. Sediment reworking, however, significantly reduced algal biomass at the surface with possible cascading effects on sediment stability and erodibility. Oligochaete conveyor belt feeding buried Salicornia spp. seeds until below the critical germination depth, thus negatively affecting Salicornia spp. germination and seedling establishment. Our study indicates that small, though numerous, oligochaete bioturbators may reduce lateral expansion potential of salt marshes by hindering the establishment of pioneer vegetation in the transition zone. Additionally, in dynamic fine-grained habitats, these oligochaetes have the feature to quickly oxygenate the sediment top layer.

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Salicornia L. (Salicornia pusilla J. Woods, S. ramosissima J. Woods, S. europaea L., S. obscura P.W. Ball & Tutin, S. nitens P.W. Ball & Tutin, S. fragilis P.W. Ball & Tutin and S. dolichostachya Moss)

Cited by 184 publications

References 143 publications

A protein extraction method compatible with proteomic analysis for the euhalophyte Salicornia europaea

A protein extraction method compatible with proteomic analysis for the euhalophyte Salicornia europaea

Limited seed retention during winter inhibits vegetation establishment in spring, affecting lateral marsh expansion capacity

Biogeomorphic impact of oligochaetes (Annelida) on sediment properties and Salicornia spp. seedling establishment

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