<i>Salmonella</i>
            biofilms program innate immunity for persistence in
            <i>Caenorhabditis elegans</i>

Desai, Stuti K.; Padmanabhan, Anup; Harshe, Sharvari; Zaidel-Bar, Ronen; Kenney, Linda J.

doi:10.1073/pnas.1822018116

Cited by 56 publications

(51 citation statements)

References 41 publications

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“…S. Typhimurium contains six OmpR binding sites upstream of the csgD transcriptional start site, one of which is reported to have an inhibitory effect on csgD expression (159). In Salmonella, OmpR also regulates the SsrA/B two-component system (112,113) and the RR SsrB non-canonically activates csgD in the absence of phosphorylation by derepressing H-NS (183)(184)(185).…”

Section: Ompr Is a Global Regulatormentioning

confidence: 99%

EnvZ/OmpR Two-Component Signaling: An Archetype System That Can Function Noncanonically

Kenney

Anand

2020

EcoSal Plus

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Two-component regulatory systems represent the major paradigm for signal transduction in prokaryotes. The simplest systems are composed of a sensor kinase and a response regulator. The sensor is often a membrane protein that senses a change in environmental conditions and is autophosphorylated by ATP on a histidine residue. The phosphoryl group is transferred onto an aspartate of the response regulator, which activates the regulator and alters its output, most often resulting in a change in gene expression. In this review, we present an historical view of the archetype EnvZ/OmpR two-component signaling system and then we provide a new view of signaling based on our recent experiments. EnvZ responds to cytoplasmic signals that arise from changes in the extracellular milieu (1), and OmpR acts canonically (requiring phosphorylation) to regulate the porin genes and non-canonically (without phosphorylation) to activate the acid stress response (2, 3). Herein we describe how insights gleaned from stimulus recognition and response in EnvZ are relevant to nearly all HK-RRs.

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Section: Ompr Is a Global Regulatormentioning

confidence: 99%

EnvZ/OmpR Two-Component Signaling: An Archetype System That Can Function Noncanonically

Kenney

Anand

2020

EcoSal Plus

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“…In vivo infection of mice [6][7][8] week-old female CBA/J mice, 129SvJ mice, or C57BL/6 mice were purchased from the Jackson Laboratories. The streptomycin-pretreated mouse model has been described previously [41].…”

Section: Detection Of Anti-dsdna Autoantibodiesmentioning

confidence: 99%

In vivo synthesis of bacterial amyloid curli contributes to joint inflammation during S. Typhimurium infection

Miller

Pasternak²,

Medeiros

et al. 2020

PLoS Pathog

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Reactive arthritis, an autoimmune disorder, occurs following gastrointestinal infection with invasive enteric pathogens, such as Salmonella enterica. Curli, an extracellular, bacterial amyloid with cross beta-sheet structure can trigger inflammatory responses by stimulating pattern recognition receptors. Here we show that S. Typhimurium produces curli amyloids in the cecum and colon of mice after natural oral infection, in both acute and chronic infection models. Production of curli was associated with an increase in anti-dsDNA autoantibodies and joint inflammation in infected mice. The negative impacts on the host appeared to be dependent on invasive systemic exposure of curli to immune cells. We hypothesize that in vivo synthesis of curli contributes to known complications of enteric infections and suggest that cross-seeding interactions can occur between pathogen-produced amyloids and amyloidogenic proteins of the host.

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“…The ΔyjiY sup treated WT cells showed signi cantly less invasion in Int407 cells. It was recently proposed that Salmonella persist in C. elegans gut by forming bio lm 38 . ΔyjiY supernatant treated WT cells showed a concomitant reduction in colonization in worms gut, suggesting an in vivo bio lm defect.…”

Section: Discussionmentioning

confidence: 99%

Global transcriptional regulation by cell-free sup of S. Typhimurium peptide transporter mutant leads to inhibition of intraspecies biofilm initiation

Chandra

Muralidhara²,

Kamanna

et al. 2020

Preprint

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Salmonella is a genus of widely spread Gram negative, facultative anaerobic bacteria, which is known to cause ¼th of the diarrhoeal morbidity and mortality globally. It causes typhoid fever and gastroenteritis by gaining access to the host gut through contaminated food and water. Salmonella utilizes its biofilm lifestyle to strongly resist antibiotics and persist in the host. Although the biofilm removal or dispersal have been studied widely, the inhibition of initiation Salmonella biofilm has not been studied much. This study was conducted to determine the anti-biofilm property of the cell-free supernatant obtained from a carbon-starvation inducible proline peptide transporter mutant (ΔyjiY) strain. Our study shows that Salmonella ΔyjiY culture supernatant inhibits biofilm initiation by regulating biofilm associated transcriptional network. This work demonstrates that highly abundant proteases like ecotin, HslV and GrpE cleave the protein aggregates, whereas global transcription regulators H-NS, FlgM regulate expression of SPIs and flagellar genes. Relatively low abundance of flavoredoxin, glutaredoxin, thiol peroxidase etc. leads to accumulation of ROS within the biofilm, and subsequent toxicity. This work further suggests that targeting these oxidative stress relieving proteins might be a good druggable choice to reduce Salmonella biofilm.

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Salmonella biofilms program innate immunity for persistence in Caenorhabditis elegans

Cited by 56 publications

References 41 publications

EnvZ/OmpR Two-Component Signaling: An Archetype System That Can Function Noncanonically

EnvZ/OmpR Two-Component Signaling: An Archetype System That Can Function Noncanonically

In vivo synthesis of bacterial amyloid curli contributes to joint inflammation during S. Typhimurium infection

Global transcriptional regulation by cell-free sup of S. Typhimurium peptide transporter mutant leads to inhibition of intraspecies biofilm initiation

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