Salmonella mediated the hemagglutinating virus of Japan-envelope transfer suppresses tumor growth

Abstract

Salmonella can target to tumor microenvironments after systemic treatment. The hemagglutinating virus of Japan-envelope (HVJ-E) induced apoptosis in tumor cells without toxicity in normal cells. Current HVJ-E therapeutic strategies, aimed at using HVJ-E for intratumor treatment, have shown great promise in animal models but have achieved only limited systemic treatment. The purpose of this study was to investigate the modulation of the anti-tumor efficiency of HVJ-E by coating the particles with poly (allylamine hydrochloride) (PAH), designated as P-HVJ-E. Treatment with P-HVJ-E resulted in decreased hemagglutinating activity and maintained tumor cell-selective apoptosis and anti-tumor immunity. The use of Salmonella as a coating for P-HVJ-E (PHS) enhanced the antitumor activity and maintained the tumor-targeting activity. Treatment with PHS resulted in delayed tumor growth in tumor-bearing mice. Furthermore, a Western blot assay of the tumors revealed that HVJ-E targeted to the tumor after systemic treatment with PHS. These results indicate that Salmonella coating viral particles may provide a new approach for tumor therapy.

Downregulations of AKT/mTOR Signaling Pathway for Salmonella-Mediated Suppression of Matrix Metalloproteinases-9 Expression in Mouse Tumor Models

“…Salmonella has an advantage over other antitumor agents because Salmonella could target to tumor sites [ 31 ]. Salmonella preferentially accumulated not only in primary tumors but also in metastatic nodules.…”

Salmonella Overcomes Drug Resistance in Tumor through P-glycoprotein Downregulation

“…Salmonella enterica serovar choleraesuis ( S. Choleraesuis; S.C.) (ATCC 15480) was obtained from Bioresources Collection and Research Center (Hsinchu, Taiwan) 10 . B16F10 (mouse melanoma) 11 and 4T1 (mouse breast cancer) 12 cells were maintained in culture dish with Dulbecco's Modified Eagle Medium containing 1% antibiotics (100 units/mL penicillin and 100 μg/mL streptomycin), 2mM l-glutamine and 10% fetal bovine serum.…”

Coating bacteria for anti-tumor therapy

“…The non-pathogenic parthenogenic anaerobic bacterium Salmonella can specifically target tumor sites to regulate immune response ( Pawelek et al, 1997 ; Lee et al, 2005 ; Zhao et al, 2005 ; Forbes, 2010 ; Chang and Lee, 2014 ). The modulation of the antitumor effect of Salmonella encapsulated with polyallylamine hydrochloride not only greatly enhanced antitumor activity but also maintained tumor targeting ( Lee et al, 2017 ).…”