<i><scp>B</scp>artonella henselae</i> infection caused by cat flea bite

Oshima, Yuka; Fujii, Mari; Shiogama, Kazuya; Miyamoto, Kazuaki; Fujita, Hiromi; Sato, Shingo; Maruyama, Soichi; Mahara, Fumihiko; Tsutsumi, Yutaka

doi:10.1111/pin.12360

Cited by 7 publications

(3 citation statements)

References 9 publications

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“…Cervical lymphadenopathy in cat-scratch disease, infection of Bartonella henselae, is microscopically characterized by suppurative granuloma formation. Not only cat scratch but also bite by cat flea can provoke bartonellosis [41]. Splenic abscess is occasionally seen as systemic manifestation of cat-scratch disease [42].…”

Section: Application To Bartonellosismentioning

confidence: 99%

“…Splenectomy was performed from a male patient aged 40's. Monoclonal antibody H2A10 to B. henselae (not cross-reactive to non-henselae Bartonella) [41] demonstrated a few microbes in the cytoplasm of macrophages accumulated in the abscess cavity. The positive findings were more easily obtained by immunostaining with antisera against BCG and T. pallidum ( Figure 19) [19].…”

Section: Application To Bartonellosismentioning

confidence: 99%

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Low-Specificity and High-Sensitivity Immunostaining for Demonstrating Pathogens in Formalin-Fixed, Paraffin-Embedded Sections

Tsutsumi¹

2020

Immunohistochemistry - The Ageless Biotechnology

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The present review describes a part of the author's own experience in applying immunoperoxidase staining to routine histopathological diagnosis. The target disorder was focused on infection. In the practice of pathology diagnosis services, it is important for us diagnostic pathologists to judge whether the lesion is caused by an infection or not. When an infectious disease is highly likely, the visualization of pathogens within the inflammatory lesion is required to suggest a causative agent. Two main approaches the author would like to introduce include (1) the use of commercially available antisera showing wide cross-reactivity to a variety of bacteria and (2) the use of diluted patients' sera. These immunohistochemical studies employing "low-specificity" and "high-sensitivity" probes are useful for confirming the localization of pathogen within the infectious lesion.

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Section: Application To Bartonellosismentioning

confidence: 99%

Section: Application To Bartonellosismentioning

confidence: 99%

Low-Specificity and High-Sensitivity Immunostaining for Demonstrating Pathogens in Formalin-Fixed, Paraffin-Embedded Sections

Tsutsumi¹

2020

Immunohistochemistry - The Ageless Biotechnology

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“…The reservoir host of B. henselae is the house cat, Felis catus, and B. henselae is transmitted between cats by the faeces or flea 'dirt' of the cat flea, Ctenocephalides felis. The majority of human B. henselae infections are due to cat bites and scratches [64][65][66] , and is rarely transmitted to humans through flea faeces [67,68] . Though most cases of zoonotic B. henselae infection are in humans, B. henselae has also been known to infect dogs, horses, and porpoises as secondary hosts [69,70] .…”

Section: Bartonella Henselaementioning

confidence: 99%

Quantitative PCR Analysis of the Bartonella henselae carD Gene during Bacterial Stress

Simmonds¹

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<p>The Bartonella genus is comprised of arthropod-borne, intracellular bacterial pathogens that colonise the mammalian bloodstream. A large number of mammalian species are hosts for one or more Bartonella species, as either reservoir or incidental hosts. Bartonella species are only able to invade and replicate in host red blood cells in the reservoir host, and to be taken up by an associated haematophagous arthropod vector to complete transmission and the bacterial life cycle. Humans are the reservoir hosts for B. quintana and B. bacilliformis, and are incidental hosts for more than 16 additional zoonotic Bartonella species, including B. henselae, which is normally carried by cats. B. henselae infection, usually acquired through cat scratches or bites, can result in several clinical manifestations, with varying degrees of severity; the most common of these is cat scratch disease, where symptoms commonly range from enlarged lymph nodes to severe fever. Although usually a mild illness, B. henselae infection can occasionally lead to severe symptoms, affecting neurological and other major organ systems. During their life cycle the Bartonellae must adapt to various toxic host environments; such adaptation is mediated by several bacterial stress pathways, which modify bacterial transcription. However, many gaps remain in the understanding of B. henselae stress response pathways. The object of this study, the carD gene, was identified as a possible component of the Bartonella stress response. The carD gene has been shown to be critical for stress defence in other bacterial species, including Mycobacterium tuberculosis, Thermus thermophilus, and Myxococcus xanthus. Our study aimed to investigate whether carD played as significant a role during the B. henselae response to stresses as it does in other bacterial genera. We first attempted to perform growth comparisons between a B. henselae carD mutant strain and a wild type strain during exposure to stress conditions; however, our mutagenic carD plasmid interfered with bacterial growth of Escherichia coli cultures, which hindered transformation and generation of a B. henselae carD mutant. As an alternative, we investigated the expression of B. henselae carD under stress conditions, comparing carD expression during stress against a non-stressed B. henselae control, using quantitative PCR. We found no significant difference of expression of the carD gene between the control and any of our conditions, although a trend of increased carD expression was found in several stress conditions. We believe that these findings merit further study into the role of carD in the B. henselae stress response.</p>

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Molecular detection and identification of Bartonella species in cats from Hamedan and Kermanshah, Western Iran

Shamshiri¹,

Goudarztalejerdi²,

Zolhavarieh³

et al. 2022

Comparative Immunology, Microbiology and Infectious Diseases

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Bartonella henselae infection caused by cat flea bite

Cited by 7 publications

References 9 publications

Low-Specificity and High-Sensitivity Immunostaining for Demonstrating Pathogens in Formalin-Fixed, Paraffin-Embedded Sections

Low-Specificity and High-Sensitivity Immunostaining for Demonstrating Pathogens in Formalin-Fixed, Paraffin-Embedded Sections

Quantitative PCR Analysis of the Bartonella henselae carD Gene during Bacterial Stress

Molecular detection and identification of Bartonella species in cats from Hamedan and Kermanshah, Western Iran

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