<scp>B</scp>bmsn2 acts as a <scp>pH</scp>‐dependent negative regulator of secondary metabolite production in the entomopathogenic fungus <scp>B</scp>eauveria bassiana

Luo, Zhibing; Li, Yujie; Mousa, Jarrod J.; Bruner, Steven D.; Zhang, Yongjun; Pei, Yan; Keyhani, Nemat O.

doi:10.1111/1462-2920.12542

Cited by 47 publications

(56 citation statements)

References 54 publications

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“…S6D). Consistent with this finding, BbMsn2 (BBA_00971), a homolog of Msn2, has been identified as a negative regulator of oosporein production in B. bassiana (28). The regulation of oosporein biosynthesis thus represents a further example of fungal secondary metabolisms being cocontrolled by a pathway-specific transcription factor and a global regulator (29).…”

Section: Significancementioning

confidence: 59%

Fungal biosynthesis of the bibenzoquinone oosporein to evade insect immunity

Peng

Shang

Cen

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

206

176

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Quinones are widely distributed in nature and exhibit diverse biological or pharmacological activities; however, their biosynthetic machineries are largely unknown. The bibenzoquinone oosporein was first identified from the ascomycete insect pathogen Beauveria bassiana >50 y ago. The toxin can also be produced by different plant pathogenic and endophytic fungi with an array of biological activities. Here, we report the oosporein biosynthetic machinery in fungi, a polyketide synthase (PKS) pathway including seven genes for quinone biosynthesis. The PKS oosporein synthase 1 (OpS1) produces orsellinic acid that is hydroxylated to benzenetriol by the hydroxylase OpS4. The intermediate is oxidized either nonenzymatically to 5,5′-dideoxy-oosporein or enzymatically to benzenetetrol by the putative dioxygenase OpS7. The latter is further dimerized to oosporein by the catalase OpS5. The transcription factor OpS3 regulates intrapathway gene expression. Insect bioassays revealed that oosporein is required for fungal virulence and acts by evading host immunity to facilitate fungal multiplication in insects. These results contribute to the known mechanisms of quinone biosynthesis and the understanding of small molecules deployed by fungi that interact with their hosts.

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Section: Significancementioning

confidence: 59%

Fungal biosynthesis of the bibenzoquinone oosporein to evade insect immunity

Peng

Shang

Cen

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

206

176

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“…The PCR product was cloned into PCRBlunt and the integrity of the insert confirmed by sequencing. The complementation fragment in PCRBlunt was released by Spe I/ Xba I and cloned into the Xba I site of pK2‐ sur (Luo et al ., ) to form the vector pK2‐ sur‐BbpacC . The sequence containing complementation fragment and sur cassette was amplified using the primers P13/P14 and pK2‐ sur‐BbpacC as the template.…”

Section: Methodsmentioning

confidence: 99%

The PacC transcription factor regulates secondary metabolite production and stress response, but has only minor effects on virulence in the insect pathogenic fungus Beauveria bassiana

Luo¹,

Ren

Mousa

et al. 2017

Environmental Microbiology

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The PacC transcription factor is an important component of the fungal ambient pH-responsive regulatory system. Loss of pacC in the insect pathogenic fungus Beauveria bassiana resulted in an alkaline pH-dependent decrease in growth and pH-dependent increased susceptibility to osmotic (salt, sorbitol) stress and SDS. Extreme susceptibility to Congo Red was noted irrespective of pH, and ΔBbpacC conidia showed subtle increases in UV susceptibility. The ΔBbPacC mutant showed a reduced ability to acidify media during growth due to failure to produce oxalic acid. The ΔBbPacC mutant also did not produce the insecticidal compound dipicolinic acid, however, production of a yellow-colored compound was noted. The compound, named bassianolone B, was purified and its structure determined. Despite defects in growth, stress resistance, and oxalate/insecticidal compound production, only a small decrease in virulence was seen for the ΔBbpacC strain in topical insect bioassays using larvae from the greater waxmoth, Galleria mellonella or adults of the beetle, Tenebrio molitor. However, slightly more pronounced decreases were seen in virulence via intrahemcoel injection assays (G. mellonella) and in assays using T. molitor larvae. These data suggest important roles for BbpacC in mediating growth at alkaline pH, regulating secondary metabolite production, and in targeting specific insect stages.

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“…These results highlight the complexity of the regulated processes required for successful infection, and that simple derepression of cuticle degrading enzymes may be insufficient for the development of more virulent strains. The Msn2 transcription factor was shown to regulate, in response to external pH, the production of the secondary metabolite, oosporein, although the contributions of this compound to B. bassiana growth, development, or virulence remain unclear (Luo et al 2014a). Targeted deletion of Msn2 resulted in a strain impaired for growth at low pH, showing defects in virulence and sporulation.…”

Section: Insect and Plant Interactionsmentioning

confidence: 99%

Improving mycoinsecticides for insect biological control

Ortiz‐Urquiza

Luo

Keyhani

2014

Appl Microbiol Biotechnol

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149

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The desire for decreased reliance on chemical pesticides continues to fuel interest in alternative means for pest control including the use of naturally occurring microbial insect pathogens. Insects, as vectors of disease causing agents or as agricultural pests, are responsible for millions of deaths and significant economic losses worldwide, placing stresses on productivity (GDP) and human health and welfare. In addition, alterations in climate change are likely to affect insect ranges, expanding their access to previously constrained geographic areas, a potentially worrisome outcome. Metarhizium anisopliae and Beauveria bassiana, two cosmopolitan fungal pathogens of insects found in almost all ecosystems, are the most commonly applied mycoinsecticides for a variety of insect control purposes. The availability of the complete genomes for both organisms coupled to robust technologies for their transformation has led to several advances in engineering these fungi for greater efficacy and/or utility in pest control applications. Here, we will provide an overview of the fungal-insect and fungal-plant interactions that occur and highlight recent advances in the genetic engineering of these fungi. The latter work has resulted in the development of strains displaying (1) increased resistance to abiotic stress, (2) increased cuticular targeting and degradation, (3) increased virulence via expression of insecticidal protein/peptide toxins, (4) the ability to block transmission of disease causing agents, and (5) the ability to target specific insect hosts, decrease host fecundity, and/or alter insect behaviors.

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Bbmsn2 acts as a pH‐dependent negative regulator of secondary metabolite production in the entomopathogenic fungus Beauveria bassiana

Cited by 47 publications

References 54 publications

Fungal biosynthesis of the bibenzoquinone oosporein to evade insect immunity

Fungal biosynthesis of the bibenzoquinone oosporein to evade insect immunity

The PacC transcription factor regulates secondary metabolite production and stress response, but has only minor effects on virulence in the insect pathogenic fungus Beauveria bassiana

Improving mycoinsecticides for insect biological control

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