Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H2O2

Li, Ying; Kang, Liying; Qi, Zihe; Gao, Xing; Quan, Huili; Lin, Hung-Ching

doi:10.3892/etm.2020.9496

Cited by 11 publications

(5 citation statements)

References 30 publications

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“…A variety of traditional Chinese medicines have obvious antioxidant effects, such as Hongjingtian ( Zhao et al, 2021 ) and Salvia miltiorrhiza ( Liang et al, 2021 ). The anti-oxidant tocopherol is a hydrolysis product of vitamin E that has the potential to scavenge free radicals.…”

Section: Introductionmentioning

confidence: 99%

Tocopherol attenuates the oxidative stress of BMSCs by inhibiting ferroptosis through the PI3k/AKT/mTOR pathway

Lan

Yao

et al. 2022

Front. Bioeng. Biotechnol.

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Oxidative stress can induce bone tissue damage and the occurrence of multiple diseases. As a type of traditional medicine, tocopherol has been reported to have a strong antioxidant effect and contributes to osteogenic differentiation. The purpose of this study was to investigate the protective effect of tocopherol on the oxidative stress of rat bone marrow-derived mesenchymal stem cells (BMSCs) and the underlying mechanisms. By establishing an oxidative stress model in vitro, the cell counting kit-8 (CCK-8), reactive oxygen species (ROS) analysis, Western blot (WB), real-time PCR (RT-PCR), alkaline phosphatase (ALP) staining, and Alizarin Red staining (ARS) evaluated the effects of tocopherol on the cell viability, intracellular ROS levels, and osteogenic differentiation in BMSCs. In addition, ferroptosis-related markers were examined via Western blot, RT-PCR, and Mito-FerroGreen. Eventually, the PI3K/AKT/mTOR signaling pathway was explored. We found that tocopherol significantly maintained the cell viability, reduced intracellular ROS levels, upregulated the levels of anti-oxidative genes, promoted the levels of osteogenic-related proteins, and the mRNA of BMSCs stimulated by H2O2. More importantly, tocopherol inhibited ferroptosis and upregulated the phosphorylation levels of PI3K, AKT, and mTOR of BMSCs upon H2O2 stimulation. In summary, tocopherol protected BMSCs from oxidative stress damage via the inhibition of ferroptosis through the PI3K/AKT/mTOR pathway.

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Section: Introductionmentioning

confidence: 99%

Tocopherol attenuates the oxidative stress of BMSCs by inhibiting ferroptosis through the PI3k/AKT/mTOR pathway

Lan

Yao

et al. 2022

Front. Bioeng. Biotechnol.

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“…The previous research above indicated that jasmonates (JAs) could regulate Tans and phenolic acids biosynthesis. Meanwhile, previous studies showed that JA could regulate SalB content in S. miltiorrhiza through cross-talk with brassinolide, Ca 2+ , and H 2 O 2 [10,11]. A previous study displayed that JA participated in PGA regulating the antioxidant capacity of S. miltiorrhiza [47].…”

Section: Discussionmentioning

confidence: 99%

“…In S. miltiorrhiza, the water-soluble ingredients have anticoagulation, cytoprotection, antiviral, antioxidation and other pharmacological effects [9]. The fat-soluble ingredients have antibacterial, anti-inflammatory, antitumor and other pharmacological effects [10,11]. Thus, the roots have been extensively used to treat inflammation, cardiovascular diseases, chronic renal failure, angina pectoris and dysmenorrhea in many regions in the world, mainly including Asia, Europe and the United States [2].…”

Section: Introductionmentioning

confidence: 99%

Poly-Glutamic Acid Promotes the Growth and the Accumulation of Main Medicinal Components in Salvia miltiorrhiza

Shan,

Zhang,

Luo

et al. 2024

Agronomy

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Salvia miltiorrhiza Bunge is a traditional medicinal plant in China and poly-glutamic acid (PGA) is a valuable biopolymer. However, it is unclear whether PGA promotes growth and the accumulation of main medicinal components in S. miltiorrhiza. To elucidate this scientific question, the influences of PGA on the growth, physiological characteristics, and accumulation of main medicinal components in S. miltiorrhiza were explored through a pot experiment. The results revealed that PGA significantly promoted basal diameter, plant height, shoot and root biomass, as well as root volume, compared with control. PGA also increased SPAD value, net photosynthetic rate, actual and maximum photochemical efficiency of photosynthetic system II, photochemical quenching, and electronic transfer rate. Meanwhile, PGA increased transpiration rate, stomatal conductance, water use efficiency, leaf relative water content, and the contents of soluble protein, soluble sugar, and proline. Furthermore, PGA increased the activities of antioxidant enzymes and the contents of antioxidants. The above findings imply that PGA facilitated S. miltiorrhiza growth by enhancing photosynthetic performance, water metabolism, and antioxidant capacity. Additionally, PGA significantly improved the yield of rosmarinic acid, salvianolic acid B, dihydrotanshinone, cryptotanshinone, tanshinone I, and tanshinone ⅡA in roots by up-regulating the transcript levels of genes responsible for their biosynthesis. Our findings indicated that PGA promoted S. miltiorrhiza growth and the accumulation of main medicinal components in roots.

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“…SAA and salvianolic acid B, the active components of S. miltiorrhiza , can inhibit the growth of cervical cancer cells [ 18 , 22 ]. Recent studies have shown that SAA could protect normal cells from damage, inhibit cardiomyocyte apoptosis in response to I/R [ 37 ], and ameliorate the human ovarian granulosa cell damage and human renal tubular epithelial cell injury induced by H 2 O 2 [ 38 , 39 ]. We studied the extract of S. miltiorrhiza to identify its effective components for treating cervical intraepithelial neoplasia.…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Salvia miltiorrhiza Extract and Its Active Components on Cervical Intraepithelial Neoplastic Cells

Leng

Kan

et al. 2022

Molecules

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The effective treatment of cervical intraepithelial neoplasia (CIN) can prevent cervical cancer. Salvia miltiorrhiza is a medicinal and health-promoting plant. To identify a potential treatment for CIN, the effect of S. miltiorrhiza extract and its active components on immortalized cervical epithelial cells was studied in vitro. The H8 cell was used as a CIN model. We found that S. miltiorrhiza extract effectively inhibited H8 cells through the CCK8 method. An HPLC–MS analysis revealed that S. miltiorrhiza extract contained salvianolic acid H, salvianolic acid A, salvianolic acid B, monomethyl lithospermate, 9′′′-methyl lithospermate B, and 9′′′-methyl lithospermate B/isomer. Salvianolic acid A had the best inhibitory effect on H8 cells with an IC50 value of 5.74 ± 0.63 μM. We also found that the combination of salvianolic acid A and oxysophoridine had a synergistic inhibitory effect on H8 cells at molar ratios of 4:1, 2:1, 1:1, 1:2, and 1:4, with salvianolic acid A/oxysophoridine = 1:2 having the best synergistic effect. Using Hoechst33342, flow cytometry, and Western blotting analysis, we found that the combination of salvianolic acid A and oxysophoridine can induce programmed apoptosis of H8 cells and block the cell cycle in the G2/M phase, which was correlated with decreased cyclinB1 and CDK1 protein levels. In conclusion, S. miltiorrhiza extract can inhibit the growth of H8 cells, and the combination of salvianolic acid A (its active component) and oxysophoridine has a synergistic inhibitory effect on H8 cells and may be a potential treatment for cervical intraepithelial neoplasia.

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Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H₂O₂

Cited by 11 publications

References 30 publications

Tocopherol attenuates the oxidative stress of BMSCs by inhibiting ferroptosis through the PI3k/AKT/mTOR pathway

Tocopherol attenuates the oxidative stress of BMSCs by inhibiting ferroptosis through the PI3k/AKT/mTOR pathway

Poly-Glutamic Acid Promotes the Growth and the Accumulation of Main Medicinal Components in Salvia miltiorrhiza

Inhibitory Effect of Salvia miltiorrhiza Extract and Its Active Components on Cervical Intraepithelial Neoplastic Cells

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